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Association of polymorphism of apolipoprotein E gene with coronary heart disease in Han Chinese.
Chin Med J (Engl). 1999 Mar; 112(3):224-7.CM

Abstract

OBJECTIVE

To investigate the association between apolipoprotein E (apoE) gene polymorphism and coronary heart disease(CHD) in Han Chinese.

METHODS

Apo E genotype was examined with the methods of hot start polymerase chain reaction (PCR) and restriction isotyping in samples of 113 unrelated Chinese healthy individuals and 93 patients with CHD. The relation of the gene polymorphism of apoE and levels of serum lipids, lipoproteins, and apolipoproteins was also studied.

RESULTS

The results showed that the epsilon 4/3 genotype was more frequent in CHD cases than in control subjects (31.2% vs. 11.5%, P < 0.01). The frequency of the epsilon 4 allele in CHD cases was significantly higher than in control subjects (17.2% vs 7.5%, P < 0.01). The epsilon 4 allele was associated with high concentrations of serum TC (r = 0.265, P < 0.05), LDL-C (r = 0.266, P < 0.05), and apoB (r = 0.360, P < 0.01).

CONCLUSIONS

Hot start PCR assay is considered a rapid and simple technique for apoE genotyping. This method is suitable for routine laboratories and large scale population studies. Genetic polymorphism of the apoE gene might contribute to the determination of serum lipid profile and the development of CHD among Han Chinese.

Authors+Show Affiliations

Department of Clinical Biochemistry, Peking Union Medical College Hospital, CAMS and PUMC, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11593554

Citation

Yan, S, et al. "Association of Polymorphism of Apolipoprotein E Gene With Coronary Heart Disease in Han Chinese." Chinese Medical Journal, vol. 112, no. 3, 1999, pp. 224-7.
Yan S, Zhou X, Lin Q, et al. Association of polymorphism of apolipoprotein E gene with coronary heart disease in Han Chinese. Chin Med J (Engl). 1999;112(3):224-7.
Yan, S., Zhou, X., Lin, Q., & Song, Y. (1999). Association of polymorphism of apolipoprotein E gene with coronary heart disease in Han Chinese. Chinese Medical Journal, 112(3), 224-7.
Yan S, et al. Association of Polymorphism of Apolipoprotein E Gene With Coronary Heart Disease in Han Chinese. Chin Med J (Engl). 1999;112(3):224-7. PubMed PMID: 11593554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of polymorphism of apolipoprotein E gene with coronary heart disease in Han Chinese. AU - Yan,S, AU - Zhou,X, AU - Lin,Q, AU - Song,Y, PY - 2001/10/12/pubmed PY - 2002/1/5/medline PY - 2001/10/12/entrez SP - 224 EP - 7 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 112 IS - 3 N2 - OBJECTIVE: To investigate the association between apolipoprotein E (apoE) gene polymorphism and coronary heart disease(CHD) in Han Chinese. METHODS: Apo E genotype was examined with the methods of hot start polymerase chain reaction (PCR) and restriction isotyping in samples of 113 unrelated Chinese healthy individuals and 93 patients with CHD. The relation of the gene polymorphism of apoE and levels of serum lipids, lipoproteins, and apolipoproteins was also studied. RESULTS: The results showed that the epsilon 4/3 genotype was more frequent in CHD cases than in control subjects (31.2% vs. 11.5%, P < 0.01). The frequency of the epsilon 4 allele in CHD cases was significantly higher than in control subjects (17.2% vs 7.5%, P < 0.01). The epsilon 4 allele was associated with high concentrations of serum TC (r = 0.265, P < 0.05), LDL-C (r = 0.266, P < 0.05), and apoB (r = 0.360, P < 0.01). CONCLUSIONS: Hot start PCR assay is considered a rapid and simple technique for apoE genotyping. This method is suitable for routine laboratories and large scale population studies. Genetic polymorphism of the apoE gene might contribute to the determination of serum lipid profile and the development of CHD among Han Chinese. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/11593554/Association_of_polymorphism_of_apolipoprotein_E_gene_with_coronary_heart_disease_in_Han_Chinese_ L2 - http://www.diseaseinfosearch.org/result/130 DB - PRIME DP - Unbound Medicine ER -