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Cytochemical localization of Na+/K+-ATPase activity in cochlear strial marginal cells after various catecholamine administrations.
Histol Histopathol. 2001 10; 16(4):1197-201.HH

Abstract

Sodium/potassium-activated adenosine triphosphatase (Na+/K+-ATPase) activity in the kidney and brain is high, and is regulated by catecholamines. Na+/K+-ATPase activity is also high in the basolateral infoldings of the strial marginal cells, where it aids in maintaining the characteristic electrolyte composition of the endolymph. To clarify the involvement of humoral control in strial function, particularly the role of catecholamines, the K+-dependent p-nitrophenylphosphatase (K+-NPPase) activity of strial marginal cells was investigated in guinea pigs using a cerium-based cytochemical method. The effects of reserpine, serotonin (5-HT), norepinephrine (NE), epinephrine (EP), both alone and in combination, were studied. High doses of reserpine cause depletion of sympathetic substances. Strial K+-NPPase activity was decreased after reserpine or dopamine treatment, and was increased after 5-HT, NE, and EP treatment. After reserpinization, repeated treatment with 5-HT, NE, or EP led to detectable strial enzyme activity. Thus, exogenous 5-HT, NE, and EP were able to restore strial K+-NPPase activity in the reserpine-treated animals. These results suggested that biogenic amines regulate strial K+-NPPase activity. Thus, the function of the stria vascularis may be regulated by the opposing actions of these catecholamines, and 5-HT.

Authors+Show Affiliations

Department of Otolaryngology, Hyogo College of Medicine, Nishiomiya, Japan. nkanoh@hyo-med.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

11642739

Citation

Kanoh, N, et al. "Cytochemical Localization of Na+/K+-ATPase Activity in Cochlear Strial Marginal Cells After Various Catecholamine Administrations." Histology and Histopathology, vol. 16, no. 4, 2001, pp. 1197-201.
Kanoh N, Dai CF, Mohri D, et al. Cytochemical localization of Na+/K+-ATPase activity in cochlear strial marginal cells after various catecholamine administrations. Histol Histopathol. 2001;16(4):1197-201.
Kanoh, N., Dai, C. F., Mohri, D., & Hori, S. (2001). Cytochemical localization of Na+/K+-ATPase activity in cochlear strial marginal cells after various catecholamine administrations. Histology and Histopathology, 16(4), 1197-201. https://doi.org/10.14670/HH-16.1197
Kanoh N, et al. Cytochemical Localization of Na+/K+-ATPase Activity in Cochlear Strial Marginal Cells After Various Catecholamine Administrations. Histol Histopathol. 2001;16(4):1197-201. PubMed PMID: 11642739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochemical localization of Na+/K+-ATPase activity in cochlear strial marginal cells after various catecholamine administrations. AU - Kanoh,N, AU - Dai,C F, AU - Mohri,D, AU - Hori,S, PY - 2001/10/20/pubmed PY - 2002/3/22/medline PY - 2001/10/20/entrez SP - 1197 EP - 201 JF - Histology and histopathology JO - Histol Histopathol VL - 16 IS - 4 N2 - Sodium/potassium-activated adenosine triphosphatase (Na+/K+-ATPase) activity in the kidney and brain is high, and is regulated by catecholamines. Na+/K+-ATPase activity is also high in the basolateral infoldings of the strial marginal cells, where it aids in maintaining the characteristic electrolyte composition of the endolymph. To clarify the involvement of humoral control in strial function, particularly the role of catecholamines, the K+-dependent p-nitrophenylphosphatase (K+-NPPase) activity of strial marginal cells was investigated in guinea pigs using a cerium-based cytochemical method. The effects of reserpine, serotonin (5-HT), norepinephrine (NE), epinephrine (EP), both alone and in combination, were studied. High doses of reserpine cause depletion of sympathetic substances. Strial K+-NPPase activity was decreased after reserpine or dopamine treatment, and was increased after 5-HT, NE, and EP treatment. After reserpinization, repeated treatment with 5-HT, NE, or EP led to detectable strial enzyme activity. Thus, exogenous 5-HT, NE, and EP were able to restore strial K+-NPPase activity in the reserpine-treated animals. These results suggested that biogenic amines regulate strial K+-NPPase activity. Thus, the function of the stria vascularis may be regulated by the opposing actions of these catecholamines, and 5-HT. SN - 0213-3911 UR - https://www.unboundmedicine.com/medline/citation/11642739/Cytochemical_localization_of_Na+/K+_ATPase_activity_in_cochlear_strial_marginal_cells_after_various_catecholamine_administrations_ DB - PRIME DP - Unbound Medicine ER -