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Selection of a high-affinity DNA pool for a bZip protein with an out-of-phase alignment of the basic region relative to the leucine zipper.
Bioorg Med Chem. 2001 Sep; 9(9):2335-9.BM

Abstract

bZip transcription factors contain two regions that are required for DNA binding: a leucine zipper dimerization domain and a highly charged basic region that directly contacts DNA. The spacing between these subdomains is strictly conserved, and changes in this spacing result in a loss of function. Using an in vitro selection strategy, we have investigated the ability of a bZip protein with incorrect spacing between these two regions to bind specifically to DNA. Surprisingly, we find that although such a protein does not bind to its predicted site, it is possible to isolate a pool of DNAs that bind with very similar affinity to that of GCN4 for its optimum DNA site.

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Authors+Show Affiliations

Lee Y
Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, IN 47405-7102, USA.
Gurnon DG
No affiliation info available
Hollenbeck JJ
No affiliation info available
Oakley MG
No affiliation info available

MeSH

Amino Acid SequenceBasic-Leucine Zipper Transcription FactorsBinding SitesDNADNA-Binding ProteinsElectrophoretic Mobility Shift AssayFungal ProteinsG-Box Binding FactorsLeucine ZippersMolecular Sequence DataOligodeoxyribonucleotidesPeptide FragmentsProtein BindingProtein ConformationProtein KinasesSaccharomyces cerevisiae ProteinsStructure-Activity RelationshipTranscription FactorsYeasts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11553473

Citation

Lee, Y, et al. "Selection of a High-affinity DNA Pool for a bZip Protein With an Out-of-phase Alignment of the Basic Region Relative to the Leucine Zipper." Bioorganic & Medicinal Chemistry, vol. 9, no. 9, 2001, pp. 2335-9.
Lee Y, Gurnon DG, Hollenbeck JJ, et al. Selection of a high-affinity DNA pool for a bZip protein with an out-of-phase alignment of the basic region relative to the leucine zipper. Bioorg Med Chem. 2001;9(9):2335-9.
Lee, Y., Gurnon, D. G., Hollenbeck, J. J., & Oakley, M. G. (2001). Selection of a high-affinity DNA pool for a bZip protein with an out-of-phase alignment of the basic region relative to the leucine zipper. Bioorganic & Medicinal Chemistry, 9(9), 2335-9.
Lee Y, et al. Selection of a High-affinity DNA Pool for a bZip Protein With an Out-of-phase Alignment of the Basic Region Relative to the Leucine Zipper. Bioorg Med Chem. 2001;9(9):2335-9. PubMed PMID: 11553473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selection of a high-affinity DNA pool for a bZip protein with an out-of-phase alignment of the basic region relative to the leucine zipper. AU - Lee,Y, AU - Gurnon,D G, AU - Hollenbeck,J J, AU - Oakley,M G, PY - 2001/9/13/pubmed PY - 2002/4/9/medline PY - 2001/9/13/entrez SP - 2335 EP - 9 JF - Bioorganic & medicinal chemistry JO - Bioorg Med Chem VL - 9 IS - 9 N2 - bZip transcription factors contain two regions that are required for DNA binding: a leucine zipper dimerization domain and a highly charged basic region that directly contacts DNA. The spacing between these subdomains is strictly conserved, and changes in this spacing result in a loss of function. Using an in vitro selection strategy, we have investigated the ability of a bZip protein with incorrect spacing between these two regions to bind specifically to DNA. Surprisingly, we find that although such a protein does not bind to its predicted site, it is possible to isolate a pool of DNAs that bind with very similar affinity to that of GCN4 for its optimum DNA site. SN - 0968-0896 UR - https://www.unboundmedicine.com/medline/citation/11553473/Selection_of_a_high_affinity_DNA_pool_for_a_bZip_protein_with_an_out_of_phase_alignment_of_the_basic_region_relative_to_the_leucine_zipper_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0968089601001584 DB - PRIME DP - Unbound Medicine ER -