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Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue.
Biochem Biophys Res Commun. 2001 Nov 16; 288(5):1102-7.BB

Abstract

Adiponectin (ApN) is thought to play a major role in the pathogenesis of the Metabolic Syndrome. Production of ApN and regulation of its related gene (apM1) have not yet been studied in human visceral adipose tissue. ApN was mainly associated with adipocyte membranes and abundantly secreted in medium from isolated adipocytes. apM1 gene expression, restricted to the adipocyte fraction of adipose tissue, decreased spontaneously when adipose explants were cultured in basal medium for 24 h while the expression of other adipose genes barely changed (PPARgamma, GAPDH) or increased (PAI-1). Unexpectedly, the fall of apM1 mRNA was prevented by the addition of actinomycin D, an inhibitor of transcription, or cycloheximide, an inhibitor of protein synthesis, and by reducing the amount of adipose tissue cultured per dish, thereby suggesting that a newly synthesized factor released by adipose tissue destabilizes apM1 mRNA. apM1 gene expression was also negatively regulated by glucocorticoids and positively by insulin and IGF-1. This regulation could contribute to the decreased apM1/ApN levels in insulin-resistant patients with obesity and the Metabolic Syndrome.

Authors+Show Affiliations

Endocrinology and Metabolism Unit, University of Louvain, UCL 5530 Avenue Hippocrate, 55, B-1200 Brussels, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11700024

Citation

Halleux, C M., et al. "Secretion of Adiponectin and Regulation of apM1 Gene Expression in Human Visceral Adipose Tissue." Biochemical and Biophysical Research Communications, vol. 288, no. 5, 2001, pp. 1102-7.
Halleux CM, Takahashi M, Delporte ML, et al. Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue. Biochem Biophys Res Commun. 2001;288(5):1102-7.
Halleux, C. M., Takahashi, M., Delporte, M. L., Detry, R., Funahashi, T., Matsuzawa, Y., & Brichard, S. M. (2001). Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue. Biochemical and Biophysical Research Communications, 288(5), 1102-7.
Halleux CM, et al. Secretion of Adiponectin and Regulation of apM1 Gene Expression in Human Visceral Adipose Tissue. Biochem Biophys Res Commun. 2001 Nov 16;288(5):1102-7. PubMed PMID: 11700024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue. AU - Halleux,C M, AU - Takahashi,M, AU - Delporte,M L, AU - Detry,R, AU - Funahashi,T, AU - Matsuzawa,Y, AU - Brichard,S M, PY - 2001/11/9/pubmed PY - 2002/1/5/medline PY - 2001/11/9/entrez SP - 1102 EP - 7 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 288 IS - 5 N2 - Adiponectin (ApN) is thought to play a major role in the pathogenesis of the Metabolic Syndrome. Production of ApN and regulation of its related gene (apM1) have not yet been studied in human visceral adipose tissue. ApN was mainly associated with adipocyte membranes and abundantly secreted in medium from isolated adipocytes. apM1 gene expression, restricted to the adipocyte fraction of adipose tissue, decreased spontaneously when adipose explants were cultured in basal medium for 24 h while the expression of other adipose genes barely changed (PPARgamma, GAPDH) or increased (PAI-1). Unexpectedly, the fall of apM1 mRNA was prevented by the addition of actinomycin D, an inhibitor of transcription, or cycloheximide, an inhibitor of protein synthesis, and by reducing the amount of adipose tissue cultured per dish, thereby suggesting that a newly synthesized factor released by adipose tissue destabilizes apM1 mRNA. apM1 gene expression was also negatively regulated by glucocorticoids and positively by insulin and IGF-1. This regulation could contribute to the decreased apM1/ApN levels in insulin-resistant patients with obesity and the Metabolic Syndrome. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/11700024/Secretion_of_adiponectin_and_regulation_of_apM1_gene_expression_in_human_visceral_adipose_tissue_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(01)95904-2 DB - PRIME DP - Unbound Medicine ER -