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Sialyl Lewis(x) hybridized complement receptor type 1 moderates acid aspiration injury.
Am J Physiol Lung Cell Mol Physiol. 2001 Dec; 281(6):L1494-9.AJ

Abstract

The potentially enhanced anti-inflammatory effects of the sialyl Lewis(x) (sLe(x))-decorated version of soluble complement receptor type 1 (sCR1) in moderating acid aspiration injury are examined. HCl was instilled in tracheostomy tubes placed in mice, and extravasation of (125)I-labeled albumin in bronchoalveolar lavage (BAL) fluid was used to calculate the vascular permeability index (PI). Neutrophil counts in BAL fluid and immunohistochemistry were performed. PI was moderated by 82% after treatment with sCR1sLe(x) compared with 54% in sCR1-untreated mice (P < 0.05). Respective reductions in PI in mice treated 0.5 and 1 h after acid aspiration with sCR1sLe(x) of 70 and 57% were greater than the decreases in PI of 45 and 38% observed in respective sCR1-treated groups (P < 0.05). BAL fluid neutrophil counts in sCR1sLe(x)-treated mice were significantly less than those in sCR1-treated animals, which were similar to those in untreated mice. Immunohistochemistry stained for sCR1 only on the pulmonary vascular endothelium of sCR1sLe(x)- but not sCR1-treated mice. In conclusion, sCR1sLe(x) moderates permeability by antagonizing complement activation and neutrophil adhesion. Delayed complement and neutrophil antagonism significantly reduces injury.

Authors+Show Affiliations

Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11704546

Citation

Kyriakides, C, et al. "Sialyl Lewis(x) Hybridized Complement Receptor Type 1 Moderates Acid Aspiration Injury." American Journal of Physiology. Lung Cellular and Molecular Physiology, vol. 281, no. 6, 2001, pp. L1494-9.
Kyriakides C, Wang Y, Austen WG, et al. Sialyl Lewis(x) hybridized complement receptor type 1 moderates acid aspiration injury. Am J Physiol Lung Cell Mol Physiol. 2001;281(6):L1494-9.
Kyriakides, C., Wang, Y., Austen, W. G., Favuzza, J., Kobzik, L., Moore, F. D., & Hechtman, H. B. (2001). Sialyl Lewis(x) hybridized complement receptor type 1 moderates acid aspiration injury. American Journal of Physiology. Lung Cellular and Molecular Physiology, 281(6), L1494-9.
Kyriakides C, et al. Sialyl Lewis(x) Hybridized Complement Receptor Type 1 Moderates Acid Aspiration Injury. Am J Physiol Lung Cell Mol Physiol. 2001;281(6):L1494-9. PubMed PMID: 11704546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sialyl Lewis(x) hybridized complement receptor type 1 moderates acid aspiration injury. AU - Kyriakides,C, AU - Wang,Y, AU - Austen,W G,Jr AU - Favuzza,J, AU - Kobzik,L, AU - Moore,F D,Jr AU - Hechtman,H B, PY - 2001/11/13/pubmed PY - 2002/1/18/medline PY - 2001/11/13/entrez SP - L1494 EP - 9 JF - American journal of physiology. Lung cellular and molecular physiology JO - Am J Physiol Lung Cell Mol Physiol VL - 281 IS - 6 N2 - The potentially enhanced anti-inflammatory effects of the sialyl Lewis(x) (sLe(x))-decorated version of soluble complement receptor type 1 (sCR1) in moderating acid aspiration injury are examined. HCl was instilled in tracheostomy tubes placed in mice, and extravasation of (125)I-labeled albumin in bronchoalveolar lavage (BAL) fluid was used to calculate the vascular permeability index (PI). Neutrophil counts in BAL fluid and immunohistochemistry were performed. PI was moderated by 82% after treatment with sCR1sLe(x) compared with 54% in sCR1-untreated mice (P < 0.05). Respective reductions in PI in mice treated 0.5 and 1 h after acid aspiration with sCR1sLe(x) of 70 and 57% were greater than the decreases in PI of 45 and 38% observed in respective sCR1-treated groups (P < 0.05). BAL fluid neutrophil counts in sCR1sLe(x)-treated mice were significantly less than those in sCR1-treated animals, which were similar to those in untreated mice. Immunohistochemistry stained for sCR1 only on the pulmonary vascular endothelium of sCR1sLe(x)- but not sCR1-treated mice. In conclusion, sCR1sLe(x) moderates permeability by antagonizing complement activation and neutrophil adhesion. Delayed complement and neutrophil antagonism significantly reduces injury. SN - 1040-0605 UR - https://www.unboundmedicine.com/medline/citation/11704546/Sialyl_Lewis_x__hybridized_complement_receptor_type_1_moderates_acid_aspiration_injury_ L2 - https://journals.physiology.org/doi/10.1152/ajplung.2001.281.6.L1494?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -