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Two transporters, TogT and TogMNAB, are responsible for oligogalacturonide uptake in Erwinia chrysanthemi 3937.
Mol Microbiol. 2001 Sep; 41(5):1125-32.MM

Abstract

Erwinia chrysanthemi causes soft rot of plants by secreting pectinases which cleave pectin, a polysaccharide cementing the plant cell wall constituents. We demonstrated that two transporters mediate the uptake of the extracellularly formed oligomers in E. chrysanthemi. TogMNAB, a multicomponent transporter member of the ATP-binding cassette (ABC) superfamily, is only partially responsible for the uptake of pectic oligomers. Its action is completed by that of the second transporter, TogT, a member of the glycoside-pentoside-hexuronide (GPH) family (TC no. 2.2) which includes transporters involved in the uptake of complex sugars, mostly oligosaccharides and glycosides. Each transport system, TogMNAB and TogT, is able to independently mediate the transport of oligogalacturonides and the simultaneous inactivation of both is necessary to give a total absence of growth with pectin as the carbon source. The togT gene constitutes an independent transcriptional unit. Its expression is induced in the presence of pectic derivatives and it is subject to catabolite repression. In vitro, the repressor KdgR and the activator CRP both interact directly with the togT regulatory region. The decreased pathogenicity of single and double togT, togM mutants indicated that a deficiency in uptake of pectic oligomers leads to reduced bacterial multiplication which, in turn, limits plant maceration.

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Authors+Show Affiliations

Hugouvieux-Cotte-Pattat N
Unité Microbiologie et Génétique--composante INSA, UMR UCB-INSA-CNRS 5122, Bat Louis Pasteur, INSA, 11 Avenue Jean Capelle, F-69621 Villeurbanne Cedex, France. cotte@insa-lyon.fr
Reverchon S
No affiliation info available

MeSH

ATP-Binding Cassette TransportersBacterial ProteinsBase SequenceBinding SitesBiological Transport, ActiveCarbohydrate SequenceCarrier ProteinsCyclic AMP Receptor ProteinDickeya chrysanthemiGene Expression Regulation, BacterialMolecular Sequence DataMonosaccharide Transport ProteinsMutationOligosaccharidesPectinsRepressor ProteinsTranscription FactorsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11555292

Citation

Hugouvieux-Cotte-Pattat, N, and S Reverchon. "Two Transporters, TogT and TogMNAB, Are Responsible for Oligogalacturonide Uptake in Erwinia Chrysanthemi 3937." Molecular Microbiology, vol. 41, no. 5, 2001, pp. 1125-32.
Hugouvieux-Cotte-Pattat N, Reverchon S. Two transporters, TogT and TogMNAB, are responsible for oligogalacturonide uptake in Erwinia chrysanthemi 3937. Mol Microbiol. 2001;41(5):1125-32.
Hugouvieux-Cotte-Pattat, N., & Reverchon, S. (2001). Two transporters, TogT and TogMNAB, are responsible for oligogalacturonide uptake in Erwinia chrysanthemi 3937. Molecular Microbiology, 41(5), 1125-32.
Hugouvieux-Cotte-Pattat N, Reverchon S. Two Transporters, TogT and TogMNAB, Are Responsible for Oligogalacturonide Uptake in Erwinia Chrysanthemi 3937. Mol Microbiol. 2001;41(5):1125-32. PubMed PMID: 11555292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two transporters, TogT and TogMNAB, are responsible for oligogalacturonide uptake in Erwinia chrysanthemi 3937. AU - Hugouvieux-Cotte-Pattat,N, AU - Reverchon,S, PY - 2001/9/14/pubmed PY - 2002/2/12/medline PY - 2001/9/14/entrez SP - 1125 EP - 32 JF - Molecular microbiology JO - Mol Microbiol VL - 41 IS - 5 N2 - Erwinia chrysanthemi causes soft rot of plants by secreting pectinases which cleave pectin, a polysaccharide cementing the plant cell wall constituents. We demonstrated that two transporters mediate the uptake of the extracellularly formed oligomers in E. chrysanthemi. TogMNAB, a multicomponent transporter member of the ATP-binding cassette (ABC) superfamily, is only partially responsible for the uptake of pectic oligomers. Its action is completed by that of the second transporter, TogT, a member of the glycoside-pentoside-hexuronide (GPH) family (TC no. 2.2) which includes transporters involved in the uptake of complex sugars, mostly oligosaccharides and glycosides. Each transport system, TogMNAB and TogT, is able to independently mediate the transport of oligogalacturonides and the simultaneous inactivation of both is necessary to give a total absence of growth with pectin as the carbon source. The togT gene constitutes an independent transcriptional unit. Its expression is induced in the presence of pectic derivatives and it is subject to catabolite repression. In vitro, the repressor KdgR and the activator CRP both interact directly with the togT regulatory region. The decreased pathogenicity of single and double togT, togM mutants indicated that a deficiency in uptake of pectic oligomers leads to reduced bacterial multiplication which, in turn, limits plant maceration. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/11555292/Two_transporters_TogT_and_TogMNAB_are_responsible_for_oligogalacturonide_uptake_in_Erwinia_chrysanthemi_3937_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2001&volume=41&issue=5&spage=1125 DB - PRIME DP - Unbound Medicine ER -