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Aberrant lipid organization in stratum corneum of patients with atopic dermatitis and lamellar ichthyosis.
J Invest Dermatol. 2001 Sep; 117(3):710-7.JI

Abstract

There are several skin diseases in which the lipid composition in the intercellular matrix of the stratum corneum is different from that of healthy human skin. It has been shown that patients suffering from atopic dermatitis have a reduced ceramide content in the stratum corneum, whereas in the stratum corneum of lamellar ichthyosis patients, the amount of free fatty acids is decreased and the ceramide profile is altered. Both patient groups also show elevated levels of transepidermal water loss indicative of an impaired barrier function. As ceramides and free fatty acids are essential for a proper barrier function, we hypothesized that changes in the composition of these lipids would be reflected in the lipid organization in stratum corneum of atopic dermatitis and lamellar ichthyosis patients. We investigated the lateral lipid packing using electron diffraction and the lamellar organization using freeze fracture electron microscopy. In atopic dermatitis stratum corneum, we found that, in comparison with healthy stratum corneum, the presence of the hexagonal lattice (gel phase) is increased with respect to the orthorhombic packing (crystalline phase). In lamellar ichthyosis stratum corneum, the hexagonal packing was predominantly present, whereas the orthorhombic packing was observed only occasionally. This is in good agreement with studies on stratum corneum lipid models that show that the presence of long-chain free fatty acids is involved in the formation of the orthorhombic packing. The results of this study also suggest that the ceramide composition is important for the lateral lipid packing. Finally, using freeze fracture electron microscopy, changes in the lamellar organization in stratum corneum of both patient groups could be observed.

Authors+Show Affiliations

Center for Electron Microscopy, Leiden University Medical Center, Leiden, the Netherlands. G.S.K.Pilgram@bio.uu.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11564181

Citation

Pilgram, G S., et al. "Aberrant Lipid Organization in Stratum Corneum of Patients With Atopic Dermatitis and Lamellar Ichthyosis." The Journal of Investigative Dermatology, vol. 117, no. 3, 2001, pp. 710-7.
Pilgram GS, Vissers DC, van der Meulen H, et al. Aberrant lipid organization in stratum corneum of patients with atopic dermatitis and lamellar ichthyosis. J Invest Dermatol. 2001;117(3):710-7.
Pilgram, G. S., Vissers, D. C., van der Meulen, H., Pavel, S., Lavrijsen, S. P., Bouwstra, J. A., & Koerten, H. K. (2001). Aberrant lipid organization in stratum corneum of patients with atopic dermatitis and lamellar ichthyosis. The Journal of Investigative Dermatology, 117(3), 710-7.
Pilgram GS, et al. Aberrant Lipid Organization in Stratum Corneum of Patients With Atopic Dermatitis and Lamellar Ichthyosis. J Invest Dermatol. 2001;117(3):710-7. PubMed PMID: 11564181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aberrant lipid organization in stratum corneum of patients with atopic dermatitis and lamellar ichthyosis. AU - Pilgram,G S, AU - Vissers,D C, AU - van der Meulen,H, AU - Pavel,S, AU - Lavrijsen,S P, AU - Bouwstra,J A, AU - Koerten,H K, PY - 2001/9/21/pubmed PY - 2001/10/12/medline PY - 2001/9/21/entrez SP - 710 EP - 7 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 117 IS - 3 N2 - There are several skin diseases in which the lipid composition in the intercellular matrix of the stratum corneum is different from that of healthy human skin. It has been shown that patients suffering from atopic dermatitis have a reduced ceramide content in the stratum corneum, whereas in the stratum corneum of lamellar ichthyosis patients, the amount of free fatty acids is decreased and the ceramide profile is altered. Both patient groups also show elevated levels of transepidermal water loss indicative of an impaired barrier function. As ceramides and free fatty acids are essential for a proper barrier function, we hypothesized that changes in the composition of these lipids would be reflected in the lipid organization in stratum corneum of atopic dermatitis and lamellar ichthyosis patients. We investigated the lateral lipid packing using electron diffraction and the lamellar organization using freeze fracture electron microscopy. In atopic dermatitis stratum corneum, we found that, in comparison with healthy stratum corneum, the presence of the hexagonal lattice (gel phase) is increased with respect to the orthorhombic packing (crystalline phase). In lamellar ichthyosis stratum corneum, the hexagonal packing was predominantly present, whereas the orthorhombic packing was observed only occasionally. This is in good agreement with studies on stratum corneum lipid models that show that the presence of long-chain free fatty acids is involved in the formation of the orthorhombic packing. The results of this study also suggest that the ceramide composition is important for the lateral lipid packing. Finally, using freeze fracture electron microscopy, changes in the lamellar organization in stratum corneum of both patient groups could be observed. SN - 0022-202X UR - https://www.unboundmedicine.com/medline/citation/11564181/Aberrant_lipid_organization_in_stratum_corneum_of_patients_with_atopic_dermatitis_and_lamellar_ichthyosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)41370-3 DB - PRIME DP - Unbound Medicine ER -