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[Transforming growth factor-beta 1 inhibits the generation of dendritic cells from murine hematopoietic progenitor cells in vitro].
Zhonghua Yi Xue Za Zhi. 1999 Mar; 79(3):178-80.ZY

Abstract

OBJECTIVE

To investigate the regulatory effect of transforming growth factor-beta 1 (TGF-beta 1) on the generation of dendritic cells (DCs) from hematopoietic progenitor cells (HPCs) and its cellular and molecular mechanisms.

METHODS

Murine bone marrow derived-Lin c-kit+ HPCs were isolated by a cell sorter and cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF), tumor necrosis factor alpha(TNF alpha) and TGF-beta 1. The generation of DC precursors and mature DCs and their phenotypes were analyzed by a cell sorter, reverse transcription-polymerase chain reaction (RT-PCR), and allogenic mixed lymphocyte reaction (MLR).

RESULTS

TGF-beta 1 could significantly inhibit the generation of DC precursors at day 6 and mature DCs at day 12 from murine Lin- c-kit+ HPCs stimulated with GM-CSF + SCF + TNF alpha or GM-CSF + SCF. Moreover, TGF-beta 1 markedly suppressed the expression of antigen peresenting molecule Ia and co-stimulatory molecule CD86 on the surface of cultured Lin- c-kit+ HPCs. Examination of RT-PCR showed that TGF-beta 1 could also inhibit the expression of MHC class II trans-activator (CTIIA) mRNA, which encodes a transcriptional factor to strictly control the expression of MHC class II antigens in the cultured cells.

CONCLUSION

Our results suggest that TGF-beta 1 is an inhibitory cytokine for the generation of DC precursors and maturation of DC from HPCs, which may account for the cellular mechanisms of TGF-beta 1-mediated immuno-suppressive functions in vivo and in vitro.

Authors+Show Affiliations

Immunology Research Unit, Suzhou Medical College, P.R. China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11601034

Citation

Zhang, Y, et al. "[Transforming Growth Factor-beta 1 Inhibits the Generation of Dendritic Cells From Murine Hematopoietic Progenitor Cells in Vitro]." Zhonghua Yi Xue Za Zhi, vol. 79, no. 3, 1999, pp. 178-80.
Zhang Y, Chen P, Zhang Y. [Transforming growth factor-beta 1 inhibits the generation of dendritic cells from murine hematopoietic progenitor cells in vitro]. Zhonghua Yi Xue Za Zhi. 1999;79(3):178-80.
Zhang, Y., Chen, P., & Zhang, Y. (1999). [Transforming growth factor-beta 1 inhibits the generation of dendritic cells from murine hematopoietic progenitor cells in vitro]. Zhonghua Yi Xue Za Zhi, 79(3), 178-80.
Zhang Y, Chen P, Zhang Y. [Transforming Growth Factor-beta 1 Inhibits the Generation of Dendritic Cells From Murine Hematopoietic Progenitor Cells in Vitro]. Zhonghua Yi Xue Za Zhi. 1999;79(3):178-80. PubMed PMID: 11601034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Transforming growth factor-beta 1 inhibits the generation of dendritic cells from murine hematopoietic progenitor cells in vitro]. AU - Zhang,Y, AU - Chen,P, AU - Zhang,Y, PY - 2001/10/17/pubmed PY - 2002/1/5/medline PY - 2001/10/17/entrez SP - 178 EP - 80 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 79 IS - 3 N2 - OBJECTIVE: To investigate the regulatory effect of transforming growth factor-beta 1 (TGF-beta 1) on the generation of dendritic cells (DCs) from hematopoietic progenitor cells (HPCs) and its cellular and molecular mechanisms. METHODS: Murine bone marrow derived-Lin c-kit+ HPCs were isolated by a cell sorter and cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF), tumor necrosis factor alpha(TNF alpha) and TGF-beta 1. The generation of DC precursors and mature DCs and their phenotypes were analyzed by a cell sorter, reverse transcription-polymerase chain reaction (RT-PCR), and allogenic mixed lymphocyte reaction (MLR). RESULTS: TGF-beta 1 could significantly inhibit the generation of DC precursors at day 6 and mature DCs at day 12 from murine Lin- c-kit+ HPCs stimulated with GM-CSF + SCF + TNF alpha or GM-CSF + SCF. Moreover, TGF-beta 1 markedly suppressed the expression of antigen peresenting molecule Ia and co-stimulatory molecule CD86 on the surface of cultured Lin- c-kit+ HPCs. Examination of RT-PCR showed that TGF-beta 1 could also inhibit the expression of MHC class II trans-activator (CTIIA) mRNA, which encodes a transcriptional factor to strictly control the expression of MHC class II antigens in the cultured cells. CONCLUSION: Our results suggest that TGF-beta 1 is an inhibitory cytokine for the generation of DC precursors and maturation of DC from HPCs, which may account for the cellular mechanisms of TGF-beta 1-mediated immuno-suppressive functions in vivo and in vitro. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/11601034/[Transforming_growth_factor_beta_1_inhibits_the_generation_of_dendritic_cells_from_murine_hematopoietic_progenitor_cells_in_vitro]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=1999&vol=79&issue=3&fpage=178 DB - PRIME DP - Unbound Medicine ER -