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Clinical significance of nested polymerase chain reaction and immunofluorescence for detection of Pneumocystis carinii pneumonia.
Clin Microbiol Infect. 2001 Sep; 7(9):492-7.CM

Abstract

OBJECTIVE

To study the clinical significance of a nested polymerase chain reaction (PCR) method compared to immunofluorescence (IF) for detection of Pneumocystis carinii.

METHODS

The medical records of 89 patients with 91 episodes of pneumonia were scrutinised retrospectively. The pneumonia episodes were divided into categories according to the likelihood that the patient had had clinical Pneumocystis carinii pneumonia (PCP). All respiratory tract samples from the 89 patients (34 broncho-alveolar lavage (BAL) and 57 sputa) were tested for Pneumocystis carinii by IF and nested PCR.

RESULTS

Fifteen episodes, as diagnosed by IF, were classified as true PCP (combination of the groups with definite and probable PCP; sensitivity 60%, specificity 97%). Among the P. carinii DNA-positive episodes, detected with nested PCR, 24 were classified as true PCP (combination of the groups with definite and probable PCP; sensitivity 96%, specificity 59%), since all IF-positive samples were nested PCR positive. Only one pneumonia episode classified as a probable PCP, was negative with both methods, as applied to a BAL sample.

CONCLUSIONS

IF applied to BAL or sputum seems to be the most specific method for diagnosis of clinical PCP. Additional clinical cases can be found by nested PCR, although this then gives a high risk of detecting subclinical colonisation of P. carinii.

Authors+Show Affiliations

Department of Parasitology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden. mats.wahlgren@smi.ki.seNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

11678932

Citation

Olsson, M, et al. "Clinical Significance of Nested Polymerase Chain Reaction and Immunofluorescence for Detection of Pneumocystis Carinii Pneumonia." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 7, no. 9, 2001, pp. 492-7.
Olsson M, Strålin K, Holmberg H. Clinical significance of nested polymerase chain reaction and immunofluorescence for detection of Pneumocystis carinii pneumonia. Clin Microbiol Infect. 2001;7(9):492-7.
Olsson, M., Strålin, K., & Holmberg, H. (2001). Clinical significance of nested polymerase chain reaction and immunofluorescence for detection of Pneumocystis carinii pneumonia. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 7(9), 492-7.
Olsson M, Strålin K, Holmberg H. Clinical Significance of Nested Polymerase Chain Reaction and Immunofluorescence for Detection of Pneumocystis Carinii Pneumonia. Clin Microbiol Infect. 2001;7(9):492-7. PubMed PMID: 11678932.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical significance of nested polymerase chain reaction and immunofluorescence for detection of Pneumocystis carinii pneumonia. AU - Olsson,M, AU - Strålin,K, AU - Holmberg,H, PY - 2001/10/27/pubmed PY - 2002/3/19/medline PY - 2001/10/27/entrez SP - 492 EP - 7 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin Microbiol Infect VL - 7 IS - 9 N2 - OBJECTIVE: To study the clinical significance of a nested polymerase chain reaction (PCR) method compared to immunofluorescence (IF) for detection of Pneumocystis carinii. METHODS: The medical records of 89 patients with 91 episodes of pneumonia were scrutinised retrospectively. The pneumonia episodes were divided into categories according to the likelihood that the patient had had clinical Pneumocystis carinii pneumonia (PCP). All respiratory tract samples from the 89 patients (34 broncho-alveolar lavage (BAL) and 57 sputa) were tested for Pneumocystis carinii by IF and nested PCR. RESULTS: Fifteen episodes, as diagnosed by IF, were classified as true PCP (combination of the groups with definite and probable PCP; sensitivity 60%, specificity 97%). Among the P. carinii DNA-positive episodes, detected with nested PCR, 24 were classified as true PCP (combination of the groups with definite and probable PCP; sensitivity 96%, specificity 59%), since all IF-positive samples were nested PCR positive. Only one pneumonia episode classified as a probable PCP, was negative with both methods, as applied to a BAL sample. CONCLUSIONS: IF applied to BAL or sputum seems to be the most specific method for diagnosis of clinical PCP. Additional clinical cases can be found by nested PCR, although this then gives a high risk of detecting subclinical colonisation of P. carinii. SN - 1198-743X UR - https://www.unboundmedicine.com/medline/citation/11678932/Clinical_significance_of_nested_polymerase_chain_reaction_and_immunofluorescence_for_detection_of_Pneumocystis_carinii_pneumonia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)64038-6 DB - PRIME DP - Unbound Medicine ER -