Tags

Type your tag names separated by a space and hit enter

Astroglial and microglial reaction after a partial nigrostriatal degeneration induced by the striatal injection of different doses of 6-hydroxydopamine.

Abstract

Astroglial and microglial activation was analyzed in adult male Wistar rats after a unilateral striatal injection of different doses (8, 4 and 1 micrograms) of 6-hydroxydopamine (6-OHDA). Control animals received the injection of the same volume of the solvent. The rotational behavior was registered by a rotometer 24 and 72 hours, 7, 10, 14 and 22 days after lesion. Following, animals were sacrificed and the tyrosine hydroxylase (TH) positive dopamine cells, the glial fibrillary acidic protein (GFAP) immunolabeled astrocytes and the OX42 immunoreactive microglia were visualized by mean of immunohistochemistry and quantified by stereologic method employing the optical disector and the point intercepts. The apomorphine (0.5 mg/kg)-induced circling behavior was seen only after 8 micrograms of 6-OHDA from 72 hours postlesion until sacrifice. Decreases of the TH immunoreactive terminals and cell bodies were found in the sampled fields of the striatum and pars compacta of the substantia nigra (SNc), respectively, after 8 and 4 micrograms of 6-OHDA. The GFAP immunohistochemistry revealed increases in the number/density of astroglial cells in the ipsilateral neostriatum (137% of control) and ipsilateral SNc (83% of control) and also in the volumeal fraction of the astroglial processes in the ipsilateral neostriatum (30% of control) and ipsilateral SNc (38% of control) in the rats with higher dose of the neurotoxin. Increases in the number of OX42 microglial labeled profiles and in the volumeal fraction of microglial processes were found in the ipsilateral neostriatum (67% and 27%, respectively, of control) and ipsilateral SNc (100% and 50%, respectively, of control) in the 8 micrograms 6-OHDA injected rats. These results suggest that the retrograde degeneration induced by a intrastriatal injection of a small dose of the 6-OHDA leads to an astroglial and microglial reaction in the nigrostriatal dopamine pathway. The interaction between activated glial cells may be involved in the wounding and repair events in the partial lesioned nigrostriatal system as well as in the paracrine responses to surviving dopamine neurons.

Authors+Show Affiliations

,

Laboratory of Neuroregeneration, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 2415 05508-900-São Paulo, Brazil.

,

Source

MeSH

Animals
Astrocytes
Behavior, Animal
Corpus Striatum
Dose-Response Relationship, Drug
Glial Fibrillary Acidic Protein
Immunohistochemistry
Male
Microglia
Nerve Degeneration
Oxidopamine
Rats
Rats, Wistar
Substantia Nigra
Sympatholytics
Tyrosine 3-Monooxygenase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11699344

Citation

Rodrigues, R W., et al. "Astroglial and Microglial Reaction After a Partial Nigrostriatal Degeneration Induced By the Striatal Injection of Different Doses of 6-hydroxydopamine." The International Journal of Neuroscience, vol. 109, no. 1-2, 2001, pp. 91-126.
Rodrigues RW, Gomide VC, Chadi G. Astroglial and microglial reaction after a partial nigrostriatal degeneration induced by the striatal injection of different doses of 6-hydroxydopamine. Int J Neurosci. 2001;109(1-2):91-126.
Rodrigues, R. W., Gomide, V. C., & Chadi, G. (2001). Astroglial and microglial reaction after a partial nigrostriatal degeneration induced by the striatal injection of different doses of 6-hydroxydopamine. The International Journal of Neuroscience, 109(1-2), pp. 91-126.
Rodrigues RW, Gomide VC, Chadi G. Astroglial and Microglial Reaction After a Partial Nigrostriatal Degeneration Induced By the Striatal Injection of Different Doses of 6-hydroxydopamine. Int J Neurosci. 2001;109(1-2):91-126. PubMed PMID: 11699344.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Astroglial and microglial reaction after a partial nigrostriatal degeneration induced by the striatal injection of different doses of 6-hydroxydopamine. AU - Rodrigues,R W, AU - Gomide,V C, AU - Chadi,G, PY - 2001/11/9/pubmed PY - 2002/2/22/medline PY - 2001/11/9/entrez SP - 91 EP - 126 JF - The International journal of neuroscience JO - Int. J. Neurosci. VL - 109 IS - 1-2 N2 - Astroglial and microglial activation was analyzed in adult male Wistar rats after a unilateral striatal injection of different doses (8, 4 and 1 micrograms) of 6-hydroxydopamine (6-OHDA). Control animals received the injection of the same volume of the solvent. The rotational behavior was registered by a rotometer 24 and 72 hours, 7, 10, 14 and 22 days after lesion. Following, animals were sacrificed and the tyrosine hydroxylase (TH) positive dopamine cells, the glial fibrillary acidic protein (GFAP) immunolabeled astrocytes and the OX42 immunoreactive microglia were visualized by mean of immunohistochemistry and quantified by stereologic method employing the optical disector and the point intercepts. The apomorphine (0.5 mg/kg)-induced circling behavior was seen only after 8 micrograms of 6-OHDA from 72 hours postlesion until sacrifice. Decreases of the TH immunoreactive terminals and cell bodies were found in the sampled fields of the striatum and pars compacta of the substantia nigra (SNc), respectively, after 8 and 4 micrograms of 6-OHDA. The GFAP immunohistochemistry revealed increases in the number/density of astroglial cells in the ipsilateral neostriatum (137% of control) and ipsilateral SNc (83% of control) and also in the volumeal fraction of the astroglial processes in the ipsilateral neostriatum (30% of control) and ipsilateral SNc (38% of control) in the rats with higher dose of the neurotoxin. Increases in the number of OX42 microglial labeled profiles and in the volumeal fraction of microglial processes were found in the ipsilateral neostriatum (67% and 27%, respectively, of control) and ipsilateral SNc (100% and 50%, respectively, of control) in the 8 micrograms 6-OHDA injected rats. These results suggest that the retrograde degeneration induced by a intrastriatal injection of a small dose of the 6-OHDA leads to an astroglial and microglial reaction in the nigrostriatal dopamine pathway. The interaction between activated glial cells may be involved in the wounding and repair events in the partial lesioned nigrostriatal system as well as in the paracrine responses to surviving dopamine neurons. SN - 0020-7454 UR - https://www.unboundmedicine.com/medline/citation/11699344/Astroglial_and_microglial_reaction_after_a_partial_nigrostriatal_degeneration_induced_by_the_striatal_injection_of_different_doses_of_6_hydroxydopamine_ DB - PRIME DP - Unbound Medicine ER -