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Hypertension and impairment of endothelium-dependent relaxation of arteries from spontaneously hypertensive and L-NAME-treated Wistar rats.
J Smooth Muscle Res. 2001 Apr; 37(2):67-79.JS

Abstract

Effects of chronic treatment of normotensive Wistar rats with N(omega)-nitro-L-arginine methyl ester (L-NAME) on blood pressure and on endothelium-dependent relaxation of the aorta, carotid and iliac arteries were studied. The endothelium-dependent relaxation was compared in arteries from normotensive Wistar Kyoto rats (WKY) and genetically hypertensive rats (stroke-prone spontaneously hypertensive rats, SHRSP). Chronic treatment of normotensive Wistar rats with L-NAME caused an elevation of blood pressure. The elevated blood pressure at 15 weeks of age was significantly higher in these animals than that of untreated Wistar rats, but lower than that of SHRSP. Endothelium-dependent relaxation of the arteries induced by acetylcholine (ACh) was almost abolished by chronic treatment with L-NAME. The remaining small relaxation in arteries from L-NAME-treated rats was completely inhibited by application of L-NAME (10(-4) M). In such preparations, higher concentrations of ACh induced a contraction, which was abolished by removal of the endothelium or by an application of indomethacin (10(-5) M). Endothelium-independent relaxation induced by sodium nitroprusside was similar between preparations from untreated and L-NAME-treated Wistar rats. Endothelium-dependent relaxation was significantly impaired in preparations from SHRSP, when compared with that in those from WKY. However, the impairment was less prominent in preparations from SHRSP than in those from L-NAME-treated rats. These results suggest that the impairment of endothelium-dependent relaxation in the arteries from L-NAME-treated rats is not due to the elevated blood pressure resulting from the chronic treatment, and that impairment of NO synthesis by the endothelium does not play a major role in the initiation of hypertension in SHRSP.

Authors+Show Affiliations

Department of Anatomy and Physiology, Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Osaka, Japan. fumiko@phar.kindai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11592285

Citation

Sekiguchi, F, et al. "Hypertension and Impairment of Endothelium-dependent Relaxation of Arteries From Spontaneously Hypertensive and L-NAME-treated Wistar Rats." Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi, vol. 37, no. 2, 2001, pp. 67-79.
Sekiguchi F, Miyake Y, Hirakawa A, et al. Hypertension and impairment of endothelium-dependent relaxation of arteries from spontaneously hypertensive and L-NAME-treated Wistar rats. J Smooth Muscle Res. 2001;37(2):67-79.
Sekiguchi, F., Miyake, Y., Hirakawa, A., Nakahira, T., Yamaoka, M., Shimamura, K., Yamamoto, K., & Sunano, S. (2001). Hypertension and impairment of endothelium-dependent relaxation of arteries from spontaneously hypertensive and L-NAME-treated Wistar rats. Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi, 37(2), 67-79.
Sekiguchi F, et al. Hypertension and Impairment of Endothelium-dependent Relaxation of Arteries From Spontaneously Hypertensive and L-NAME-treated Wistar Rats. J Smooth Muscle Res. 2001;37(2):67-79. PubMed PMID: 11592285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypertension and impairment of endothelium-dependent relaxation of arteries from spontaneously hypertensive and L-NAME-treated Wistar rats. AU - Sekiguchi,F, AU - Miyake,Y, AU - Hirakawa,A, AU - Nakahira,T, AU - Yamaoka,M, AU - Shimamura,K, AU - Yamamoto,K, AU - Sunano,S, PY - 2001/10/11/pubmed PY - 2002/3/2/medline PY - 2001/10/11/entrez SP - 67 EP - 79 JF - Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi JO - J Smooth Muscle Res VL - 37 IS - 2 N2 - Effects of chronic treatment of normotensive Wistar rats with N(omega)-nitro-L-arginine methyl ester (L-NAME) on blood pressure and on endothelium-dependent relaxation of the aorta, carotid and iliac arteries were studied. The endothelium-dependent relaxation was compared in arteries from normotensive Wistar Kyoto rats (WKY) and genetically hypertensive rats (stroke-prone spontaneously hypertensive rats, SHRSP). Chronic treatment of normotensive Wistar rats with L-NAME caused an elevation of blood pressure. The elevated blood pressure at 15 weeks of age was significantly higher in these animals than that of untreated Wistar rats, but lower than that of SHRSP. Endothelium-dependent relaxation of the arteries induced by acetylcholine (ACh) was almost abolished by chronic treatment with L-NAME. The remaining small relaxation in arteries from L-NAME-treated rats was completely inhibited by application of L-NAME (10(-4) M). In such preparations, higher concentrations of ACh induced a contraction, which was abolished by removal of the endothelium or by an application of indomethacin (10(-5) M). Endothelium-independent relaxation induced by sodium nitroprusside was similar between preparations from untreated and L-NAME-treated Wistar rats. Endothelium-dependent relaxation was significantly impaired in preparations from SHRSP, when compared with that in those from WKY. However, the impairment was less prominent in preparations from SHRSP than in those from L-NAME-treated rats. These results suggest that the impairment of endothelium-dependent relaxation in the arteries from L-NAME-treated rats is not due to the elevated blood pressure resulting from the chronic treatment, and that impairment of NO synthesis by the endothelium does not play a major role in the initiation of hypertension in SHRSP. SN - 0916-8737 UR - https://www.unboundmedicine.com/medline/citation/11592285/Hypertension_and_impairment_of_endothelium_dependent_relaxation_of_arteries_from_spontaneously_hypertensive_and_L_NAME_treated_Wistar_rats_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/jsmr/37.67?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -