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Transcriptional regulation of the antioxidant protein 2 gene, a thiol-specific antioxidant, by lens epithelium-derived growth factor to protect cells from oxidative stress.
J Biol Chem. 2001 Dec 28; 276(52):48899-907.JB

Abstract

Antioxidant protein 2 (AOP2), a member of the newly defined family of thiol-specific antioxidant proteins, has been shown to remove H(2)O(2) and protect proteins and DNA from oxidative stress. Here we report that LEDGF is one of the regulatory factors for the AOP2 gene. We found that LEDGF bound to the heat shock element and to stress-related elements in the AOP2 promoter. It trans-activated expression of AOP2-CAT in COS-7 cells and lens epithelial cells overexpressing LEDGF. Mutations in the heat shock element and stress-related elements of the AOP2 promoter reduced LEDGF-dependent trans-activation. Lens epithelial cells showed a higher level of AOP2 mRNA in the presence of LEDGF. Cells overexpressing LEDGF exhibited a higher level of AOP2 protein, the level of which was directly related to the increase in cellular protection. Thus, LEDGF, by activating the AOP2 gene, protected and enhanced the survival of cells under oxidative stress.

Authors+Show Affiliations

Center for Ophthalmic Research, Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11677226

Citation

Fatma, N, et al. "Transcriptional Regulation of the Antioxidant Protein 2 Gene, a Thiol-specific Antioxidant, By Lens Epithelium-derived Growth Factor to Protect Cells From Oxidative Stress." The Journal of Biological Chemistry, vol. 276, no. 52, 2001, pp. 48899-907.
Fatma N, Singh DP, Shinohara T, et al. Transcriptional regulation of the antioxidant protein 2 gene, a thiol-specific antioxidant, by lens epithelium-derived growth factor to protect cells from oxidative stress. J Biol Chem. 2001;276(52):48899-907.
Fatma, N., Singh, D. P., Shinohara, T., & Chylack, L. T. (2001). Transcriptional regulation of the antioxidant protein 2 gene, a thiol-specific antioxidant, by lens epithelium-derived growth factor to protect cells from oxidative stress. The Journal of Biological Chemistry, 276(52), 48899-907.
Fatma N, et al. Transcriptional Regulation of the Antioxidant Protein 2 Gene, a Thiol-specific Antioxidant, By Lens Epithelium-derived Growth Factor to Protect Cells From Oxidative Stress. J Biol Chem. 2001 Dec 28;276(52):48899-907. PubMed PMID: 11677226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional regulation of the antioxidant protein 2 gene, a thiol-specific antioxidant, by lens epithelium-derived growth factor to protect cells from oxidative stress. AU - Fatma,N, AU - Singh,D P, AU - Shinohara,T, AU - Chylack,L T,Jr Y1 - 2001/10/24/ PY - 2001/10/26/pubmed PY - 2002/2/1/medline PY - 2001/10/26/entrez SP - 48899 EP - 907 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 276 IS - 52 N2 - Antioxidant protein 2 (AOP2), a member of the newly defined family of thiol-specific antioxidant proteins, has been shown to remove H(2)O(2) and protect proteins and DNA from oxidative stress. Here we report that LEDGF is one of the regulatory factors for the AOP2 gene. We found that LEDGF bound to the heat shock element and to stress-related elements in the AOP2 promoter. It trans-activated expression of AOP2-CAT in COS-7 cells and lens epithelial cells overexpressing LEDGF. Mutations in the heat shock element and stress-related elements of the AOP2 promoter reduced LEDGF-dependent trans-activation. Lens epithelial cells showed a higher level of AOP2 mRNA in the presence of LEDGF. Cells overexpressing LEDGF exhibited a higher level of AOP2 protein, the level of which was directly related to the increase in cellular protection. Thus, LEDGF, by activating the AOP2 gene, protected and enhanced the survival of cells under oxidative stress. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/11677226/Transcriptional_regulation_of_the_antioxidant_protein_2_gene_a_thiol_specific_antioxidant_by_lens_epithelium_derived_growth_factor_to_protect_cells_from_oxidative_stress_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=11677226 DB - PRIME DP - Unbound Medicine ER -