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Expression of carbamoylphosphate synthetase I and glutamine synthetase in hepatic organoids reconstructed by rat small hepatocytes and hepatic nonparenchymal cells.
Cell Tissue Res. 2001 Dec; 306(3):467-71.CT

Abstract

The objective of this study was to investigate the expression of carbamoylphosphate synthetase I (CPS) and glutamine synthetase (GS) in small hepatocyte colonies and whether the heterogeneous expression of the enzymes could be induced during the maturation of small hepatocytes. Small hepatocytes isolated from an adult rat liver were cultured and proliferated to form colonies. The expression of CPS and GS was examined using immunocytochemistry and immunoblotting. In this culture more than 99% of morphologically hepatic cells were positive for CPS and all small hepatocytes were negative for GS at day 5. CPS-positive cells dramatically decreased with time in culture, whereas GS-positive ones appeared and their number increased in the colonies. Two to 3 weeks after plating, colonies with rising and piled-up cells appeared and the number of such colonies reached about 25% of all colonies at day 30. In most rising and piled-up cells in colonies both proteins were strongly expressed, whereas many small hepatocytes in monolayer colonies did not express either protein. When small hepatocytes in monolayer colonies were overlayed with Matrigel, the cells gradually piled up and both CPS and GS proteins were dramatically induced. The expression of CPS and GS in small hepatocytes may interact with the extracellular matrix because the rising and piled-up cells appear to be induced by the extracellular matrix produced by hepatic nonparenchymal cells.

Authors+Show Affiliations

Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Chuo-ku, S-1, W-17, Sapporo 060-8556, Japan. tmitaka@cc.sapmed.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11735048

Citation

Mitaka, T, et al. "Expression of Carbamoylphosphate Synthetase I and Glutamine Synthetase in Hepatic Organoids Reconstructed By Rat Small Hepatocytes and Hepatic Nonparenchymal Cells." Cell and Tissue Research, vol. 306, no. 3, 2001, pp. 467-71.
Mitaka T, Sato F, Ikeda SI, et al. Expression of carbamoylphosphate synthetase I and glutamine synthetase in hepatic organoids reconstructed by rat small hepatocytes and hepatic nonparenchymal cells. Cell Tissue Res. 2001;306(3):467-71.
Mitaka, T., Sato, F., Ikeda, S. I., Sugimoto, S. I., Higaki, N., Hirata, K., Lamers, W. H., & Mochizuki, Y. (2001). Expression of carbamoylphosphate synthetase I and glutamine synthetase in hepatic organoids reconstructed by rat small hepatocytes and hepatic nonparenchymal cells. Cell and Tissue Research, 306(3), 467-71.
Mitaka T, et al. Expression of Carbamoylphosphate Synthetase I and Glutamine Synthetase in Hepatic Organoids Reconstructed By Rat Small Hepatocytes and Hepatic Nonparenchymal Cells. Cell Tissue Res. 2001;306(3):467-71. PubMed PMID: 11735048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of carbamoylphosphate synthetase I and glutamine synthetase in hepatic organoids reconstructed by rat small hepatocytes and hepatic nonparenchymal cells. AU - Mitaka,T, AU - Sato,F, AU - Ikeda,S I, AU - Sugimoto,S I, AU - Higaki,N, AU - Hirata,K, AU - Lamers,W H, AU - Mochizuki,Y, Y1 - 2001/10/09/ PY - 2000/11/20/received PY - 2001/08/21/accepted PY - 2001/12/6/pubmed PY - 2002/2/7/medline PY - 2001/12/6/entrez SP - 467 EP - 71 JF - Cell and tissue research JO - Cell Tissue Res VL - 306 IS - 3 N2 - The objective of this study was to investigate the expression of carbamoylphosphate synthetase I (CPS) and glutamine synthetase (GS) in small hepatocyte colonies and whether the heterogeneous expression of the enzymes could be induced during the maturation of small hepatocytes. Small hepatocytes isolated from an adult rat liver were cultured and proliferated to form colonies. The expression of CPS and GS was examined using immunocytochemistry and immunoblotting. In this culture more than 99% of morphologically hepatic cells were positive for CPS and all small hepatocytes were negative for GS at day 5. CPS-positive cells dramatically decreased with time in culture, whereas GS-positive ones appeared and their number increased in the colonies. Two to 3 weeks after plating, colonies with rising and piled-up cells appeared and the number of such colonies reached about 25% of all colonies at day 30. In most rising and piled-up cells in colonies both proteins were strongly expressed, whereas many small hepatocytes in monolayer colonies did not express either protein. When small hepatocytes in monolayer colonies were overlayed with Matrigel, the cells gradually piled up and both CPS and GS proteins were dramatically induced. The expression of CPS and GS in small hepatocytes may interact with the extracellular matrix because the rising and piled-up cells appear to be induced by the extracellular matrix produced by hepatic nonparenchymal cells. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/11735048/Expression_of_carbamoylphosphate_synthetase_I_and_glutamine_synthetase_in_hepatic_organoids_reconstructed_by_rat_small_hepatocytes_and_hepatic_nonparenchymal_cells_ L2 - https://dx.doi.org/10.1007/s004410100465 DB - PRIME DP - Unbound Medicine ER -