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[RAPD-marking of genomes of representative Hordeum species].
Genetika. 2001 Aug; 37(8):1088-94.G

Abstract

Random amplification of polymorphic DNA (RAPD) was used to analyze six species, three populations, and seven regional cultivars of barley. A unique pattern of amplified DNA products was obtained for each species of the genus Hordeum. High polymorphism of barley species was revealed. Specific fragments were found in most RAPD patterns; the fragments can be used as molecular markers of corresponding species and subspecies. Several other DNA fragments were shown to serve as molecular markers of the H genome. Specific RAPD patterns were obtained for each population and each cultivar of H. vulgare sensu lato. In total, variation between the populations and between the cultivars was substantially lower than between species. Cluster analysis (UPGMA) was used to estimate genetic distances between the Hordeum species, between the H. spontaneum populations, and between regional H. vulgare cultivars and a dendrogram was constructed.

Authors+Show Affiliations

Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia. kochieva@vigg.ruNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

11642109

Citation

Kochieva, E Z., et al. "[RAPD-marking of Genomes of Representative Hordeum Species]." Genetika, vol. 37, no. 8, 2001, pp. 1088-94.
Kochieva EZ, Goriunova SV, Pomortsev AA. [RAPD-marking of genomes of representative Hordeum species]. Genetika. 2001;37(8):1088-94.
Kochieva, E. Z., Goriunova, S. V., & Pomortsev, A. A. (2001). [RAPD-marking of genomes of representative Hordeum species]. Genetika, 37(8), 1088-94.
Kochieva EZ, Goriunova SV, Pomortsev AA. [RAPD-marking of Genomes of Representative Hordeum Species]. Genetika. 2001;37(8):1088-94. PubMed PMID: 11642109.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [RAPD-marking of genomes of representative Hordeum species]. AU - Kochieva,E Z, AU - Goriunova,S V, AU - Pomortsev,A A, PY - 2001/10/20/pubmed PY - 2002/1/17/medline PY - 2001/10/20/entrez SP - 1088 EP - 94 JF - Genetika JO - Genetika VL - 37 IS - 8 N2 - Random amplification of polymorphic DNA (RAPD) was used to analyze six species, three populations, and seven regional cultivars of barley. A unique pattern of amplified DNA products was obtained for each species of the genus Hordeum. High polymorphism of barley species was revealed. Specific fragments were found in most RAPD patterns; the fragments can be used as molecular markers of corresponding species and subspecies. Several other DNA fragments were shown to serve as molecular markers of the H genome. Specific RAPD patterns were obtained for each population and each cultivar of H. vulgare sensu lato. In total, variation between the populations and between the cultivars was substantially lower than between species. Cluster analysis (UPGMA) was used to estimate genetic distances between the Hordeum species, between the H. spontaneum populations, and between regional H. vulgare cultivars and a dendrogram was constructed. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/11642109/[RAPD_marking_of_genomes_of_representative_Hordeum_species]_ DB - PRIME DP - Unbound Medicine ER -