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Cathinone (Khat) and methcathinone (CAT) in urine specimens: a gas chromatographic-mass spectrometric detection procedure.
J Anal Toxicol. 2001 Oct; 25(7):525-30.JA

Abstract

A gas chromatographic-mass spectrometric procedure for detection of cathinone (Khat) and methcathinone (CAT) in urine was developed. The compounds were detected as 4-carboethoxyhexafluorobutyryl derivatives. Three ions for the drugs and two ions for the internal standards were monitored. The drugs were identified by comparing retention times and ion ratios with that of reference compounds. The concentrations were measured by using amphetamine-d6 as internal standard for cathinone and methamphetamine-d9 as internal standard for methcathinone, and were linear over the range of 25-5000 ng/mL for cathinone and 12.5-5000 ng/mL for methcathinone. The overall recoveries of cathinone and methcathinone were 86 and 78%, respectively. Intrarun and inter-run variations were < 20%. To verify that the drugs are not metabolites of over-the-counter medications, cathinone and methcathinone were tested in urine specimens collected from individuals who ingested phenylpropanolamine and pseudoephedrine. None of the specimens showed the keto-amines as the metabolic products. When the procedure was applied to test 66 amphetamine-immunoassay-positive specimens containing no amphetamine or methamphetamine, two specimens were found positive for cathinone (118 and 3266 ng/mL) and six specimens were found positive for methcathinone (13-91 ng/mL).

Authors+Show Affiliations

Division of Forensic Toxicology, Office of the Armed Forces Medical Examiner, Armed Forces Institute of Pathology, Rockville, Maryland 20850, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11599595

Citation

Paul, B D., and K A. Cole. "Cathinone (Khat) and Methcathinone (CAT) in Urine Specimens: a Gas Chromatographic-mass Spectrometric Detection Procedure." Journal of Analytical Toxicology, vol. 25, no. 7, 2001, pp. 525-30.
Paul BD, Cole KA. Cathinone (Khat) and methcathinone (CAT) in urine specimens: a gas chromatographic-mass spectrometric detection procedure. J Anal Toxicol. 2001;25(7):525-30.
Paul, B. D., & Cole, K. A. (2001). Cathinone (Khat) and methcathinone (CAT) in urine specimens: a gas chromatographic-mass spectrometric detection procedure. Journal of Analytical Toxicology, 25(7), 525-30.
Paul BD, Cole KA. Cathinone (Khat) and Methcathinone (CAT) in Urine Specimens: a Gas Chromatographic-mass Spectrometric Detection Procedure. J Anal Toxicol. 2001;25(7):525-30. PubMed PMID: 11599595.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cathinone (Khat) and methcathinone (CAT) in urine specimens: a gas chromatographic-mass spectrometric detection procedure. AU - Paul,B D, AU - Cole,K A, PY - 2001/10/16/pubmed PY - 2002/3/1/medline PY - 2001/10/16/entrez SP - 525 EP - 30 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 25 IS - 7 N2 - A gas chromatographic-mass spectrometric procedure for detection of cathinone (Khat) and methcathinone (CAT) in urine was developed. The compounds were detected as 4-carboethoxyhexafluorobutyryl derivatives. Three ions for the drugs and two ions for the internal standards were monitored. The drugs were identified by comparing retention times and ion ratios with that of reference compounds. The concentrations were measured by using amphetamine-d6 as internal standard for cathinone and methamphetamine-d9 as internal standard for methcathinone, and were linear over the range of 25-5000 ng/mL for cathinone and 12.5-5000 ng/mL for methcathinone. The overall recoveries of cathinone and methcathinone were 86 and 78%, respectively. Intrarun and inter-run variations were < 20%. To verify that the drugs are not metabolites of over-the-counter medications, cathinone and methcathinone were tested in urine specimens collected from individuals who ingested phenylpropanolamine and pseudoephedrine. None of the specimens showed the keto-amines as the metabolic products. When the procedure was applied to test 66 amphetamine-immunoassay-positive specimens containing no amphetamine or methamphetamine, two specimens were found positive for cathinone (118 and 3266 ng/mL) and six specimens were found positive for methcathinone (13-91 ng/mL). SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/11599595/Cathinone__Khat__and_methcathinone__CAT__in_urine_specimens:_a_gas_chromatographic_mass_spectrometric_detection_procedure_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/25.7.525 DB - PRIME DP - Unbound Medicine ER -