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Aging alters neuronal nitric oxide release from rat mesenteric arteries: role of presynaptic beta-adrenoceptors.
Clin Sci (Lond). 2001 Oct; 101(4):321-8.CS

Abstract

This study examines the influence of aging on the neuronal nitric oxide (NO) and noradrenaline (NA) release elicited by electrical field stimulation (EFS; 200 mA, 0.3 ms, 1-16 Hz, 30 s) in endothelium-denuded mesenteric arteries from young and old rats, as well as the influence of the presynaptic beta-adrenoceptors in that release. EFS induced frequency-dependent contractions. N(G)-nitro-l-arginine methyl ester (l-NAME) only enhanced EFS-elicited contractions in arteries from young rats. Capsaicin did not alter the EFS-induced contractions in either age group. Clenbuterol did not modify the contraction elicited by EFS in arteries from young or old rats either. A subsequent addition of l-NAME also induced an increase in the EFS-induced response in arteries from both age groups. In old rats, the presence of propranolol did not alter the response induced by EFS, and the subsequent addition of clenbuterol or clenbuterol plus l-NAME did not affect this response. In precontracted segments, sodium nitroprusside or clenbuterol induced similar relaxation in both age groups. None of the drugs used altered the response to exogenous NA or basal tone. In arteries preincubated with [(3)H]NA, EFS induced (3)H release, which remained unmodified in the presence of clenbuterol or propranolol in young rats, whereas clenbuterol increased the (3)H overflow in old rats, and this effect was abolished by propranolol. These drugs did not alter the basal (3)H efflux and indicate that in rat mesenteric arteries EFS induces NA release in both age groups, and only NO release in young animals. Activation of presynaptic beta-adrenoceptors increased NA and probably NO release in aged rats.

Authors+Show Affiliations

Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, C/Arzobispo Morcillo 4, 28029-Madrid, Spain.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11566067

Citation

Ferrer, M, and G Balfagón. "Aging Alters Neuronal Nitric Oxide Release From Rat Mesenteric Arteries: Role of Presynaptic Beta-adrenoceptors." Clinical Science (London, England : 1979), vol. 101, no. 4, 2001, pp. 321-8.
Ferrer M, Balfagón G. Aging alters neuronal nitric oxide release from rat mesenteric arteries: role of presynaptic beta-adrenoceptors. Clin Sci (Lond). 2001;101(4):321-8.
Ferrer, M., & Balfagón, G. (2001). Aging alters neuronal nitric oxide release from rat mesenteric arteries: role of presynaptic beta-adrenoceptors. Clinical Science (London, England : 1979), 101(4), 321-8.
Ferrer M, Balfagón G. Aging Alters Neuronal Nitric Oxide Release From Rat Mesenteric Arteries: Role of Presynaptic Beta-adrenoceptors. Clin Sci (Lond). 2001;101(4):321-8. PubMed PMID: 11566067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aging alters neuronal nitric oxide release from rat mesenteric arteries: role of presynaptic beta-adrenoceptors. AU - Ferrer,M, AU - Balfagón,G, PY - 2001/9/22/pubmed PY - 2002/4/19/medline PY - 2001/9/22/entrez SP - 321 EP - 8 JF - Clinical science (London, England : 1979) JO - Clin Sci (Lond) VL - 101 IS - 4 N2 - This study examines the influence of aging on the neuronal nitric oxide (NO) and noradrenaline (NA) release elicited by electrical field stimulation (EFS; 200 mA, 0.3 ms, 1-16 Hz, 30 s) in endothelium-denuded mesenteric arteries from young and old rats, as well as the influence of the presynaptic beta-adrenoceptors in that release. EFS induced frequency-dependent contractions. N(G)-nitro-l-arginine methyl ester (l-NAME) only enhanced EFS-elicited contractions in arteries from young rats. Capsaicin did not alter the EFS-induced contractions in either age group. Clenbuterol did not modify the contraction elicited by EFS in arteries from young or old rats either. A subsequent addition of l-NAME also induced an increase in the EFS-induced response in arteries from both age groups. In old rats, the presence of propranolol did not alter the response induced by EFS, and the subsequent addition of clenbuterol or clenbuterol plus l-NAME did not affect this response. In precontracted segments, sodium nitroprusside or clenbuterol induced similar relaxation in both age groups. None of the drugs used altered the response to exogenous NA or basal tone. In arteries preincubated with [(3)H]NA, EFS induced (3)H release, which remained unmodified in the presence of clenbuterol or propranolol in young rats, whereas clenbuterol increased the (3)H overflow in old rats, and this effect was abolished by propranolol. These drugs did not alter the basal (3)H efflux and indicate that in rat mesenteric arteries EFS induces NA release in both age groups, and only NO release in young animals. Activation of presynaptic beta-adrenoceptors increased NA and probably NO release in aged rats. SN - 0143-5221 UR - https://www.unboundmedicine.com/medline/citation/11566067/Aging_alters_neuronal_nitric_oxide_release_from_rat_mesenteric_arteries:_role_of_presynaptic_beta_adrenoceptors_ L2 - https://medlineplus.gov/olderadulthealth.html DB - PRIME DP - Unbound Medicine ER -