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Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels.
Southeast Asian J Trop Med Public Health. 2001 Jun; 32(2):308-13.SA

Abstract

A 31-kDa glycoprotein antigen was purified by electrophoresing the crude extract of Parastrongylus cantonensis adult worms in a 12% SDS-polyacrylamide gel, identifying the 31-kDa component with prestained molecular weight standards, cutting the desired gel strip, and then isolating it by electroelution. Antigen fraction of 31 kDa was re-electrophoresed, transferred to a nitrocellulose membrane and found to be reactive with only the sera from patients with parastrongyliasis. No reactive band was observed with the sera from other related parasitic infections, eg, gnathostomiasis, toxocariasis, filariasis, paragonimiasis, cysticercosis and malaria, and the normal healthy control sera. This antigen fraction isolated showed 100% sensitivity and 100% specificity in the enzyme-linked immunosorbent assay (ELISA) for the detection of 31-kDa specific antibody in the sera from patients with parastrongyliasis. The P. cantonensis antigen of 31 kDa has been obtained by this means with a high degree of purity and applied successfully in conventional ELISA for the specific immunodiagnosis of human parastrongyliasis.

Authors+Show Affiliations

Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11556581

Citation

Eamsobhana, P, et al. "Purification of a Specific Immunodiagnostic Parastrongylus Cantonensis Antigen By Electroelution From SDS-polyacrylamide Gels." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 32, no. 2, 2001, pp. 308-13.
Eamsobhana P, Yoolek A, Suvouttho S, et al. Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels. Southeast Asian J Trop Med Public Health. 2001;32(2):308-13.
Eamsobhana, P., Yoolek, A., Suvouttho, S., & Suvouttho, S. (2001). Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels. The Southeast Asian Journal of Tropical Medicine and Public Health, 32(2), 308-13.
Eamsobhana P, et al. Purification of a Specific Immunodiagnostic Parastrongylus Cantonensis Antigen By Electroelution From SDS-polyacrylamide Gels. Southeast Asian J Trop Med Public Health. 2001;32(2):308-13. PubMed PMID: 11556581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels. AU - Eamsobhana,P, AU - Yoolek,A, AU - Suvouttho,S, AU - Suvouttho,S, PY - 2001/9/15/pubmed PY - 2002/1/10/medline PY - 2001/9/15/entrez SP - 308 EP - 13 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 32 IS - 2 N2 - A 31-kDa glycoprotein antigen was purified by electrophoresing the crude extract of Parastrongylus cantonensis adult worms in a 12% SDS-polyacrylamide gel, identifying the 31-kDa component with prestained molecular weight standards, cutting the desired gel strip, and then isolating it by electroelution. Antigen fraction of 31 kDa was re-electrophoresed, transferred to a nitrocellulose membrane and found to be reactive with only the sera from patients with parastrongyliasis. No reactive band was observed with the sera from other related parasitic infections, eg, gnathostomiasis, toxocariasis, filariasis, paragonimiasis, cysticercosis and malaria, and the normal healthy control sera. This antigen fraction isolated showed 100% sensitivity and 100% specificity in the enzyme-linked immunosorbent assay (ELISA) for the detection of 31-kDa specific antibody in the sera from patients with parastrongyliasis. The P. cantonensis antigen of 31 kDa has been obtained by this means with a high degree of purity and applied successfully in conventional ELISA for the specific immunodiagnosis of human parastrongyliasis. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/11556581/Purification_of_a_specific_immunodiagnostic_Parastrongylus_cantonensis_antigen_by_electroelution_from_SDS_polyacrylamide_gels_ DB - PRIME DP - Unbound Medicine ER -