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Quality control of mRNA 3'-end processing is linked to the nuclear exosome.
Nature. 2001 Oct 04; 413(6855):538-42.Nat

Abstract

An emerging theme in messenger RNA metabolism is the coupling of nuclear pre-mRNA processing events, which contributes to mRNA quality control. Most eukaryotic mRNAs acquire a poly(A) tail during 3'-end processing within the nucleus, and this is coupled to efficient export of mRNAs to the cytoplasm. In the yeast Saccharomyces cerevisiae, a common consequence of defective nuclear export of mRNA is the hyperadenylation of nascent transcripts, which are sequestered at or near their sites of transcription. This implies that polyadenylation and nuclear export are coupled in a step that involves the release of mRNA from transcription site foci. Here we demonstrate that transcripts which fail to acquire a poly(A) tail are also retained at or near transcription sites. Surprisingly, this retention mechanism requires the protein Rrp6p and the nuclear exosome, a large complex of exonucleolytic enzymes. In exosome mutants, hypo- as well as hyperadenylated mRNAs are released and translated. These observations suggest that the exosome contributes to a checkpoint that monitors proper 3'-end formation of mRNA.

Authors+Show Affiliations

Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA. hilleren@u.arizona.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11586364

Citation

Hilleren, P, et al. "Quality Control of mRNA 3'-end Processing Is Linked to the Nuclear Exosome." Nature, vol. 413, no. 6855, 2001, pp. 538-42.
Hilleren P, McCarthy T, Rosbash M, et al. Quality control of mRNA 3'-end processing is linked to the nuclear exosome. Nature. 2001;413(6855):538-42.
Hilleren, P., McCarthy, T., Rosbash, M., Parker, R., & Jensen, T. H. (2001). Quality control of mRNA 3'-end processing is linked to the nuclear exosome. Nature, 413(6855), 538-42.
Hilleren P, et al. Quality Control of mRNA 3'-end Processing Is Linked to the Nuclear Exosome. Nature. 2001 Oct 4;413(6855):538-42. PubMed PMID: 11586364.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quality control of mRNA 3'-end processing is linked to the nuclear exosome. AU - Hilleren,P, AU - McCarthy,T, AU - Rosbash,M, AU - Parker,R, AU - Jensen,T H, PY - 2001/10/5/pubmed PY - 2001/11/3/medline PY - 2001/10/5/entrez SP - 538 EP - 42 JF - Nature JO - Nature VL - 413 IS - 6855 N2 - An emerging theme in messenger RNA metabolism is the coupling of nuclear pre-mRNA processing events, which contributes to mRNA quality control. Most eukaryotic mRNAs acquire a poly(A) tail during 3'-end processing within the nucleus, and this is coupled to efficient export of mRNAs to the cytoplasm. In the yeast Saccharomyces cerevisiae, a common consequence of defective nuclear export of mRNA is the hyperadenylation of nascent transcripts, which are sequestered at or near their sites of transcription. This implies that polyadenylation and nuclear export are coupled in a step that involves the release of mRNA from transcription site foci. Here we demonstrate that transcripts which fail to acquire a poly(A) tail are also retained at or near transcription sites. Surprisingly, this retention mechanism requires the protein Rrp6p and the nuclear exosome, a large complex of exonucleolytic enzymes. In exosome mutants, hypo- as well as hyperadenylated mRNAs are released and translated. These observations suggest that the exosome contributes to a checkpoint that monitors proper 3'-end formation of mRNA. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/11586364/Quality_control_of_mRNA_3'_end_processing_is_linked_to_the_nuclear_exosome_ L2 - https://doi.org/10.1038/35097110 DB - PRIME DP - Unbound Medicine ER -