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Identification of new sites of binding and activation of the murine alpha1(I) collagen promoter by CCAAT/enhancer binding protein beta.
DNA Cell Biol. 2001 Aug; 20(8):455-63.DC

Abstract

The CCAAT/enhancer binding protein beta (C/EBPbeta) was previously shown to bind to the alpha(1)(I) collagen promoter at -365 to -335 (site 1) and to activate it. Acetaldehyde also activates the promoter, and this effect is mediated by an increase in stellate-cell C/EBPbeta protein and C/EBPbeta binding. The present study identified two additional distal sites (sites 2 and 3) of binding of C/EBPbeta, in the nuclear extracts of stellate cells, at -399 to -370 and -623 to -592 in the alpha(1)(I) collagen promoter. The C/EBPbeta protein activates the promoter at all three sites. Acetaldehyde increases C/EBPbeta binding to all three sites. Activation by acetaldehyde is abrogated in the transfected promoter mutated at either site 1 or site 3 but is not affected by mutation at site 2. Binding of the 20-kDa C/EBPbeta isoform (p20C/EBPbeta), which is eliminated by mutation at the distal site 3 of C/EBP binding, is necessary for the activation by acetaldehyde of the alpha(1)(I) collagen promoter.

Authors+Show Affiliations

Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2195, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11560777

Citation

Attard, F A., et al. "Identification of New Sites of Binding and Activation of the Murine alpha1(I) Collagen Promoter By CCAAT/enhancer Binding Protein Beta." DNA and Cell Biology, vol. 20, no. 8, 2001, pp. 455-63.
Attard FA, Wang L, Potter JJ, et al. Identification of new sites of binding and activation of the murine alpha1(I) collagen promoter by CCAAT/enhancer binding protein beta. DNA Cell Biol. 2001;20(8):455-63.
Attard, F. A., Wang, L., Potter, J. J., Rennie-Tankersley, L., & Mezey, E. (2001). Identification of new sites of binding and activation of the murine alpha1(I) collagen promoter by CCAAT/enhancer binding protein beta. DNA and Cell Biology, 20(8), 455-63.
Attard FA, et al. Identification of New Sites of Binding and Activation of the Murine alpha1(I) Collagen Promoter By CCAAT/enhancer Binding Protein Beta. DNA Cell Biol. 2001;20(8):455-63. PubMed PMID: 11560777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of new sites of binding and activation of the murine alpha1(I) collagen promoter by CCAAT/enhancer binding protein beta. AU - Attard,F A, AU - Wang,L, AU - Potter,J J, AU - Rennie-Tankersley,L, AU - Mezey,E, PY - 2001/9/19/pubmed PY - 2001/10/12/medline PY - 2001/9/19/entrez SP - 455 EP - 63 JF - DNA and cell biology JO - DNA Cell Biol VL - 20 IS - 8 N2 - The CCAAT/enhancer binding protein beta (C/EBPbeta) was previously shown to bind to the alpha(1)(I) collagen promoter at -365 to -335 (site 1) and to activate it. Acetaldehyde also activates the promoter, and this effect is mediated by an increase in stellate-cell C/EBPbeta protein and C/EBPbeta binding. The present study identified two additional distal sites (sites 2 and 3) of binding of C/EBPbeta, in the nuclear extracts of stellate cells, at -399 to -370 and -623 to -592 in the alpha(1)(I) collagen promoter. The C/EBPbeta protein activates the promoter at all three sites. Acetaldehyde increases C/EBPbeta binding to all three sites. Activation by acetaldehyde is abrogated in the transfected promoter mutated at either site 1 or site 3 but is not affected by mutation at site 2. Binding of the 20-kDa C/EBPbeta isoform (p20C/EBPbeta), which is eliminated by mutation at the distal site 3 of C/EBP binding, is necessary for the activation by acetaldehyde of the alpha(1)(I) collagen promoter. SN - 1044-5498 UR - https://www.unboundmedicine.com/medline/citation/11560777/Identification_of_new_sites_of_binding_and_activation_of_the_murine_alpha1_I__collagen_promoter_by_CCAAT/enhancer_binding_protein_beta_ DB - PRIME DP - Unbound Medicine ER -