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Effects of extending the duration of postgrafting immunosuppression and substituting granulocyte-colony-stimulating factor-mobilized peripheral blood mononuclear cells for marrow in allogeneic engraftment in a nonmyeloablative canine transplantation model.
Biol Blood Marrow Transplant. 2001; 7(9):513-6.BB

Abstract

Stable mixed donor/host hematopoietic chimerism was uniformly achieved in dogs given 200 cGy total body irradiation (TBI) before and immunosuppression with mycophenolate mofetil (MMF) for 28 days and cyclosporine (CSP) for 35 days after transplantation of marrow from dog leukocyte antigen-identical littermates. When the TBI dose was lowered to 100 cGy, donor marrow engraftment in 6 dogs was only transient, lasting 3 to 12 weeks. In this study, we asked whether stable engraftment in this model could be achieved: (1) by substituting recombinant canine granulocyte-colony-stimulating factor-mobilized peripheral blood mononuclear cells (G-PBMCs) for marrow and (2) by extending CSP administration from 35 to 100 days. Eighteen dogs were given G-PBMC grafts and MMF for 28 days. Eight of the 18 dogs received CSP for 35 days and 10 for 100 days. We found that substituting G-PBMCs for marrow did not increase the incidence of stable allogeneic engraftment (P = .11). However, increasing the duration of posttransplantation immunosuppression with CSP from 35 to 100 days favorably influenced stable donor engraftment (P = .06).

Authors+Show Affiliations

Clinical Research Division, Fred Hutchinson Cancer Research Center Washington, Seattle 98109-1024, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11669218

Citation

Zaucha, J M., et al. "Effects of Extending the Duration of Postgrafting Immunosuppression and Substituting Granulocyte-colony-stimulating Factor-mobilized Peripheral Blood Mononuclear Cells for Marrow in Allogeneic Engraftment in a Nonmyeloablative Canine Transplantation Model." Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation, vol. 7, no. 9, 2001, pp. 513-6.
Zaucha JM, Yu C, Zellmer E, et al. Effects of extending the duration of postgrafting immunosuppression and substituting granulocyte-colony-stimulating factor-mobilized peripheral blood mononuclear cells for marrow in allogeneic engraftment in a nonmyeloablative canine transplantation model. Biol Blood Marrow Transplant. 2001;7(9):513-6.
Zaucha, J. M., Yu, C., Zellmer, E., Takatu, A., Junghanss, C., Little, M. T., & Storb, R. (2001). Effects of extending the duration of postgrafting immunosuppression and substituting granulocyte-colony-stimulating factor-mobilized peripheral blood mononuclear cells for marrow in allogeneic engraftment in a nonmyeloablative canine transplantation model. Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation, 7(9), 513-6.
Zaucha JM, et al. Effects of Extending the Duration of Postgrafting Immunosuppression and Substituting Granulocyte-colony-stimulating Factor-mobilized Peripheral Blood Mononuclear Cells for Marrow in Allogeneic Engraftment in a Nonmyeloablative Canine Transplantation Model. Biol Blood Marrow Transplant. 2001;7(9):513-6. PubMed PMID: 11669218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of extending the duration of postgrafting immunosuppression and substituting granulocyte-colony-stimulating factor-mobilized peripheral blood mononuclear cells for marrow in allogeneic engraftment in a nonmyeloablative canine transplantation model. AU - Zaucha,J M, AU - Yu,C, AU - Zellmer,E, AU - Takatu,A, AU - Junghanss,C, AU - Little,M T, AU - Storb,R, PY - 2001/10/24/pubmed PY - 2002/3/30/medline PY - 2001/10/24/entrez SP - 513 EP - 6 JF - Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation JO - Biol. Blood Marrow Transplant. VL - 7 IS - 9 N2 - Stable mixed donor/host hematopoietic chimerism was uniformly achieved in dogs given 200 cGy total body irradiation (TBI) before and immunosuppression with mycophenolate mofetil (MMF) for 28 days and cyclosporine (CSP) for 35 days after transplantation of marrow from dog leukocyte antigen-identical littermates. When the TBI dose was lowered to 100 cGy, donor marrow engraftment in 6 dogs was only transient, lasting 3 to 12 weeks. In this study, we asked whether stable engraftment in this model could be achieved: (1) by substituting recombinant canine granulocyte-colony-stimulating factor-mobilized peripheral blood mononuclear cells (G-PBMCs) for marrow and (2) by extending CSP administration from 35 to 100 days. Eighteen dogs were given G-PBMC grafts and MMF for 28 days. Eight of the 18 dogs received CSP for 35 days and 10 for 100 days. We found that substituting G-PBMCs for marrow did not increase the incidence of stable allogeneic engraftment (P = .11). However, increasing the duration of posttransplantation immunosuppression with CSP from 35 to 100 days favorably influenced stable donor engraftment (P = .06). SN - 1083-8791 UR - https://www.unboundmedicine.com/medline/citation/11669218/Effects_of_extending_the_duration_of_postgrafting_immunosuppression_and_substituting_granulocyte_colony_stimulating_factor_mobilized_peripheral_blood_mononuclear_cells_for_marrow_in_allogeneic_engraftment_in_a_nonmyeloablative_canine_transplantation_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1083-8791(01)50039-7 DB - PRIME DP - Unbound Medicine ER -