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RRR-alpha-tocopheryl succinate induces MDA-MB-435 and MCF-7 human breast cancer cells to undergo differentiation.
Cell Growth Differ 2001; 12(9):471-80CG

Abstract

RRR-alpha-Tocopheryl succinate (vitamin E succinate, VES) is a potent antitumor agent, inducing DNA synthesis arrest, differentiation, and apoptosis. Because little is known about VES-induced differentiation, studies reported here characterize VES effects on the differentiation status of human breast cancer cell lines and investigate possible molecular mechanisms involved. VES-induced differentiation of human MCF-7 and MDA-MB-435 breast cancer cells was characterized by morphological changes, induction of lipid droplets, induction of beta-casein mRNA expression, and down-regulation of Her2/neu protein. In contrast, VES treatment of normal human mammary epithelial cells, MCF-10A cells, and T-47D cells did not induce differentiation. Studies addressing mechanisms showed that neither antibody neutralization of the transforming growth factor-beta signaling pathway nor expression of a dominant-negative mutant of c-Jun N-terminal kinase blocked the ability of VES to induce differentiation; however, treatment of cells with PD 98059, a chemical inhibitor of mitogen-activated protein kinase kinase (MEK1/2), blocked the ability of VES to induce differentiation.

Authors+Show Affiliations

School of Biological Sciences/C0900, University of Texas at Austin, Austin, Texas 78712, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11571230

Citation

You, H, et al. "RRR-alpha-tocopheryl Succinate Induces MDA-MB-435 and MCF-7 Human Breast Cancer Cells to Undergo Differentiation." Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research, vol. 12, no. 9, 2001, pp. 471-80.
You H, Yu W, Sanders BG, et al. RRR-alpha-tocopheryl succinate induces MDA-MB-435 and MCF-7 human breast cancer cells to undergo differentiation. Cell Growth Differ. 2001;12(9):471-80.
You, H., Yu, W., Sanders, B. G., & Kline, K. (2001). RRR-alpha-tocopheryl succinate induces MDA-MB-435 and MCF-7 human breast cancer cells to undergo differentiation. Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research, 12(9), pp. 471-80.
You H, et al. RRR-alpha-tocopheryl Succinate Induces MDA-MB-435 and MCF-7 Human Breast Cancer Cells to Undergo Differentiation. Cell Growth Differ. 2001;12(9):471-80. PubMed PMID: 11571230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RRR-alpha-tocopheryl succinate induces MDA-MB-435 and MCF-7 human breast cancer cells to undergo differentiation. AU - You,H, AU - Yu,W, AU - Sanders,B G, AU - Kline,K, PY - 2001/9/26/pubmed PY - 2002/1/5/medline PY - 2001/9/26/entrez SP - 471 EP - 80 JF - Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research JO - Cell Growth Differ. VL - 12 IS - 9 N2 - RRR-alpha-Tocopheryl succinate (vitamin E succinate, VES) is a potent antitumor agent, inducing DNA synthesis arrest, differentiation, and apoptosis. Because little is known about VES-induced differentiation, studies reported here characterize VES effects on the differentiation status of human breast cancer cell lines and investigate possible molecular mechanisms involved. VES-induced differentiation of human MCF-7 and MDA-MB-435 breast cancer cells was characterized by morphological changes, induction of lipid droplets, induction of beta-casein mRNA expression, and down-regulation of Her2/neu protein. In contrast, VES treatment of normal human mammary epithelial cells, MCF-10A cells, and T-47D cells did not induce differentiation. Studies addressing mechanisms showed that neither antibody neutralization of the transforming growth factor-beta signaling pathway nor expression of a dominant-negative mutant of c-Jun N-terminal kinase blocked the ability of VES to induce differentiation; however, treatment of cells with PD 98059, a chemical inhibitor of mitogen-activated protein kinase kinase (MEK1/2), blocked the ability of VES to induce differentiation. SN - 1044-9523 UR - https://www.unboundmedicine.com/medline/citation/11571230/RRR_alpha_tocopheryl_succinate_induces_MDA_MB_435_and_MCF_7_human_breast_cancer_cells_to_undergo_differentiation_ L2 - http://cgd.aacrjournals.org/cgi/pmidlookup?view=long&pmid=11571230 DB - PRIME DP - Unbound Medicine ER -