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Oxalate accumulation from citrate by Aspergillus niger. II. Involvement of the tricarboxylic acid cyclase.
Arch Microbiol. 1975 Jun 22; 104(2):159-62.AM

Abstract

Carbon-14 was incorporated into oxalate and CO2 from either citrate-1,5-14C, succinate-1,4-14C, or fumarate-1,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium forming oxalate and CO2 from added citrate the following enzymes of the tricarboxylic acid (TCA) cycle were identified: citrate synthase CE 4.1.3.7), aconitate hydratase (EC4.2.1.3), NAD and NADP-dependent isocitrate dehydrogenase (EC 1.1.1.41, 1.1.1.42), (alpha-oxoglutarate dehydrogenase (EC 1.2.4.2), succinate dehydrogenase (EC 1.3.99.1), fumarate hydratase (EC 4.2.1.2), and malate dehydrogenase (EC 1.1.1.37). The in vitro activity of aconitate hydratase and of NADP-dependent isocitrate dehydrogenase was shown to be almost identical to the rate of in vivo degradation of citrate or to exceed this rate. The degradation of citrate to oxalate was inhibited completely by 9 mM fluoroacetate. It is concluded that the TCA cycle is involved in the formation of oxalate from citrate.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1156100

Citation

Müller, H M., and S Frosch. "Oxalate Accumulation From Citrate By Aspergillus Niger. II. Involvement of the Tricarboxylic Acid Cyclase." Archives of Microbiology, vol. 104, no. 2, 1975, pp. 159-62.
Müller HM, Frosch S. Oxalate accumulation from citrate by Aspergillus niger. II. Involvement of the tricarboxylic acid cyclase. Arch Microbiol. 1975;104(2):159-62.
Müller, H. M., & Frosch, S. (1975). Oxalate accumulation from citrate by Aspergillus niger. II. Involvement of the tricarboxylic acid cyclase. Archives of Microbiology, 104(2), 159-62.
Müller HM, Frosch S. Oxalate Accumulation From Citrate By Aspergillus Niger. II. Involvement of the Tricarboxylic Acid Cyclase. Arch Microbiol. 1975 Jun 22;104(2):159-62. PubMed PMID: 1156100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxalate accumulation from citrate by Aspergillus niger. II. Involvement of the tricarboxylic acid cyclase. AU - Müller,H M, AU - Frosch,S, PY - 1975/6/22/pubmed PY - 1975/6/22/medline PY - 1975/6/22/entrez SP - 159 EP - 62 JF - Archives of microbiology JO - Arch Microbiol VL - 104 IS - 2 N2 - Carbon-14 was incorporated into oxalate and CO2 from either citrate-1,5-14C, succinate-1,4-14C, or fumarate-1,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium forming oxalate and CO2 from added citrate the following enzymes of the tricarboxylic acid (TCA) cycle were identified: citrate synthase CE 4.1.3.7), aconitate hydratase (EC4.2.1.3), NAD and NADP-dependent isocitrate dehydrogenase (EC 1.1.1.41, 1.1.1.42), (alpha-oxoglutarate dehydrogenase (EC 1.2.4.2), succinate dehydrogenase (EC 1.3.99.1), fumarate hydratase (EC 4.2.1.2), and malate dehydrogenase (EC 1.1.1.37). The in vitro activity of aconitate hydratase and of NADP-dependent isocitrate dehydrogenase was shown to be almost identical to the rate of in vivo degradation of citrate or to exceed this rate. The degradation of citrate to oxalate was inhibited completely by 9 mM fluoroacetate. It is concluded that the TCA cycle is involved in the formation of oxalate from citrate. SN - 0302-8933 UR - https://www.unboundmedicine.com/medline/citation/1156100/Oxalate_accumulation_from_citrate_by_Aspergillus_niger__II__Involvement_of_the_tricarboxylic_acid_cyclase_ DB - PRIME DP - Unbound Medicine ER -