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Synthesis of polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae by using intermediates of fatty acid beta-oxidation.
Appl Environ Microbiol. 2001 Nov; 67(11):5254-60.AE

Abstract

Medium-chain-length polyhydroxyalkanoates (PHAs) are polyesters having properties of biodegradable thermoplastics and elastomers that are naturally produced by a variety of pseudomonads. Saccharomyces cerevisiae was transformed with the Pseudomonas aeruginosa PHAC1 synthase modified for peroxisome targeting by the addition of the carboxyl 34 amino acids from the Brassica napus isocitrate lyase. The PHAC1 gene was put under the control of the promoter of the catalase A gene. PHA synthase expression and PHA accumulation were found in recombinant S. cerevisiae growing in media containing fatty acids. PHA containing even-chain monomers from 6 to 14 carbons was found in recombinant yeast grown on oleic acid, while odd-chain monomers from 5 to 15 carbons were found in PHA from yeast grown on heptadecenoic acid. The maximum amount of PHA accumulated was 0.45% of the dry weight. Transmission electron microscopy of recombinant yeast grown on oleic acid revealed the presence of numerous PHA inclusions found within membrane-bound organelles. Together, these data show that S. cerevisiae expressing a peroxisomal PHA synthase produces PHA in the peroxisome using the 3-hydroxyacyl coenzyme A intermediates of the beta-oxidation of fatty acids present in the media. S. cerevisiae can thus be used as a powerful model system to learn how fatty acid metabolism can be modified in order to synthesize high amounts of PHA in eukaryotes, including plants.

Authors+Show Affiliations

Laboratoire de Biotechnologie Végétale, Institut d'Ecologie, Université de Lausanne, CH-1015 Lausanne, Switzerland. yves.poirier@ie-bpv.unil.chNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11679353

Citation

Poirier, Y, et al. "Synthesis of Polyhydroxyalkanoate in the Peroxisome of Saccharomyces Cerevisiae By Using Intermediates of Fatty Acid Beta-oxidation." Applied and Environmental Microbiology, vol. 67, no. 11, 2001, pp. 5254-60.
Poirier Y, Erard N, Petétot JM. Synthesis of polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae by using intermediates of fatty acid beta-oxidation. Appl Environ Microbiol. 2001;67(11):5254-60.
Poirier, Y., Erard, N., & Petétot, J. M. (2001). Synthesis of polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae by using intermediates of fatty acid beta-oxidation. Applied and Environmental Microbiology, 67(11), 5254-60.
Poirier Y, Erard N, Petétot JM. Synthesis of Polyhydroxyalkanoate in the Peroxisome of Saccharomyces Cerevisiae By Using Intermediates of Fatty Acid Beta-oxidation. Appl Environ Microbiol. 2001;67(11):5254-60. PubMed PMID: 11679353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis of polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae by using intermediates of fatty acid beta-oxidation. AU - Poirier,Y, AU - Erard,N, AU - Petétot,J M, PY - 2001/10/27/pubmed PY - 2002/1/18/medline PY - 2001/10/27/entrez SP - 5254 EP - 60 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 67 IS - 11 N2 - Medium-chain-length polyhydroxyalkanoates (PHAs) are polyesters having properties of biodegradable thermoplastics and elastomers that are naturally produced by a variety of pseudomonads. Saccharomyces cerevisiae was transformed with the Pseudomonas aeruginosa PHAC1 synthase modified for peroxisome targeting by the addition of the carboxyl 34 amino acids from the Brassica napus isocitrate lyase. The PHAC1 gene was put under the control of the promoter of the catalase A gene. PHA synthase expression and PHA accumulation were found in recombinant S. cerevisiae growing in media containing fatty acids. PHA containing even-chain monomers from 6 to 14 carbons was found in recombinant yeast grown on oleic acid, while odd-chain monomers from 5 to 15 carbons were found in PHA from yeast grown on heptadecenoic acid. The maximum amount of PHA accumulated was 0.45% of the dry weight. Transmission electron microscopy of recombinant yeast grown on oleic acid revealed the presence of numerous PHA inclusions found within membrane-bound organelles. Together, these data show that S. cerevisiae expressing a peroxisomal PHA synthase produces PHA in the peroxisome using the 3-hydroxyacyl coenzyme A intermediates of the beta-oxidation of fatty acids present in the media. S. cerevisiae can thus be used as a powerful model system to learn how fatty acid metabolism can be modified in order to synthesize high amounts of PHA in eukaryotes, including plants. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/11679353/Synthesis_of_polyhydroxyalkanoate_in_the_peroxisome_of_Saccharomyces_cerevisiae_by_using_intermediates_of_fatty_acid_beta_oxidation_ L2 - https://journals.asm.org/doi/10.1128/AEM.67.11.5254-5260.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -