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GFP-like chromoproteins as a source of far-red fluorescent proteins.
FEBS Lett 2001; 507(1):16-20FL

Abstract

We have employed a new approach to generate novel fluorescent proteins (FPs) from red absorbing chromoproteins. An identical single amino acid substitution converted novel chromoproteins from the species Anthozoa (Heteractis crispa, Condylactis gigantea, and Goniopora tenuidens) into far-red FPs (emission lambda(max)=615-640 nm). Moreover, coupled site-directed and random mutagenesis of the chromoprotein from H. crispa resulted in a unique far-red FP (HcRed) that exhibited bright emission at 645 nm. A clear red shift in fluorescence of HcRed, compared to drFP583 (by more than 60 nm), makes it an ideal additional color for multi-color labeling. Importantly, HcRed is excitable by 600 nm dye laser, thus promoting new detection channels for multi-color flow cytometry applications. In addition, we generated a dimeric mutant with similar maturation and spectral properties to tetrameric HcRed.

Authors+Show Affiliations

Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11682051

Citation

Gurskaya, N G., et al. "GFP-like Chromoproteins as a Source of Far-red Fluorescent Proteins." FEBS Letters, vol. 507, no. 1, 2001, pp. 16-20.
Gurskaya NG, Fradkov AF, Terskikh A, et al. GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett. 2001;507(1):16-20.
Gurskaya, N. G., Fradkov, A. F., Terskikh, A., Matz, M. V., Labas, Y. A., Martynov, V. I., ... Lukyanov, S. A. (2001). GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Letters, 507(1), pp. 16-20.
Gurskaya NG, et al. GFP-like Chromoproteins as a Source of Far-red Fluorescent Proteins. FEBS Lett. 2001 Oct 19;507(1):16-20. PubMed PMID: 11682051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GFP-like chromoproteins as a source of far-red fluorescent proteins. AU - Gurskaya,N G, AU - Fradkov,A F, AU - Terskikh,A, AU - Matz,M V, AU - Labas,Y A, AU - Martynov,V I, AU - Yanushevich,Y G, AU - Lukyanov,K A, AU - Lukyanov,S A, PY - 2001/10/30/pubmed PY - 2002/1/5/medline PY - 2001/10/30/entrez SP - 16 EP - 20 JF - FEBS letters JO - FEBS Lett. VL - 507 IS - 1 N2 - We have employed a new approach to generate novel fluorescent proteins (FPs) from red absorbing chromoproteins. An identical single amino acid substitution converted novel chromoproteins from the species Anthozoa (Heteractis crispa, Condylactis gigantea, and Goniopora tenuidens) into far-red FPs (emission lambda(max)=615-640 nm). Moreover, coupled site-directed and random mutagenesis of the chromoprotein from H. crispa resulted in a unique far-red FP (HcRed) that exhibited bright emission at 645 nm. A clear red shift in fluorescence of HcRed, compared to drFP583 (by more than 60 nm), makes it an ideal additional color for multi-color labeling. Importantly, HcRed is excitable by 600 nm dye laser, thus promoting new detection channels for multi-color flow cytometry applications. In addition, we generated a dimeric mutant with similar maturation and spectral properties to tetrameric HcRed. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/11682051/GFP_like_chromoproteins_as_a_source_of_far_red_fluorescent_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(01)02930-1 DB - PRIME DP - Unbound Medicine ER -