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Cooperative effects of exercise training and genistein administration on bone mass in ovariectomized mice.
J Bone Miner Res 2001; 16(10):1829-36JB

Abstract

We reported that genistein, a soybean isoflavone, prevents bone loss caused by estrogen deficiency, without undesirable effects on the uterus. In this study, we examined cooperative effects of genistein administration and running exercise on bone mass in ovariectomized (OVX) mice. Female mice aged 7 weeks were either sham-operated or OVX and divided into six groups: (1) sham; (2) OVX; (3) OVX, treated with genistein at a submaximal dose (0.4 mg/day) subcutaneously (G); (4) OVX, exercised on a treadmill daily for 30 minutes/day at 12 m/minute on a 10 degree uphill slope (Ex); (5) OVX, given genistein and exercised (ExG); and (6) OVX, treated with 17beta-estradiol (0.03 microg/day) in the same manner as genistein (E2). Four weeks after intervention, bone mass was estimated by dual-energy X-ray absorptiometry (DXA) and peripheral quantitative computed tomography (pQCT). Bone mineral density (BMD) of the whole femur measured by DXA was higher in both the G and the Ex groups than in the OVX group. Furthermore, BMD in the ExG group was significantly higher than that in the groups receiving either intervention alone. Bone area in distal region of the femur was significantly higher in Ex and ExG groups as compared with those in the OVX and G groups. pQCT analysis showed that the cross-sectional areas (CSAs) and periosteum perimeter at midshaft of the femur did not differ in the sham and OVX groups but were significantly higher in Ex and ExG groups. Histomorphometric analysis showed that bone formation rate/bone surface (BFR/BS) was significantly higher in both Ex and ExG groups as compared with that in non-exercised groups. The bone volume (BV/TV) in the distal femoral cancellous bone was lower in the OVX than that in the sham group, and it was restored completely in the ExG group, as in the E2 group. Thickness of the trabecular bone (Tb.Th) was higher in Ex and ExG groups than that in the OVX and G groups. These results indicate that the combined intervention of moderate exercise and the submaximal dose of genistein administration show a cooperative effect in preventing bone loss in OVX mice.

Authors+Show Affiliations

Division of Food Science, The National Institute of Health and Nutrition, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11585347

Citation

Wu, J, et al. "Cooperative Effects of Exercise Training and Genistein Administration On Bone Mass in Ovariectomized Mice." Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research, vol. 16, no. 10, 2001, pp. 1829-36.
Wu J, Wang XX, Takasaki M, et al. Cooperative effects of exercise training and genistein administration on bone mass in ovariectomized mice. J Bone Miner Res. 2001;16(10):1829-36.
Wu, J., Wang, X. X., Takasaki, M., Ohta, A., Higuchi, M., & Ishimi, Y. (2001). Cooperative effects of exercise training and genistein administration on bone mass in ovariectomized mice. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research, 16(10), pp. 1829-36.
Wu J, et al. Cooperative Effects of Exercise Training and Genistein Administration On Bone Mass in Ovariectomized Mice. J Bone Miner Res. 2001;16(10):1829-36. PubMed PMID: 11585347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cooperative effects of exercise training and genistein administration on bone mass in ovariectomized mice. AU - Wu,J, AU - Wang,X X, AU - Takasaki,M, AU - Ohta,A, AU - Higuchi,M, AU - Ishimi,Y, PY - 2001/10/5/pubmed PY - 2002/2/28/medline PY - 2001/10/5/entrez SP - 1829 EP - 36 JF - Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research JO - J. Bone Miner. Res. VL - 16 IS - 10 N2 - We reported that genistein, a soybean isoflavone, prevents bone loss caused by estrogen deficiency, without undesirable effects on the uterus. In this study, we examined cooperative effects of genistein administration and running exercise on bone mass in ovariectomized (OVX) mice. Female mice aged 7 weeks were either sham-operated or OVX and divided into six groups: (1) sham; (2) OVX; (3) OVX, treated with genistein at a submaximal dose (0.4 mg/day) subcutaneously (G); (4) OVX, exercised on a treadmill daily for 30 minutes/day at 12 m/minute on a 10 degree uphill slope (Ex); (5) OVX, given genistein and exercised (ExG); and (6) OVX, treated with 17beta-estradiol (0.03 microg/day) in the same manner as genistein (E2). Four weeks after intervention, bone mass was estimated by dual-energy X-ray absorptiometry (DXA) and peripheral quantitative computed tomography (pQCT). Bone mineral density (BMD) of the whole femur measured by DXA was higher in both the G and the Ex groups than in the OVX group. Furthermore, BMD in the ExG group was significantly higher than that in the groups receiving either intervention alone. Bone area in distal region of the femur was significantly higher in Ex and ExG groups as compared with those in the OVX and G groups. pQCT analysis showed that the cross-sectional areas (CSAs) and periosteum perimeter at midshaft of the femur did not differ in the sham and OVX groups but were significantly higher in Ex and ExG groups. Histomorphometric analysis showed that bone formation rate/bone surface (BFR/BS) was significantly higher in both Ex and ExG groups as compared with that in non-exercised groups. The bone volume (BV/TV) in the distal femoral cancellous bone was lower in the OVX than that in the sham group, and it was restored completely in the ExG group, as in the E2 group. Thickness of the trabecular bone (Tb.Th) was higher in Ex and ExG groups than that in the OVX and G groups. These results indicate that the combined intervention of moderate exercise and the submaximal dose of genistein administration show a cooperative effect in preventing bone loss in OVX mice. SN - 0884-0431 UR - https://www.unboundmedicine.com/medline/citation/11585347/Cooperative_effects_of_exercise_training_and_genistein_administration_on_bone_mass_in_ovariectomized_mice_ L2 - https://doi.org/10.1359/jbmr.2001.16.10.1829 DB - PRIME DP - Unbound Medicine ER -