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Elevated expression of hTERT is associated with dysplastic cell transformation during human oral carcinogenesis in situ.
Clin Cancer Res. 2001 Oct; 7(10):3079-86.CC

Abstract

PURPOSE

Telomerase is a ribonucleoprotein complex composed of the catalytic protein subunit (human telomerase reverse transcriptase or hTERT) and the RNA template. This enzyme activity is a necessary and rate-limiting step of cellular immortalization and could provide a unique marker of aberrant cells, which may selectively be targeted. The current study was undertaken to quantitatively determine the degree of telomerase activation during multistage oral carcinogenesis using paraffin-embedded tissue samples.

EXPERIMENTAL DESIGN

hTERT expression level was quantitatively compared between normal and cancerous oral tissues by real-time reverse-transcription-PCR (RT-PCR). Also, the presence of hTERT transcript in individual cells was surveyed in the biopsy specimens with varying degrees of histopathology by in situ RT-PCR.

RESULTS

Low level of hTERT amplification was detected by real-time RT-PCR in most (11/13) normal human oral mucosa. hTERT expression was also detected in the majority (11/12) of squamous cell carcinoma tissues, and the level was significantly (P < 0.05) elevated, on the average, by a factor >6.9. By in situ RT-PCR, hTERT expression was not noted in normal epithelium (0/10) nor in mild dysplasia (0/7) but was detected in moderate dysplasia (4/5) and in those tissues with a higher grade of histopathology: severe dysplasia (3/3) and invasive carcinoma (4/4).

CONCLUSIONS

These results indicate that enhanced expression of telomerase activity occurs early during human oral carcinogenesis and support the critical role of telomerase in the development of human oral cancer.

Authors+Show Affiliations

Dental Research Institute, University of California, Los Angeles, 90095, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11595698

Citation

Kim, H R., et al. "Elevated Expression of hTERT Is Associated With Dysplastic Cell Transformation During Human Oral Carcinogenesis in Situ." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 7, no. 10, 2001, pp. 3079-86.
Kim HR, Christensen R, Park NH, et al. Elevated expression of hTERT is associated with dysplastic cell transformation during human oral carcinogenesis in situ. Clin Cancer Res. 2001;7(10):3079-86.
Kim, H. R., Christensen, R., Park, N. H., Sapp, P., Kang, M. K., & Park, N. H. (2001). Elevated expression of hTERT is associated with dysplastic cell transformation during human oral carcinogenesis in situ. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 7(10), 3079-86.
Kim HR, et al. Elevated Expression of hTERT Is Associated With Dysplastic Cell Transformation During Human Oral Carcinogenesis in Situ. Clin Cancer Res. 2001;7(10):3079-86. PubMed PMID: 11595698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated expression of hTERT is associated with dysplastic cell transformation during human oral carcinogenesis in situ. AU - Kim,H R, AU - Christensen,R, AU - Park,N H, AU - Sapp,P, AU - Kang,M K, AU - Park,N H, PY - 2001/10/12/pubmed PY - 2002/1/5/medline PY - 2001/10/12/entrez SP - 3079 EP - 86 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin Cancer Res VL - 7 IS - 10 N2 - PURPOSE: Telomerase is a ribonucleoprotein complex composed of the catalytic protein subunit (human telomerase reverse transcriptase or hTERT) and the RNA template. This enzyme activity is a necessary and rate-limiting step of cellular immortalization and could provide a unique marker of aberrant cells, which may selectively be targeted. The current study was undertaken to quantitatively determine the degree of telomerase activation during multistage oral carcinogenesis using paraffin-embedded tissue samples. EXPERIMENTAL DESIGN: hTERT expression level was quantitatively compared between normal and cancerous oral tissues by real-time reverse-transcription-PCR (RT-PCR). Also, the presence of hTERT transcript in individual cells was surveyed in the biopsy specimens with varying degrees of histopathology by in situ RT-PCR. RESULTS: Low level of hTERT amplification was detected by real-time RT-PCR in most (11/13) normal human oral mucosa. hTERT expression was also detected in the majority (11/12) of squamous cell carcinoma tissues, and the level was significantly (P < 0.05) elevated, on the average, by a factor >6.9. By in situ RT-PCR, hTERT expression was not noted in normal epithelium (0/10) nor in mild dysplasia (0/7) but was detected in moderate dysplasia (4/5) and in those tissues with a higher grade of histopathology: severe dysplasia (3/3) and invasive carcinoma (4/4). CONCLUSIONS: These results indicate that enhanced expression of telomerase activity occurs early during human oral carcinogenesis and support the critical role of telomerase in the development of human oral cancer. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/11595698/Elevated_expression_of_hTERT_is_associated_with_dysplastic_cell_transformation_during_human_oral_carcinogenesis_in_situ_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&amp;pmid=11595698 DB - PRIME DP - Unbound Medicine ER -