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On-line capillary electrophoresis-electrospray mass spectrometry for the stereoselective analysis of drugs and metabolites.
Electrophoresis. 2001 Sep; 22(15):3308-15.E

Abstract

The on-line combination of partial-filling capillary electrophoresis and electrospray ionization mass spectrometry was demonstrated for the enantioseparation of pharmaceutical drugs and metabolites, namely amphetamines, methadone, venlafaxine and selected tropane alkaloids. The partial-filling technique proved to be a suitable and efficient approach to avoid mass spectrometry (MS) source contamination, as well as signal suppression due to nonvolatile additives. To achieve chiral separation, various chiral selectors were applied, including neutral and particularly negatively charged cyclodextrins. Because of the countercurrent contribution, charged cyclodextrins were found more suitable for the on-line MS detection of separated enantiomers. Hyphenation of capillary electrophoresis (CE) with mass spectrometry was found appropriate for the stereoselective analysis of methadone in real serum samples. Moreover, the use of MS in the selected ion monitoring mode resulted in a very high selectivity, as well as improved sensitivity compared to UV detection. Finally, with atropine as a model compound, the quantitative performances of the method were evaluated and showed high sensitivity, as well as good repeatability in terms of migration time and peak area ratio.

Authors+Show Affiliations

Cherkaoui S
Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Switzerland.
Rudaz S
No affiliation info available
Varesio E
No affiliation info available
Veuthey JL
No affiliation info available

MeSH

AmphetaminesCyclodextrinsCyclohexanolsElectrophoresis, CapillaryIndicators and ReagentsMethadonePharmaceutical PreparationsSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationTropanesVenlafaxine Hydrochloride

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

11589295

Citation

Cherkaoui, S, et al. "On-line Capillary Electrophoresis-electrospray Mass Spectrometry for the Stereoselective Analysis of Drugs and Metabolites." Electrophoresis, vol. 22, no. 15, 2001, pp. 3308-15.
Cherkaoui S, Rudaz S, Varesio E, et al. On-line capillary electrophoresis-electrospray mass spectrometry for the stereoselective analysis of drugs and metabolites. Electrophoresis. 2001;22(15):3308-15.
Cherkaoui, S., Rudaz, S., Varesio, E., & Veuthey, J. L. (2001). On-line capillary electrophoresis-electrospray mass spectrometry for the stereoselective analysis of drugs and metabolites. Electrophoresis, 22(15), 3308-15.
Cherkaoui S, et al. On-line Capillary Electrophoresis-electrospray Mass Spectrometry for the Stereoselective Analysis of Drugs and Metabolites. Electrophoresis. 2001;22(15):3308-15. PubMed PMID: 11589295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line capillary electrophoresis-electrospray mass spectrometry for the stereoselective analysis of drugs and metabolites. AU - Cherkaoui,S, AU - Rudaz,S, AU - Varesio,E, AU - Veuthey,J L, PY - 2001/10/9/pubmed PY - 2002/2/22/medline PY - 2001/10/9/entrez SP - 3308 EP - 15 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 15 N2 - The on-line combination of partial-filling capillary electrophoresis and electrospray ionization mass spectrometry was demonstrated for the enantioseparation of pharmaceutical drugs and metabolites, namely amphetamines, methadone, venlafaxine and selected tropane alkaloids. The partial-filling technique proved to be a suitable and efficient approach to avoid mass spectrometry (MS) source contamination, as well as signal suppression due to nonvolatile additives. To achieve chiral separation, various chiral selectors were applied, including neutral and particularly negatively charged cyclodextrins. Because of the countercurrent contribution, charged cyclodextrins were found more suitable for the on-line MS detection of separated enantiomers. Hyphenation of capillary electrophoresis (CE) with mass spectrometry was found appropriate for the stereoselective analysis of methadone in real serum samples. Moreover, the use of MS in the selected ion monitoring mode resulted in a very high selectivity, as well as improved sensitivity compared to UV detection. Finally, with atropine as a model compound, the quantitative performances of the method were evaluated and showed high sensitivity, as well as good repeatability in terms of migration time and peak area ratio. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11589295/On_line_capillary_electrophoresis_electrospray_mass_spectrometry_for_the_stereoselective_analysis_of_drugs_and_metabolites_ L2 - https://doi.org/10.1002/1522-2683(200109)22:15<3308::AID-ELPS3308>3.0.CO;2-H DB - PRIME DP - Unbound Medicine ER -