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Identification of human cancer-related genes by naturally occurring Hepatitis B Virus DNA tagging.
Oncogene. 2001 Sep 27; 20(43):6233-40.O

Abstract

Proviral tagging has been used in animals as a powerful tool for cancer genetics. We show that a similar approach is possible in patients with hepatocellular carcinoma (HCC) infected by Hepatitis B Virus (HBV), a human pararetrovirus which may act by insertional mutagenesis. In this work, the HBV genome is used as a probe to identify cancer-related genes. By using HBV-Alu-PCR, we obtained 21 HBV/cellular DNA junctions from 18 different patients. In six of 21, we found the HBV DNA integrated into a cellular gene: (1) Sarco/Endoplasmic Reticulum Calcium ATPase1 Gene; (2) Thyroid Hormone Receptor Associated Protein 150 alpha Gene; (3) Human Telomerase Reverse Transcriptase Gene; (4) Minichromosome Maintenance Protein (MCM)-Related Gene; (5) FR7, a new gene expressed in human liver and cancer tissues; and (6) Nuclear Matrix Protein p84 Gene. Seven junctions contained unique cellular sequences. In the remaining eight, the HBV DNA was next to repetitive sequences, five of them of LINE1 type. The cellular genes targeted by HBV are key regulators of cell proliferation and viability. Our results show that studies on HBV-related HCCs allow to identify cellular genes involved in cancer. We therefore propose this approach as a valuable tool for functional cancer genomic studies in humans.

Authors+Show Affiliations

U370 INSERM, Necker Institute, 75015, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11593432

Citation

Gozuacik, D, et al. "Identification of Human Cancer-related Genes By Naturally Occurring Hepatitis B Virus DNA Tagging." Oncogene, vol. 20, no. 43, 2001, pp. 6233-40.
Gozuacik D, Murakami Y, Saigo K, et al. Identification of human cancer-related genes by naturally occurring Hepatitis B Virus DNA tagging. Oncogene. 2001;20(43):6233-40.
Gozuacik, D., Murakami, Y., Saigo, K., Chami, M., Mugnier, C., Lagorce, D., Okanoue, T., Urashima, T., Bréchot, C., & Paterlini-Bréchot, P. (2001). Identification of human cancer-related genes by naturally occurring Hepatitis B Virus DNA tagging. Oncogene, 20(43), 6233-40.
Gozuacik D, et al. Identification of Human Cancer-related Genes By Naturally Occurring Hepatitis B Virus DNA Tagging. Oncogene. 2001 Sep 27;20(43):6233-40. PubMed PMID: 11593432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of human cancer-related genes by naturally occurring Hepatitis B Virus DNA tagging. AU - Gozuacik,D, AU - Murakami,Y, AU - Saigo,K, AU - Chami,M, AU - Mugnier,C, AU - Lagorce,D, AU - Okanoue,T, AU - Urashima,T, AU - Bréchot,C, AU - Paterlini-Bréchot,P, PY - 2001/01/15/received PY - 2001/07/05/revised PY - 2001/07/16/accepted PY - 2001/10/11/pubmed PY - 2001/11/3/medline PY - 2001/10/11/entrez SP - 6233 EP - 40 JF - Oncogene JO - Oncogene VL - 20 IS - 43 N2 - Proviral tagging has been used in animals as a powerful tool for cancer genetics. We show that a similar approach is possible in patients with hepatocellular carcinoma (HCC) infected by Hepatitis B Virus (HBV), a human pararetrovirus which may act by insertional mutagenesis. In this work, the HBV genome is used as a probe to identify cancer-related genes. By using HBV-Alu-PCR, we obtained 21 HBV/cellular DNA junctions from 18 different patients. In six of 21, we found the HBV DNA integrated into a cellular gene: (1) Sarco/Endoplasmic Reticulum Calcium ATPase1 Gene; (2) Thyroid Hormone Receptor Associated Protein 150 alpha Gene; (3) Human Telomerase Reverse Transcriptase Gene; (4) Minichromosome Maintenance Protein (MCM)-Related Gene; (5) FR7, a new gene expressed in human liver and cancer tissues; and (6) Nuclear Matrix Protein p84 Gene. Seven junctions contained unique cellular sequences. In the remaining eight, the HBV DNA was next to repetitive sequences, five of them of LINE1 type. The cellular genes targeted by HBV are key regulators of cell proliferation and viability. Our results show that studies on HBV-related HCCs allow to identify cellular genes involved in cancer. We therefore propose this approach as a valuable tool for functional cancer genomic studies in humans. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/11593432/Identification_of_human_cancer_related_genes_by_naturally_occurring_Hepatitis_B_Virus_DNA_tagging_ L2 - https://doi.org/10.1038/sj.onc.1204835 DB - PRIME DP - Unbound Medicine ER -