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Detection of sporadic cases of hepatitis E virus (HEV) infection in China using immunoassays based on recombinant open reading frame 2 and 3 polypeptides from HEV genotype 4.
J Clin Microbiol. 2001 Dec; 39(12):4370-9.JC

Abstract

We reported previously on the complete sequence of hepatitis E virus (HEV) genotype 4, isolated from patients with sporadic cases of acute HEV infection in China. At least eight HEV genotypes have now been described worldwide, and further isolates await classification. Current immunoassays for the detection of anti-HEV antibodies are based on polypeptides from genotypes 1 and 2 only and may be inadequate for the reliable detection of other genotypes. Because genotypes 1 and 4 predominate in China, we wished to investigate the antigenic reactivities of HEV genotype 4 proteins. Four overlapping regions of open reading frame 2 (ORF2) (FB5, amino acids [aa] 1 to 130; E4, aa 67 to 308; F2-2, aa 288 to 461; E5, aa 414 to 672) and the entire ORF3 product were expressed in Escherichia coli as fusion proteins. Enzyme immunoassays based on each of the five purified polypeptides were evaluated with sera from patients with sporadic cases of acute HEV infection. Individual immunoassays derived from HEV genotype 4 detected more cases of acute hepatitis E than a commercial assay. Some serum samples, which were positive for anti-HEV immunoglobulin G only by assays based on HEV genotype 4, were positive for HEV RNA by reverse transcription-PCR. Polypeptide FB5, from the N terminus of ORF2, had the greatest immunoreactivity with sera from patients with acute hepatitis E. These data indicate that the N terminus of ORF2 may provide epitopes which are highly reactive with acute-phase sera and that assays based on genotypes 1 and 2 alone may be inadequate for the detection of HEV infection in China, where sporadic cases of HEV infection are caused predominantly by HEV genotypes 4 and 1.

Authors+Show Affiliations

Centre for Hepatology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11724847

Citation

Wang, Y, et al. "Detection of Sporadic Cases of Hepatitis E Virus (HEV) Infection in China Using Immunoassays Based On Recombinant Open Reading Frame 2 and 3 Polypeptides From HEV Genotype 4." Journal of Clinical Microbiology, vol. 39, no. 12, 2001, pp. 4370-9.
Wang Y, Zhang H, Li Z, et al. Detection of sporadic cases of hepatitis E virus (HEV) infection in China using immunoassays based on recombinant open reading frame 2 and 3 polypeptides from HEV genotype 4. J Clin Microbiol. 2001;39(12):4370-9.
Wang, Y., Zhang, H., Li, Z., Gu, W., Lan, H., Hao, W., Ling, R., Li, H., & Harrison, T. J. (2001). Detection of sporadic cases of hepatitis E virus (HEV) infection in China using immunoassays based on recombinant open reading frame 2 and 3 polypeptides from HEV genotype 4. Journal of Clinical Microbiology, 39(12), 4370-9.
Wang Y, et al. Detection of Sporadic Cases of Hepatitis E Virus (HEV) Infection in China Using Immunoassays Based On Recombinant Open Reading Frame 2 and 3 Polypeptides From HEV Genotype 4. J Clin Microbiol. 2001;39(12):4370-9. PubMed PMID: 11724847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of sporadic cases of hepatitis E virus (HEV) infection in China using immunoassays based on recombinant open reading frame 2 and 3 polypeptides from HEV genotype 4. AU - Wang,Y, AU - Zhang,H, AU - Li,Z, AU - Gu,W, AU - Lan,H, AU - Hao,W, AU - Ling,R, AU - Li,H, AU - Harrison,T J, PY - 2001/11/29/pubmed PY - 2002/2/15/medline PY - 2001/11/29/entrez SP - 4370 EP - 9 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 39 IS - 12 N2 - We reported previously on the complete sequence of hepatitis E virus (HEV) genotype 4, isolated from patients with sporadic cases of acute HEV infection in China. At least eight HEV genotypes have now been described worldwide, and further isolates await classification. Current immunoassays for the detection of anti-HEV antibodies are based on polypeptides from genotypes 1 and 2 only and may be inadequate for the reliable detection of other genotypes. Because genotypes 1 and 4 predominate in China, we wished to investigate the antigenic reactivities of HEV genotype 4 proteins. Four overlapping regions of open reading frame 2 (ORF2) (FB5, amino acids [aa] 1 to 130; E4, aa 67 to 308; F2-2, aa 288 to 461; E5, aa 414 to 672) and the entire ORF3 product were expressed in Escherichia coli as fusion proteins. Enzyme immunoassays based on each of the five purified polypeptides were evaluated with sera from patients with sporadic cases of acute HEV infection. Individual immunoassays derived from HEV genotype 4 detected more cases of acute hepatitis E than a commercial assay. Some serum samples, which were positive for anti-HEV immunoglobulin G only by assays based on HEV genotype 4, were positive for HEV RNA by reverse transcription-PCR. Polypeptide FB5, from the N terminus of ORF2, had the greatest immunoreactivity with sera from patients with acute hepatitis E. These data indicate that the N terminus of ORF2 may provide epitopes which are highly reactive with acute-phase sera and that assays based on genotypes 1 and 2 alone may be inadequate for the detection of HEV infection in China, where sporadic cases of HEV infection are caused predominantly by HEV genotypes 4 and 1. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11724847/Detection_of_sporadic_cases_of_hepatitis_E_virus__HEV__infection_in_China_using_immunoassays_based_on_recombinant_open_reading_frame_2_and_3_polypeptides_from_HEV_genotype_4_ DB - PRIME DP - Unbound Medicine ER -