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[The study of PAI-1 promotor region gene polymorphism in cerebrovascular disease].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001 Oct; 18(5):383-7.ZY

Abstract

OBJECTIVE

To investigate the relationship between the plasminogen activator inhibitor-1(PAI-1) gene polymorphism and cerebrovascular disease and detect whether it plays an important role in the pathogenesis of cerebrovascular disease.

METHODS

Peripheral blood leukocytes samples were collected from 60 normal controls, 65 patients with acute cerebral infarction (CI) and 31 hypertensives complicated with cerebral hemorrhage. The 4G/5G allele polymorphism in the PAI-1 gene promotor region of the leukocytes was genotyped by polymerase chain reaction. The plasma PAI-1 activity was assayed by ELISA.

RESULTS

The plasma PAI-1 activity level in the CI group was significantly higher than those in the other two groups. PAI-1 level in 4G allele homozygous genotype was significantly higher than the PAI-1 levels in 4G/5G heterozygous and 5G homozygous. Although PAI-1 level was higher in 4G/5G heterozygous genotype than in 5G homozygous, the difference was not statistically significant. There were significant differences between acute cerebral infarction PAI-1 gene polymorphism and controls (P<0.05), and the frequencies of 4G/4G genotype in patients with acute cerebral infarction (43.08%) were higher than those in normal controls (20.00%, P<0.05). There were no significant differences between hypertensives complicated with hemorrhage (25.81%) and controls (P>0.05), and the PAI-1 levels of the 4G/4G genotype in the female patients with CI were higher than those in the male patients with CI of the same genotype.

CONCLUSION

The results suggest that PAI-1 gene polymorphism may be a susceptible factor to acute cerebral infarction in Chinese, and 4G allele homozygous genotype may be the major risk factor for acute cerebral infarction, and it may be especially an independent risk factor of cerebral infarction in female patients.

Authors+Show Affiliations

Zhang C
Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, 266003 P.R. China.
Li J
No affiliation info available
Li L
No affiliation info available
Luo B
No affiliation info available

MeSH

Cerebral HemorrhageCerebral InfarctionFemaleHumansMalePlasminogen Activator Inhibitor 1Polymorphism, GeneticPromoter Regions, GeneticSex Factors

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11592048

Citation

Zhang, C, et al. "[The Study of PAI-1 Promotor Region Gene Polymorphism in Cerebrovascular Disease]." Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, vol. 18, no. 5, 2001, pp. 383-7.
Zhang C, Li J, Li L, et al. [The study of PAI-1 promotor region gene polymorphism in cerebrovascular disease]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001;18(5):383-7.
Zhang, C., Li, J., Li, L., & Luo, B. (2001). [The study of PAI-1 promotor region gene polymorphism in cerebrovascular disease]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, 18(5), 383-7.
Zhang C, et al. [The Study of PAI-1 Promotor Region Gene Polymorphism in Cerebrovascular Disease]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001;18(5):383-7. PubMed PMID: 11592048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The study of PAI-1 promotor region gene polymorphism in cerebrovascular disease]. AU - Zhang,C, AU - Li,J, AU - Li,L, AU - Luo,B, PY - 2001/10/10/pubmed PY - 2002/1/5/medline PY - 2001/10/10/entrez SP - 383 EP - 7 JF - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics JO - Zhonghua Yi Xue Yi Chuan Xue Za Zhi VL - 18 IS - 5 N2 - OBJECTIVE: To investigate the relationship between the plasminogen activator inhibitor-1(PAI-1) gene polymorphism and cerebrovascular disease and detect whether it plays an important role in the pathogenesis of cerebrovascular disease. METHODS: Peripheral blood leukocytes samples were collected from 60 normal controls, 65 patients with acute cerebral infarction (CI) and 31 hypertensives complicated with cerebral hemorrhage. The 4G/5G allele polymorphism in the PAI-1 gene promotor region of the leukocytes was genotyped by polymerase chain reaction. The plasma PAI-1 activity was assayed by ELISA. RESULTS: The plasma PAI-1 activity level in the CI group was significantly higher than those in the other two groups. PAI-1 level in 4G allele homozygous genotype was significantly higher than the PAI-1 levels in 4G/5G heterozygous and 5G homozygous. Although PAI-1 level was higher in 4G/5G heterozygous genotype than in 5G homozygous, the difference was not statistically significant. There were significant differences between acute cerebral infarction PAI-1 gene polymorphism and controls (P<0.05), and the frequencies of 4G/4G genotype in patients with acute cerebral infarction (43.08%) were higher than those in normal controls (20.00%, P<0.05). There were no significant differences between hypertensives complicated with hemorrhage (25.81%) and controls (P>0.05), and the PAI-1 levels of the 4G/4G genotype in the female patients with CI were higher than those in the male patients with CI of the same genotype. CONCLUSION: The results suggest that PAI-1 gene polymorphism may be a susceptible factor to acute cerebral infarction in Chinese, and 4G allele homozygous genotype may be the major risk factor for acute cerebral infarction, and it may be especially an independent risk factor of cerebral infarction in female patients. SN - 1003-9406 UR - https://www.unboundmedicine.com/medline/citation/11592048/[The_study_of_PAI_1_promotor_region_gene_polymorphism_in_cerebrovascular_disease]_ DB - PRIME DP - Unbound Medicine ER -