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Solid-phase microextraction and gas chromatography-mass spectrometry analysis of p,p' -DDE in biological samples.
J Chromatogr B Biomed Sci Appl. 2001 Sep 15; 761(1):93-8.JC

Abstract

A simple and rapid extraction method for the analysis of p,p'-DDE from rat whole blood and tissues was developed using headspace solid-phase microextraction (SPME). A vial containing a sample of p,p'-DDE, sodium hydroxide, and benzophenone as internal standard was heated at 120 degrees C. A polydimethylsiloxane-coated SPME fiber was exposed for 15 min in the headspace of the vial. The fiber needle was then injected and desorbed by exposing the fiber in the injection port of a capillary gas chromatography-mass spectrometry system. The calibration curve demonstrated good linearity throughout the concentration range from 0.02 to 50 microg/g for rat whole blood and liver samples. The limit of detection for p,p'-DDE was 0.020 microg/g using 0.5 g rat whole blood and liver samples. Coefficients of variation ranged from 7.0 to 7.9%. This method was used to analyze a rat whole blood sample after administration of p,p'-DDE.

Authors+Show Affiliations

Department of Forensic Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11585136

Citation

Kusakabe, T, et al. "Solid-phase Microextraction and Gas Chromatography-mass Spectrometry Analysis of P,p' -DDE in Biological Samples." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 761, no. 1, 2001, pp. 93-8.
Kusakabe T, Saito T, Takeichi S. Solid-phase microextraction and gas chromatography-mass spectrometry analysis of p,p' -DDE in biological samples. J Chromatogr B Biomed Sci Appl. 2001;761(1):93-8.
Kusakabe, T., Saito, T., & Takeichi, S. (2001). Solid-phase microextraction and gas chromatography-mass spectrometry analysis of p,p' -DDE in biological samples. Journal of Chromatography. B, Biomedical Sciences and Applications, 761(1), 93-8.
Kusakabe T, Saito T, Takeichi S. Solid-phase Microextraction and Gas Chromatography-mass Spectrometry Analysis of P,p' -DDE in Biological Samples. J Chromatogr B Biomed Sci Appl. 2001 Sep 15;761(1):93-8. PubMed PMID: 11585136.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid-phase microextraction and gas chromatography-mass spectrometry analysis of p,p' -DDE in biological samples. AU - Kusakabe,T, AU - Saito,T, AU - Takeichi,S, PY - 2001/10/5/pubmed PY - 2002/3/23/medline PY - 2001/10/5/entrez SP - 93 EP - 8 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 761 IS - 1 N2 - A simple and rapid extraction method for the analysis of p,p'-DDE from rat whole blood and tissues was developed using headspace solid-phase microextraction (SPME). A vial containing a sample of p,p'-DDE, sodium hydroxide, and benzophenone as internal standard was heated at 120 degrees C. A polydimethylsiloxane-coated SPME fiber was exposed for 15 min in the headspace of the vial. The fiber needle was then injected and desorbed by exposing the fiber in the injection port of a capillary gas chromatography-mass spectrometry system. The calibration curve demonstrated good linearity throughout the concentration range from 0.02 to 50 microg/g for rat whole blood and liver samples. The limit of detection for p,p'-DDE was 0.020 microg/g using 0.5 g rat whole blood and liver samples. Coefficients of variation ranged from 7.0 to 7.9%. This method was used to analyze a rat whole blood sample after administration of p,p'-DDE. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/11585136/Solid_phase_microextraction_and_gas_chromatography_mass_spectrometry_analysis_of_pp'__DDE_in_biological_samples_ DB - PRIME DP - Unbound Medicine ER -