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Effect of thymine glycol on transcription elongation by T7 RNA polymerase and mammalian RNA polymerase II.
J Biol Chem. 2001 Nov 30; 276(48):45367-71.JB

Abstract

Thymine glycols are formed in DNA by exposure to ionizing radiation or oxidative stress. Although these lesions are repaired by the base excision repair pathway, they have been shown also to be subject to transcription-coupled repair. A current model for transcription-coupled repair proposes that RNA polymerase II arrested at a DNA lesion provides a signal for recruitment of the repair enzymes to the lesion site. Here we report the effect of thymine glycol on transcription elongation by T7 RNA polymerase and RNA polymerase II from rat liver. DNA substrates containing a single thymine glycol located either in the transcribed or nontranscribed strand were used to carry out in vitro transcription. We found that thymine glycol in the transcribed strand blocked transcription elongation by T7 RNA polymerase approximately 50% of the time but did not block RNA polymerase II. Thymine glycol in the nontranscribed strand did not affect transcription by either polymerase. These results suggest that arrest of RNA polymerase elongation by thymine glycol is not necessary for transcription-coupled repair of this lesion. Additional factors that recognize and bind thymine glycol in DNA may be required to ensure RNA polymerase arrest and the initiation of transcription-coupled repair in vivo.

Authors+Show Affiliations

Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11571287

Citation

Tornaletti, S, et al. "Effect of Thymine Glycol On Transcription Elongation By T7 RNA Polymerase and Mammalian RNA Polymerase II." The Journal of Biological Chemistry, vol. 276, no. 48, 2001, pp. 45367-71.
Tornaletti S, Maeda LS, Lloyd DR, et al. Effect of thymine glycol on transcription elongation by T7 RNA polymerase and mammalian RNA polymerase II. J Biol Chem. 2001;276(48):45367-71.
Tornaletti, S., Maeda, L. S., Lloyd, D. R., Reines, D., & Hanawalt, P. C. (2001). Effect of thymine glycol on transcription elongation by T7 RNA polymerase and mammalian RNA polymerase II. The Journal of Biological Chemistry, 276(48), 45367-71.
Tornaletti S, et al. Effect of Thymine Glycol On Transcription Elongation By T7 RNA Polymerase and Mammalian RNA Polymerase II. J Biol Chem. 2001 Nov 30;276(48):45367-71. PubMed PMID: 11571287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of thymine glycol on transcription elongation by T7 RNA polymerase and mammalian RNA polymerase II. AU - Tornaletti,S, AU - Maeda,L S, AU - Lloyd,D R, AU - Reines,D, AU - Hanawalt,P C, Y1 - 2001/09/24/ PY - 2001/9/26/pubmed PY - 2002/1/11/medline PY - 2001/9/26/entrez SP - 45367 EP - 71 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 276 IS - 48 N2 - Thymine glycols are formed in DNA by exposure to ionizing radiation or oxidative stress. Although these lesions are repaired by the base excision repair pathway, they have been shown also to be subject to transcription-coupled repair. A current model for transcription-coupled repair proposes that RNA polymerase II arrested at a DNA lesion provides a signal for recruitment of the repair enzymes to the lesion site. Here we report the effect of thymine glycol on transcription elongation by T7 RNA polymerase and RNA polymerase II from rat liver. DNA substrates containing a single thymine glycol located either in the transcribed or nontranscribed strand were used to carry out in vitro transcription. We found that thymine glycol in the transcribed strand blocked transcription elongation by T7 RNA polymerase approximately 50% of the time but did not block RNA polymerase II. Thymine glycol in the nontranscribed strand did not affect transcription by either polymerase. These results suggest that arrest of RNA polymerase elongation by thymine glycol is not necessary for transcription-coupled repair of this lesion. Additional factors that recognize and bind thymine glycol in DNA may be required to ensure RNA polymerase arrest and the initiation of transcription-coupled repair in vivo. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/11571287/Effect_of_thymine_glycol_on_transcription_elongation_by_T7_RNA_polymerase_and_mammalian_RNA_polymerase_II_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=11571287 DB - PRIME DP - Unbound Medicine ER -