TY - JOUR T1 - The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing. AU - Davis,A P, AU - Symington,L S, PY - 2001/10/19/pubmed PY - 2002/1/16/medline PY - 2001/10/19/entrez SP - 515 EP - 25 JF - Genetics JO - Genetics VL - 159 IS - 2 N2 - The yeast RAD52 gene is essential for homology-dependent repair of DNA double-strand breaks. In vitro, Rad52 binds to single- and double-stranded DNA and promotes annealing of complementary single-stranded DNA. Genetic studies indicate that the Rad52 and Rad59 proteins act in the same recombination pathway either as a complex or through overlapping functions. Here we demonstrate physical interaction between Rad52 and Rad59 using the yeast two-hybrid system and co-immunoprecipitation from yeast extracts. Purified Rad59 efficiently anneals complementary oligonucleotides and is able to overcome the inhibition to annealing imposed by replication protein A (RPA). Although Rad59 has strand-annealing activity by itself in vitro, this activity is insufficient to promote strand annealing in vivo in the absence of Rad52. The rfa1-D288Y allele partially suppresses the in vivo strand-annealing defect of rad52 mutants, but this is independent of RAD59. These results suggest that in vivo Rad59 is unable to compete with RPA for single-stranded DNA and therefore is unable to promote single-strand annealing. Instead, Rad59 appears to augment the activity of Rad52 in strand annealing. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/11606529/The_yeast_recombinational_repair_protein_Rad59_interacts_with_Rad52_and_stimulates_single_strand_annealing_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1093/genetics/159.2.515 DB - PRIME DP - Unbound Medicine ER -