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TNF-alpha, IL-6 and IFN-gamma secreted by bronchoalveolar leukocytes isolated from patients with bronchial asthma, complicated by fungal airways infections.
Arch Immunol Ther Exp (Warsz). 1998; 46(6):381-6.AI

Abstract

It is widely known that fungal airways infections may deteriorate the course of bronchial asthma. The mechanism of the phenomenon is still unclear. The aim of our study was to assess the effect of fungal infections on the secretion of selected cytokines by bronchoalveolar leukocytes. Five patients (group FA) with bronchial asthma and Candida albicans or Aspergillus fumigatus airways infections (confirmed by bronchoscopy and culture) were included in the study. All of them were on the chronic treatment with corticosteroids (10-20 mg of prednisone per day) and underwent several courses of therapy with antibiotics. The control groups comprised 5 previously untreated asthmatics without bronchial colonization with fungi (group A) as well as 5 healthy volunteers (group H). Leukocytes were isolated from bronchoalveolar lavage fluid (BALF) and cultured in the presence or absence of cytokine inducers such as phytohemagglutin L (PHA), lipo-polysaccharide (LPS) from E. coli. The activity of TNF-alpha, IL-6 and IFN-gamma were measured in the BAL cell culture supernatants by using specific bioassays. In comparison with healthy controls the spontaneous or induced secretion of cytokines were significantly augmented in patients from group A. In contrast the asthmatics who represented group FA demonstrated normal levels of spontaneous cytokine secretion. However, the tendency to increase LPS and PHA-induced production was observed in BAL leukocytes from the patients. The above results support the view that beneficial effect of corticosteroid treatment in bronchial asthma may act, at least in part, by inhibition of the high spontaneous secretion of proinflammatory cytokines. Nevertheless, fungal airways infections may lead to increase of LPS- or PHA-induced production of TNF-alpha, IL-6 or IFN-gamma (despite prednisone therapy) by prestimulation of the BAL cells with fungi.

Authors+Show Affiliations

Institute of Immunology and Experimental Therapy, Polish Academy of Science, Weigla, Wrocław, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

11715987

Citation

Cembrzyńska-Nowak, M, et al. "TNF-alpha, IL-6 and IFN-gamma Secreted By Bronchoalveolar Leukocytes Isolated From Patients With Bronchial Asthma, Complicated By Fungal Airways Infections." Archivum Immunologiae Et Therapiae Experimentalis, vol. 46, no. 6, 1998, pp. 381-6.
Cembrzyńska-Nowak M, Liebhart J, Banaszek B, et al. TNF-alpha, IL-6 and IFN-gamma secreted by bronchoalveolar leukocytes isolated from patients with bronchial asthma, complicated by fungal airways infections. Arch Immunol Ther Exp (Warsz). 1998;46(6):381-6.
Cembrzyńska-Nowak, M., Liebhart, J., Banaszek, B., Dobek, R., Bieńkowska, M., & Szklarz, E. (1998). TNF-alpha, IL-6 and IFN-gamma secreted by bronchoalveolar leukocytes isolated from patients with bronchial asthma, complicated by fungal airways infections. Archivum Immunologiae Et Therapiae Experimentalis, 46(6), 381-6.
Cembrzyńska-Nowak M, et al. TNF-alpha, IL-6 and IFN-gamma Secreted By Bronchoalveolar Leukocytes Isolated From Patients With Bronchial Asthma, Complicated By Fungal Airways Infections. Arch Immunol Ther Exp (Warsz). 1998;46(6):381-6. PubMed PMID: 11715987.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TNF-alpha, IL-6 and IFN-gamma secreted by bronchoalveolar leukocytes isolated from patients with bronchial asthma, complicated by fungal airways infections. AU - Cembrzyńska-Nowak,M, AU - Liebhart,J, AU - Banaszek,B, AU - Dobek,R, AU - Bieńkowska,M, AU - Szklarz,E, PY - 2001/11/22/pubmed PY - 2002/1/5/medline PY - 2001/11/22/entrez SP - 381 EP - 6 JF - Archivum immunologiae et therapiae experimentalis JO - Arch Immunol Ther Exp (Warsz) VL - 46 IS - 6 N2 - It is widely known that fungal airways infections may deteriorate the course of bronchial asthma. The mechanism of the phenomenon is still unclear. The aim of our study was to assess the effect of fungal infections on the secretion of selected cytokines by bronchoalveolar leukocytes. Five patients (group FA) with bronchial asthma and Candida albicans or Aspergillus fumigatus airways infections (confirmed by bronchoscopy and culture) were included in the study. All of them were on the chronic treatment with corticosteroids (10-20 mg of prednisone per day) and underwent several courses of therapy with antibiotics. The control groups comprised 5 previously untreated asthmatics without bronchial colonization with fungi (group A) as well as 5 healthy volunteers (group H). Leukocytes were isolated from bronchoalveolar lavage fluid (BALF) and cultured in the presence or absence of cytokine inducers such as phytohemagglutin L (PHA), lipo-polysaccharide (LPS) from E. coli. The activity of TNF-alpha, IL-6 and IFN-gamma were measured in the BAL cell culture supernatants by using specific bioassays. In comparison with healthy controls the spontaneous or induced secretion of cytokines were significantly augmented in patients from group A. In contrast the asthmatics who represented group FA demonstrated normal levels of spontaneous cytokine secretion. However, the tendency to increase LPS and PHA-induced production was observed in BAL leukocytes from the patients. The above results support the view that beneficial effect of corticosteroid treatment in bronchial asthma may act, at least in part, by inhibition of the high spontaneous secretion of proinflammatory cytokines. Nevertheless, fungal airways infections may lead to increase of LPS- or PHA-induced production of TNF-alpha, IL-6 or IFN-gamma (despite prednisone therapy) by prestimulation of the BAL cells with fungi. SN - 0004-069X UR - https://www.unboundmedicine.com/medline/citation/11715987/TNF_alpha_IL_6_and_IFN_gamma_secreted_by_bronchoalveolar_leukocytes_isolated_from_patients_with_bronchial_asthma_complicated_by_fungal_airways_infections_ L2 - https://www.diseaseinfosearch.org/result/633 DB - PRIME DP - Unbound Medicine ER -