Tags

Type your tag names separated by a space and hit enter

Analysis of flavonoids in Semen Cuscutae by micellar electrokinetic chromatography and high-performance liquid chromatography.
J Capill Electrophor Microchip Technol. 1999 Sep-Dec; 6(5-6):157-62.JC

Abstract

Five different flavonoids in Semen Cuscutae were determined for the first time by micellar electrokinetic chromatography (MEKC) and reversed-phase high-performance liquid chromatography (RP-HPLC). MEKC was carried out on a 68.5-cm fused-silica capillary using 0.035 mol/L sodium dodecyl sulfate and 0.02 mol/L borate-phosphate (pH 7.0) containing 10% methanol as the running buffer. RP-HPLC was performed on a 12.5-cm Hypersil C18 (Agilent Technologies, Palo Alto, CA, U.S.A.) column with a 0.025-mol/L phosphate acid (A) - methanol (B) solvent gradient. Both MEKC and HPLC are rapid, simple, and efficient. The results obtained using these techniques are compared. MEKC and HPLC can be complementary to each other for the quality control of Semen Cuscutae.

Authors+Show Affiliations

Li Y
Department of Chemistry, Peking University, Beijing, PR China.
Ye M
No affiliation info available
Liu H
No affiliation info available
Ji X
No affiliation info available
Yan Y
No affiliation info available

MeSH

CalibrationChromatography, High Pressure LiquidChromatography, Micellar Electrokinetic CapillaryDisaccharidesDrugs, Chinese HerbalFlavonoidsKaempferolsMicellesMolecular StructurePlants, MedicinalQuercetinSeedsSolvents

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11681521

Citation

Li, Y, et al. "Analysis of Flavonoids in Semen Cuscutae By Micellar Electrokinetic Chromatography and High-performance Liquid Chromatography." Journal of Capillary Electrophoresis and Microchip Technology, vol. 6, no. 5-6, 1999, pp. 157-62.
Li Y, Ye M, Liu H, et al. Analysis of flavonoids in Semen Cuscutae by micellar electrokinetic chromatography and high-performance liquid chromatography. J Capill Electrophor Microchip Technol. 1999;6(5-6):157-62.
Li, Y., Ye, M., Liu, H., Ji, X., & Yan, Y. (1999). Analysis of flavonoids in Semen Cuscutae by micellar electrokinetic chromatography and high-performance liquid chromatography. Journal of Capillary Electrophoresis and Microchip Technology, 6(5-6), 157-62.
Li Y, et al. Analysis of Flavonoids in Semen Cuscutae By Micellar Electrokinetic Chromatography and High-performance Liquid Chromatography. J Capill Electrophor Microchip Technol. 1999 Sep-Dec;6(5-6):157-62. PubMed PMID: 11681521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of flavonoids in Semen Cuscutae by micellar electrokinetic chromatography and high-performance liquid chromatography. AU - Li,Y, AU - Ye,M, AU - Liu,H, AU - Ji,X, AU - Yan,Y, PY - 2001/10/30/pubmed PY - 2002/1/5/medline PY - 2001/10/30/entrez SP - 157 EP - 62 JF - Journal of capillary electrophoresis and microchip technology JO - J Capill Electrophor Microchip Technol VL - 6 IS - 5-6 N2 - Five different flavonoids in Semen Cuscutae were determined for the first time by micellar electrokinetic chromatography (MEKC) and reversed-phase high-performance liquid chromatography (RP-HPLC). MEKC was carried out on a 68.5-cm fused-silica capillary using 0.035 mol/L sodium dodecyl sulfate and 0.02 mol/L borate-phosphate (pH 7.0) containing 10% methanol as the running buffer. RP-HPLC was performed on a 12.5-cm Hypersil C18 (Agilent Technologies, Palo Alto, CA, U.S.A.) column with a 0.025-mol/L phosphate acid (A) - methanol (B) solvent gradient. Both MEKC and HPLC are rapid, simple, and efficient. The results obtained using these techniques are compared. MEKC and HPLC can be complementary to each other for the quality control of Semen Cuscutae. SN - 1946-4940 UR - https://www.unboundmedicine.com/medline/citation/11681521/Analysis_of_flavonoids_in_Semen_Cuscutae_by_micellar_electrokinetic_chromatography_and_high_performance_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -