Tags

Type your tag names separated by a space and hit enter

Effects of a four-day hyperinsulinemic-euglycemic clamp in early and mid-lactation dairy cows on plasma concentrations of metabolites, hormones, and binding proteins.
Domest Anim Endocrinol 2001; 21(3):169-85DA

Abstract

The effects of insulin, using a 4 d hyperinsulinemic-euglycemic clamp, on plasma concentrations of hormone, metabolites, and binding proteins were evaluated in four Holstein dairy cows during wk 4 and 17 of lactation. Insulin was infused at 1 microg/kg/hr for 96 hr during the clamp period. Compared with the pre-clamp period, plasma insulin concentrations increased 7-fold and 4-fold during the clamp periods in early and mid-lactation, respectively. The total amount of glucose infused was higher (P < 0.05) during the clamp in early lactation. The clamp decreased plasma concentrations of non-esterified fatty acids (P < 0.001) during early lactation while differences in mid-lactation were minor. The clamp also decreased plasma concentration of beta-hydroxybutyrate (P < 0.001), plasma urea nitrogen (P < 0.001), and true protein (P < 0.01) although the patterns of decline differed between early and mid-lactation. Growth hormone (GH) concentrations decreased (P < 0.001) and insulin-like growth factor-1 (IGF-1) increased (P < 0.01) during the clamp period suggesting a direct effect of insulin on the un-coupling of the GH/IGF-1 axis. Levels of IGF binding protein-2 (IGFBP-2) decreased (P < 0.01) during the clamp period. The relative proportion of IGFBP-2 decreased (P < 0.001) and that of IGFBP-3 increased (P < 0.001) during the clamp period. There were no interactions between the clamp period and stage of lactation on GH, IGF-1, or IGFBPs. Overall, most plasma variables measured were affected in the same way during the two clamps, but the pattern of change often varied with stage of lactation.

Authors+Show Affiliations

Danish Institute of Agricultural Sciences, Department of Animal Health and Welfare, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11707363

Citation

Mashek, D G., et al. "Effects of a Four-day Hyperinsulinemic-euglycemic Clamp in Early and Mid-lactation Dairy Cows On Plasma Concentrations of Metabolites, Hormones, and Binding Proteins." Domestic Animal Endocrinology, vol. 21, no. 3, 2001, pp. 169-85.
Mashek DG, Ingvartsen KL, Andersen JB, et al. Effects of a four-day hyperinsulinemic-euglycemic clamp in early and mid-lactation dairy cows on plasma concentrations of metabolites, hormones, and binding proteins. Domest Anim Endocrinol. 2001;21(3):169-85.
Mashek, D. G., Ingvartsen, K. L., Andersen, J. B., Vestergaard, M., & Larsen, T. (2001). Effects of a four-day hyperinsulinemic-euglycemic clamp in early and mid-lactation dairy cows on plasma concentrations of metabolites, hormones, and binding proteins. Domestic Animal Endocrinology, 21(3), pp. 169-85.
Mashek DG, et al. Effects of a Four-day Hyperinsulinemic-euglycemic Clamp in Early and Mid-lactation Dairy Cows On Plasma Concentrations of Metabolites, Hormones, and Binding Proteins. Domest Anim Endocrinol. 2001;21(3):169-85. PubMed PMID: 11707363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a four-day hyperinsulinemic-euglycemic clamp in early and mid-lactation dairy cows on plasma concentrations of metabolites, hormones, and binding proteins. AU - Mashek,D G, AU - Ingvartsen,K L, AU - Andersen,J B, AU - Vestergaard,M, AU - Larsen,T, PY - 2001/11/15/pubmed PY - 2002/1/15/medline PY - 2001/11/15/entrez SP - 169 EP - 85 JF - Domestic animal endocrinology JO - Domest. Anim. Endocrinol. VL - 21 IS - 3 N2 - The effects of insulin, using a 4 d hyperinsulinemic-euglycemic clamp, on plasma concentrations of hormone, metabolites, and binding proteins were evaluated in four Holstein dairy cows during wk 4 and 17 of lactation. Insulin was infused at 1 microg/kg/hr for 96 hr during the clamp period. Compared with the pre-clamp period, plasma insulin concentrations increased 7-fold and 4-fold during the clamp periods in early and mid-lactation, respectively. The total amount of glucose infused was higher (P < 0.05) during the clamp in early lactation. The clamp decreased plasma concentrations of non-esterified fatty acids (P < 0.001) during early lactation while differences in mid-lactation were minor. The clamp also decreased plasma concentration of beta-hydroxybutyrate (P < 0.001), plasma urea nitrogen (P < 0.001), and true protein (P < 0.01) although the patterns of decline differed between early and mid-lactation. Growth hormone (GH) concentrations decreased (P < 0.001) and insulin-like growth factor-1 (IGF-1) increased (P < 0.01) during the clamp period suggesting a direct effect of insulin on the un-coupling of the GH/IGF-1 axis. Levels of IGF binding protein-2 (IGFBP-2) decreased (P < 0.01) during the clamp period. The relative proportion of IGFBP-2 decreased (P < 0.001) and that of IGFBP-3 increased (P < 0.001) during the clamp period. There were no interactions between the clamp period and stage of lactation on GH, IGF-1, or IGFBPs. Overall, most plasma variables measured were affected in the same way during the two clamps, but the pattern of change often varied with stage of lactation. SN - 0739-7240 UR - https://www.unboundmedicine.com/medline/citation/11707363/Effects_of_a_four_day_hyperinsulinemic_euglycemic_clamp_in_early_and_mid_lactation_dairy_cows_on_plasma_concentrations_of_metabolites_hormones_and_binding_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0739-7240(01)00112-6 DB - PRIME DP - Unbound Medicine ER -