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Evidence for the interaction between nitric oxide and vasoactive intestinal polypeptide in the mouse gastric fundus.
J Pharmacol Exp Ther. 2001 Dec; 299(3):945-50.JP

Abstract

The involvement of nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) in nonadrenergic noncholinergic (NANC) nerve-induced relaxation and the interaction between NO and VIP were investigated in the mouse gastric fundus. N(omega)-nitro-L-arginine (L-NOARG; 100 microM) completely inhibited the NANC relaxations induced by electrical stimulation (ES) (0.5, 1, 2, 4, and 8 Hz; 25 V; 1 ms; 15-s trains). Hemoglobin (20 microM), hydroxocobalamin (100 microM), and 1H-[1,2,4,]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10 microM) diminished ES-induced relaxations, but alpha-chymotrypsin (10 U/ml) and VIP antiserum (1/200 dilution) had no effect on NANC relaxations. L-NOARG (100 microM) did not have any effect, whereas ODQ (10 microM) attenuated sodium nitroprusside (SNP; 100 nM)-induced relaxations. alpha-Chymotrypsin (10 U/ml) had no effect on the response to SNP. Furthermore, alpha-chymotrypsin (10 U/ml) abolished and VIP antiserum (1/200 dilution) diminished VIP (50 nM)-induced relaxations. L-NOARG (100 microM) caused an inhibition of VIP-induced relaxation that was reversed by L-arginine (1 mM) but not by D-arginine (1 mM). Similarly, ODQ (10 microM) inhibited the responses to VIP. 2-Amino-5,6-dihydro-6-methyl-4H-1,3-thiazine (5 microM) had no effect on these relaxations. L-NOARG (100 microM) and ODQ (10 microM) did not affect isoproterenol (10 nM)-induced relaxations. In conclusion, these results provide evidence that NO is involved in NANC nerve-induced relaxation and the participation of VIP (and related neuropeptides) cannot be excluded in causing relaxation of mouse gastric fundus muscle strips. These findings support the idea that VIP directly stimulates the production of NO by increasing NOS activity and thereby activating soluble guanylyl cyclase in smooth muscle.

Authors+Show Affiliations

Cukurova University, Faculty of Medicine, Department of Pharmacology, Adana, Turkey. yusufergun@yahoo.comNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11714881

Citation

Ergün, Y, and N Oğülener. "Evidence for the Interaction Between Nitric Oxide and Vasoactive Intestinal Polypeptide in the Mouse Gastric Fundus." The Journal of Pharmacology and Experimental Therapeutics, vol. 299, no. 3, 2001, pp. 945-50.
Ergün Y, Oğülener N. Evidence for the interaction between nitric oxide and vasoactive intestinal polypeptide in the mouse gastric fundus. J Pharmacol Exp Ther. 2001;299(3):945-50.
Ergün, Y., & Oğülener, N. (2001). Evidence for the interaction between nitric oxide and vasoactive intestinal polypeptide in the mouse gastric fundus. The Journal of Pharmacology and Experimental Therapeutics, 299(3), 945-50.
Ergün Y, Oğülener N. Evidence for the Interaction Between Nitric Oxide and Vasoactive Intestinal Polypeptide in the Mouse Gastric Fundus. J Pharmacol Exp Ther. 2001;299(3):945-50. PubMed PMID: 11714881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for the interaction between nitric oxide and vasoactive intestinal polypeptide in the mouse gastric fundus. AU - Ergün,Y, AU - Oğülener,N, PY - 2001/11/21/pubmed PY - 2002/1/5/medline PY - 2001/11/21/entrez SP - 945 EP - 50 JF - The Journal of pharmacology and experimental therapeutics JO - J. Pharmacol. Exp. Ther. VL - 299 IS - 3 N2 - The involvement of nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) in nonadrenergic noncholinergic (NANC) nerve-induced relaxation and the interaction between NO and VIP were investigated in the mouse gastric fundus. N(omega)-nitro-L-arginine (L-NOARG; 100 microM) completely inhibited the NANC relaxations induced by electrical stimulation (ES) (0.5, 1, 2, 4, and 8 Hz; 25 V; 1 ms; 15-s trains). Hemoglobin (20 microM), hydroxocobalamin (100 microM), and 1H-[1,2,4,]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10 microM) diminished ES-induced relaxations, but alpha-chymotrypsin (10 U/ml) and VIP antiserum (1/200 dilution) had no effect on NANC relaxations. L-NOARG (100 microM) did not have any effect, whereas ODQ (10 microM) attenuated sodium nitroprusside (SNP; 100 nM)-induced relaxations. alpha-Chymotrypsin (10 U/ml) had no effect on the response to SNP. Furthermore, alpha-chymotrypsin (10 U/ml) abolished and VIP antiserum (1/200 dilution) diminished VIP (50 nM)-induced relaxations. L-NOARG (100 microM) caused an inhibition of VIP-induced relaxation that was reversed by L-arginine (1 mM) but not by D-arginine (1 mM). Similarly, ODQ (10 microM) inhibited the responses to VIP. 2-Amino-5,6-dihydro-6-methyl-4H-1,3-thiazine (5 microM) had no effect on these relaxations. L-NOARG (100 microM) and ODQ (10 microM) did not affect isoproterenol (10 nM)-induced relaxations. In conclusion, these results provide evidence that NO is involved in NANC nerve-induced relaxation and the participation of VIP (and related neuropeptides) cannot be excluded in causing relaxation of mouse gastric fundus muscle strips. These findings support the idea that VIP directly stimulates the production of NO by increasing NOS activity and thereby activating soluble guanylyl cyclase in smooth muscle. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/11714881/Evidence_for_the_interaction_between_nitric_oxide_and_vasoactive_intestinal_polypeptide_in_the_mouse_gastric_fundus_ L2 - http://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=11714881 DB - PRIME DP - Unbound Medicine ER -