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Estrogen inhibits lipopolysaccharide-induced tumor necrosis factor-alpha release from murine macrophages.
Methods Find Exp Clin Pharmacol. 2001 May; 23(4):169-73.MF

Abstract

During their reproductive years, female have a lower risk for atherosclerosis as compared with age-matched males, although the mechanisms behind this are not clearly understood. Cytokines, including TNF-alpha play an important role in the pathogenesis of atherosclerosis. We therefore evaluated whether or not there was any difference between 17 beta-estradiol and testosterone in modulating TNF-alpha release from murine bone marrow-derived macrophages (BMM) in vitro. Cells were incubated with or without physiological concentrations (10(-10)-10(-8) M) of 17 beta-estradiol or testosterone for 48 h, followed by an additional 6 h in the absence or presence of lipopolysaccharide (LPS; 10 micrograms/ml). The amount of TNF-alpha released into the culture medium was determined with radioimmunoassay. We found that 17 beta-estradiol or testosterone alone did not affect TNF-alpha release from BMM as compared to untreated controls. Preincubation with 17 beta-estradiol significantly inhibited LPS-induced TNF-alpha release by 18.15% (p < 0.05). 25.28% (p < 0.05) and 40.83% (p < 0.01) for 10(-10), 10(-9) and 10(-8) M of 17 beta-estradiol, respectively, as compared to LPS alone. In contrast, testosterone tested for 3 concentrations did not significantly effect TNF-alpha release induced by LPS. The results indicate that 17 beta-estradiol, but not testosterone, inhibits TNF-alpha release from LPS-stimulated macrophages, which may be one of the mechanisms by which estrogen protects against atherosclerosis.

Authors+Show Affiliations

Laboratory of Molecular Biology, Shanxi Medical University, Taiyuan, P.R. China. zdqiao@public.ty.sx.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11676224

Citation

Zhang, X, et al. "Estrogen Inhibits Lipopolysaccharide-induced Tumor Necrosis Factor-alpha Release From Murine Macrophages." Methods and Findings in Experimental and Clinical Pharmacology, vol. 23, no. 4, 2001, pp. 169-73.
Zhang X, Wang L, Zhang H, et al. Estrogen inhibits lipopolysaccharide-induced tumor necrosis factor-alpha release from murine macrophages. Methods Find Exp Clin Pharmacol. 2001;23(4):169-73.
Zhang, X., Wang, L., Zhang, H., Guo, D., Qiao, Z., & Qiao, J. (2001). Estrogen inhibits lipopolysaccharide-induced tumor necrosis factor-alpha release from murine macrophages. Methods and Findings in Experimental and Clinical Pharmacology, 23(4), 169-73.
Zhang X, et al. Estrogen Inhibits Lipopolysaccharide-induced Tumor Necrosis Factor-alpha Release From Murine Macrophages. Methods Find Exp Clin Pharmacol. 2001;23(4):169-73. PubMed PMID: 11676224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estrogen inhibits lipopolysaccharide-induced tumor necrosis factor-alpha release from murine macrophages. AU - Zhang,X, AU - Wang,L, AU - Zhang,H, AU - Guo,D, AU - Qiao,Z, AU - Qiao,J, PY - 2001/10/26/pubmed PY - 2002/4/4/medline PY - 2001/10/26/entrez SP - 169 EP - 73 JF - Methods and findings in experimental and clinical pharmacology JO - Methods Find Exp Clin Pharmacol VL - 23 IS - 4 N2 - During their reproductive years, female have a lower risk for atherosclerosis as compared with age-matched males, although the mechanisms behind this are not clearly understood. Cytokines, including TNF-alpha play an important role in the pathogenesis of atherosclerosis. We therefore evaluated whether or not there was any difference between 17 beta-estradiol and testosterone in modulating TNF-alpha release from murine bone marrow-derived macrophages (BMM) in vitro. Cells were incubated with or without physiological concentrations (10(-10)-10(-8) M) of 17 beta-estradiol or testosterone for 48 h, followed by an additional 6 h in the absence or presence of lipopolysaccharide (LPS; 10 micrograms/ml). The amount of TNF-alpha released into the culture medium was determined with radioimmunoassay. We found that 17 beta-estradiol or testosterone alone did not affect TNF-alpha release from BMM as compared to untreated controls. Preincubation with 17 beta-estradiol significantly inhibited LPS-induced TNF-alpha release by 18.15% (p < 0.05). 25.28% (p < 0.05) and 40.83% (p < 0.01) for 10(-10), 10(-9) and 10(-8) M of 17 beta-estradiol, respectively, as compared to LPS alone. In contrast, testosterone tested for 3 concentrations did not significantly effect TNF-alpha release induced by LPS. The results indicate that 17 beta-estradiol, but not testosterone, inhibits TNF-alpha release from LPS-stimulated macrophages, which may be one of the mechanisms by which estrogen protects against atherosclerosis. SN - 0379-0355 UR - https://www.unboundmedicine.com/medline/citation/11676224/full_citation L2 - http://journals.prous.com/journals/servlet/xmlxsl/pk_journals.xml_summaryn_pr?p_JournalId=6&amp;p_RefId=634640 DB - PRIME DP - Unbound Medicine ER -