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Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells.
J Lipid Res. 2001 Dec; 42(12):2069-76.JL

Abstract

In a randomized, cross-over feeding trial involving 10 men with polygenic hypercholesterolemia, a control, Mediterranean-type cholesterol-lowering diet, and a diet of similar composition in which walnuts replaced approximately 35% of energy from unsaturated fat, were given for 6 weeks each. Compared with the control diet, the walnut diet reduced serum total and LDL cholesterol by 4.2% (P = 0.176), and 6.0% (P = 0.087), respectively. No changes were observed in HDL cholesterol, triglycerides, and apolipoprotein A-I levels or in the relative proportion of protein, triglycerides, phospholipids, and cholesteryl esters in LDL particles. The apolipoprotein B level declined in parallel with LDL cholesterol (6.0% reduction). Whole LDL, particularly the triglyceride fraction, was enriched in polyunsaturated fatty acids from walnuts (linoleic and alpha-linolenic acids). In comparison with LDL obtained during the control diet, LDL obtained during the walnut diet showed a 50% increase in association rates to the LDL receptor in human hepatoma HepG2 cells. LDL uptake by HepG2 cells was correlated with alpha-linolenic acid content of the triglyceride plus cholesteryl ester fractions of LDL particles (r(2) = 0.42, P < 0.05). Changes in the quantity and quality of LDL lipid fatty acids after a walnut-enriched diet facilitate receptor-mediated LDL clearance and may contribute to the cholesterol-lowering effect of walnut consumption.

Authors+Show Affiliations

Pharmacology Unit, School of Pharmacy, University of Barcelona, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11734580

Citation

Muñoz, S, et al. "Walnut-enriched Diet Increases the Association of LDL From Hypercholesterolemic Men With Human HepG2 Cells." Journal of Lipid Research, vol. 42, no. 12, 2001, pp. 2069-76.
Muñoz S, Merlos M, Zambón D, et al. Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells. J Lipid Res. 2001;42(12):2069-76.
Muñoz, S., Merlos, M., Zambón, D., Rodríguez, C., Sabaté, J., Ros, E., & Laguna, J. C. (2001). Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells. Journal of Lipid Research, 42(12), 2069-76.
Muñoz S, et al. Walnut-enriched Diet Increases the Association of LDL From Hypercholesterolemic Men With Human HepG2 Cells. J Lipid Res. 2001;42(12):2069-76. PubMed PMID: 11734580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells. AU - Muñoz,S, AU - Merlos,M, AU - Zambón,D, AU - Rodríguez,C, AU - Sabaté,J, AU - Ros,E, AU - Laguna,J C, PY - 2001/12/6/pubmed PY - 2002/3/7/medline PY - 2001/12/6/entrez SP - 2069 EP - 76 JF - Journal of lipid research JO - J. Lipid Res. VL - 42 IS - 12 N2 - In a randomized, cross-over feeding trial involving 10 men with polygenic hypercholesterolemia, a control, Mediterranean-type cholesterol-lowering diet, and a diet of similar composition in which walnuts replaced approximately 35% of energy from unsaturated fat, were given for 6 weeks each. Compared with the control diet, the walnut diet reduced serum total and LDL cholesterol by 4.2% (P = 0.176), and 6.0% (P = 0.087), respectively. No changes were observed in HDL cholesterol, triglycerides, and apolipoprotein A-I levels or in the relative proportion of protein, triglycerides, phospholipids, and cholesteryl esters in LDL particles. The apolipoprotein B level declined in parallel with LDL cholesterol (6.0% reduction). Whole LDL, particularly the triglyceride fraction, was enriched in polyunsaturated fatty acids from walnuts (linoleic and alpha-linolenic acids). In comparison with LDL obtained during the control diet, LDL obtained during the walnut diet showed a 50% increase in association rates to the LDL receptor in human hepatoma HepG2 cells. LDL uptake by HepG2 cells was correlated with alpha-linolenic acid content of the triglyceride plus cholesteryl ester fractions of LDL particles (r(2) = 0.42, P < 0.05). Changes in the quantity and quality of LDL lipid fatty acids after a walnut-enriched diet facilitate receptor-mediated LDL clearance and may contribute to the cholesterol-lowering effect of walnut consumption. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/11734580/Walnut_enriched_diet_increases_the_association_of_LDL_from_hypercholesterolemic_men_with_human_HepG2_cells_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&amp;pmid=11734580 DB - PRIME DP - Unbound Medicine ER -