Tags

Type your tag names separated by a space and hit enter

Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp.
Infect Immun 2001; 69(10):6264-70II

Abstract

In this study, we evaluated the ability of DNA vaccines encoding the bacterioferritin (BFR) or P39 proteins of Brucella spp. to induce cellular and humoral immune responses and to protect BALB/c mice against a challenge with B. abortus 544. We constructed eukaryotic expression vectors called pCIBFR and pCIP39, encoding BFR or P39 antigens, respectively, and we verified that these proteins were produced after transfection of COS-7 cells. PCIBFR or pCIP39 was injected intramuscularly three times, at 3-week intervals. pCIP39 induced higher antibody responses than did the DNA vector encoding BFR. Both vectors elicited a T-cell-proliferative response and also induced a strong gamma interferon production upon restimulation with either the specific antigens or Brucella extract. In this report, we also demonstrate that animals immunized with these plasmids elicited a strong and long-lived memory immune response which persisted at least 3 months after the third vaccination. Furthermore, pCIBFR and pCIP39 induced a typical T-helper 1-dominated immune response in mice, as determined by cytokine or immunoglobulin G isotype analysis. The pCIP39 delivered by intramuscular injection (but not the pCIBFR or control vectors) induced a moderate protection in BALB/c mice challenged with B. abortus 544 compared to that observed in positive control mice vaccinated with S19.

Authors+Show Affiliations

Unité de Recherche en Biologie Moléculaire, Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11553569

Citation

Al-Mariri, A, et al. "Induction of Immune Response in BALB/c Mice With a DNA Vaccine Encoding Bacterioferritin or P39 of Brucella Spp." Infection and Immunity, vol. 69, no. 10, 2001, pp. 6264-70.
Al-Mariri A, Tibor A, Mertens P, et al. Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp. Infect Immun. 2001;69(10):6264-70.
Al-Mariri, A., Tibor, A., Mertens, P., De Bolle, X., Michel, P., Godfroid, J., ... Letesson, J. J. (2001). Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp. Infection and Immunity, 69(10), pp. 6264-70.
Al-Mariri A, et al. Induction of Immune Response in BALB/c Mice With a DNA Vaccine Encoding Bacterioferritin or P39 of Brucella Spp. Infect Immun. 2001;69(10):6264-70. PubMed PMID: 11553569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp. AU - Al-Mariri,A, AU - Tibor,A, AU - Mertens,P, AU - De Bolle,X, AU - Michel,P, AU - Godfroid,J, AU - Walravens,K, AU - Letesson,J J, PY - 2001/9/13/pubmed PY - 2001/10/26/medline PY - 2001/9/13/entrez SP - 6264 EP - 70 JF - Infection and immunity JO - Infect. Immun. VL - 69 IS - 10 N2 - In this study, we evaluated the ability of DNA vaccines encoding the bacterioferritin (BFR) or P39 proteins of Brucella spp. to induce cellular and humoral immune responses and to protect BALB/c mice against a challenge with B. abortus 544. We constructed eukaryotic expression vectors called pCIBFR and pCIP39, encoding BFR or P39 antigens, respectively, and we verified that these proteins were produced after transfection of COS-7 cells. PCIBFR or pCIP39 was injected intramuscularly three times, at 3-week intervals. pCIP39 induced higher antibody responses than did the DNA vector encoding BFR. Both vectors elicited a T-cell-proliferative response and also induced a strong gamma interferon production upon restimulation with either the specific antigens or Brucella extract. In this report, we also demonstrate that animals immunized with these plasmids elicited a strong and long-lived memory immune response which persisted at least 3 months after the third vaccination. Furthermore, pCIBFR and pCIP39 induced a typical T-helper 1-dominated immune response in mice, as determined by cytokine or immunoglobulin G isotype analysis. The pCIP39 delivered by intramuscular injection (but not the pCIBFR or control vectors) induced a moderate protection in BALB/c mice challenged with B. abortus 544 compared to that observed in positive control mice vaccinated with S19. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/11553569/Induction_of_immune_response_in_BALB/c_mice_with_a_DNA_vaccine_encoding_bacterioferritin_or_P39_of_Brucella_spp_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=11553569 DB - PRIME DP - Unbound Medicine ER -