Tags

Type your tag names separated by a space and hit enter

Kiwifruit protects against oxidative DNA damage in human cells and in vitro.
Nutr Cancer. 2001; 39(1):148-53.NC

Abstract

Antioxidant micronutrients may account for the beneficial effects of fruits on human health. A direct demonstration that consumption of fruit decreases oxidative DNA damage in human cells would support this hypothesis. Kiwifruit was taken as an example of a food with putative antioxidant properties, and its effectiveness at decreasing oxidative DNA damage was assessed in ex vivo as well as in vitro tests. The comet assay (single-cell gel electrophoresis) was used to measure DNA damage in lymphocytes collected during a human supplementation trial with a single 0.5-liter drink of kiwifruit juice (with water as a control). The comet assay was also modified to assess the antioxidant effect of kiwifruit in vitro by measuring the ability of an extract to interfere with oxidative damage to DNA induced by H2O2. Ex vivo, consumption of kiwifruit led to an increased resistance of DNA to oxidative damage induced by H2O2 in isolated lymphocytes, in comparison with lymphocytes collected after a control drink of water. No effect was seen on endogenous DNA damage. In vitro, a simple extract of kiwifruit, buffered to pH 7, was more effective than a solution of vitamin C (of equivalent concentration) at protecting DNA from damage, whereas at the highest concentrations tested, neither kiwi extract nor vitamin C had a protective effect. We have demonstrated significant antioxidant activity of kiwifruit ex vivo and in vitro, not attributable entirely to the vitamin C content of the fruit. Our dual approach is appropriate for testing other fruit and vegetable products for potential antioxidant effects.

Links

Publisher Full Text

Authors+Show Affiliations

Collins BH
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK.
Horská A
No affiliation info available
Hotten PM
No affiliation info available
Riddoch C
No affiliation info available
Collins AR
No affiliation info available

MeSH

ActinidiaAdultAntioxidantsAscorbic AcidCells, CulturedComet AssayDNADNA DamageFemaleFruitHumansLymphocytesMaleMiddle AgedOxidation-ReductionOxidative StressPlant Extracts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11588897

Citation

Collins, B H., et al. "Kiwifruit Protects Against Oxidative DNA Damage in Human Cells and in Vitro." Nutrition and Cancer, vol. 39, no. 1, 2001, pp. 148-53.
Collins BH, Horská A, Hotten PM, et al. Kiwifruit protects against oxidative DNA damage in human cells and in vitro. Nutr Cancer. 2001;39(1):148-53.
Collins, B. H., Horská, A., Hotten, P. M., Riddoch, C., & Collins, A. R. (2001). Kiwifruit protects against oxidative DNA damage in human cells and in vitro. Nutrition and Cancer, 39(1), 148-53.
Collins BH, et al. Kiwifruit Protects Against Oxidative DNA Damage in Human Cells and in Vitro. Nutr Cancer. 2001;39(1):148-53. PubMed PMID: 11588897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kiwifruit protects against oxidative DNA damage in human cells and in vitro. AU - Collins,B H, AU - Horská,A, AU - Hotten,P M, AU - Riddoch,C, AU - Collins,A R, PY - 2001/10/9/pubmed PY - 2002/5/10/medline PY - 2001/10/9/entrez SP - 148 EP - 53 JF - Nutrition and cancer JO - Nutr Cancer VL - 39 IS - 1 N2 - Antioxidant micronutrients may account for the beneficial effects of fruits on human health. A direct demonstration that consumption of fruit decreases oxidative DNA damage in human cells would support this hypothesis. Kiwifruit was taken as an example of a food with putative antioxidant properties, and its effectiveness at decreasing oxidative DNA damage was assessed in ex vivo as well as in vitro tests. The comet assay (single-cell gel electrophoresis) was used to measure DNA damage in lymphocytes collected during a human supplementation trial with a single 0.5-liter drink of kiwifruit juice (with water as a control). The comet assay was also modified to assess the antioxidant effect of kiwifruit in vitro by measuring the ability of an extract to interfere with oxidative damage to DNA induced by H2O2. Ex vivo, consumption of kiwifruit led to an increased resistance of DNA to oxidative damage induced by H2O2 in isolated lymphocytes, in comparison with lymphocytes collected after a control drink of water. No effect was seen on endogenous DNA damage. In vitro, a simple extract of kiwifruit, buffered to pH 7, was more effective than a solution of vitamin C (of equivalent concentration) at protecting DNA from damage, whereas at the highest concentrations tested, neither kiwi extract nor vitamin C had a protective effect. We have demonstrated significant antioxidant activity of kiwifruit ex vivo and in vitro, not attributable entirely to the vitamin C content of the fruit. Our dual approach is appropriate for testing other fruit and vegetable products for potential antioxidant effects. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/11588897/Kiwifruit_protects_against_oxidative_DNA_damage_in_human_cells_and_in_vitro_ L2 - https://www.tandfonline.com/doi/full/10.1207/S15327914nc391_20 DB - PRIME DP - Unbound Medicine ER -