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Cicatricial pemphigoid: IgA and IgG autoantibodies target epitopes on both intra- and extracellular domains of bullous pemphigoid antigen 180.
Br J Dermatol. 2001 Nov; 145(5):778-83.BJ

Abstract

BACKGROUND

Cicatricial pemphigoid (CP) is an autoimmune subepidermal blistering disease where autoantibodies target various components of the dermal-epidermal junction, including the bullous pemphigoid antigen 180 (BP180).

OBJECTIVE

We determined the exact specificity of circulating IgG and IgA autoantibodies to BP180 in a large number of CP patients.

METHODS

Twenty-six consecutive CP sera were analysed by Western blotting using a panel of cell-derived and recombinant proteins covering the entire BP180 molecule.

RESULTS

Circulating autoantibodies were detected in all CP sera. Seven sera reacting with laminin-5 were excluded from further analyses; the remaining 19 sera recognized BP180, including six sera (32%) that showed only IgA reactivity to this protein. With the combined use of the soluble BP180 ectodomain (LAD-1) and recombinant BP180 NC16A, 16 of these 19 CP sera (84%) targeted BP180. IgG reactivity was preferentially found against NC16A, whereas IgA antibodies predominantly recognized LAD-1. Thirty-two per cent of the BP180-reative sera revealed reactivity with the intracellular domain of this protein.

CONCLUSIONS

Our findings demonstrate that autoantibodies in CP target epitopes on both extra- and intracellular domains of BP180 and highlight the importance of testing for both IgG and IgA reactivity in these patients' sera.

Authors+Show Affiliations

Department of Dermatology, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11736901

Citation

Schmidt, E, et al. "Cicatricial Pemphigoid: IgA and IgG Autoantibodies Target Epitopes On Both Intra- and Extracellular Domains of Bullous Pemphigoid Antigen 180." The British Journal of Dermatology, vol. 145, no. 5, 2001, pp. 778-83.
Schmidt E, Skrobek C, Kromminga A, et al. Cicatricial pemphigoid: IgA and IgG autoantibodies target epitopes on both intra- and extracellular domains of bullous pemphigoid antigen 180. Br J Dermatol. 2001;145(5):778-83.
Schmidt, E., Skrobek, C., Kromminga, A., Hashimoto, T., Messer, G., Bröcker, E. B., Yancey, K. B., & Zillikens, D. (2001). Cicatricial pemphigoid: IgA and IgG autoantibodies target epitopes on both intra- and extracellular domains of bullous pemphigoid antigen 180. The British Journal of Dermatology, 145(5), 778-83.
Schmidt E, et al. Cicatricial Pemphigoid: IgA and IgG Autoantibodies Target Epitopes On Both Intra- and Extracellular Domains of Bullous Pemphigoid Antigen 180. Br J Dermatol. 2001;145(5):778-83. PubMed PMID: 11736901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cicatricial pemphigoid: IgA and IgG autoantibodies target epitopes on both intra- and extracellular domains of bullous pemphigoid antigen 180. AU - Schmidt,E, AU - Skrobek,C, AU - Kromminga,A, AU - Hashimoto,T, AU - Messer,G, AU - Bröcker,E B, AU - Yancey,K B, AU - Zillikens,D, PY - 2001/12/12/pubmed PY - 2002/1/5/medline PY - 2001/12/12/entrez SP - 778 EP - 83 JF - The British journal of dermatology JO - Br J Dermatol VL - 145 IS - 5 N2 - BACKGROUND: Cicatricial pemphigoid (CP) is an autoimmune subepidermal blistering disease where autoantibodies target various components of the dermal-epidermal junction, including the bullous pemphigoid antigen 180 (BP180). OBJECTIVE: We determined the exact specificity of circulating IgG and IgA autoantibodies to BP180 in a large number of CP patients. METHODS: Twenty-six consecutive CP sera were analysed by Western blotting using a panel of cell-derived and recombinant proteins covering the entire BP180 molecule. RESULTS: Circulating autoantibodies were detected in all CP sera. Seven sera reacting with laminin-5 were excluded from further analyses; the remaining 19 sera recognized BP180, including six sera (32%) that showed only IgA reactivity to this protein. With the combined use of the soluble BP180 ectodomain (LAD-1) and recombinant BP180 NC16A, 16 of these 19 CP sera (84%) targeted BP180. IgG reactivity was preferentially found against NC16A, whereas IgA antibodies predominantly recognized LAD-1. Thirty-two per cent of the BP180-reative sera revealed reactivity with the intracellular domain of this protein. CONCLUSIONS: Our findings demonstrate that autoantibodies in CP target epitopes on both extra- and intracellular domains of BP180 and highlight the importance of testing for both IgG and IgA reactivity in these patients' sera. SN - 0007-0963 UR - https://www.unboundmedicine.com/medline/citation/11736901/Cicatricial_pemphigoid:_IgA_and_IgG_autoantibodies_target_epitopes_on_both_intra__and_extracellular_domains_of_bullous_pemphigoid_antigen_180_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-0963&date=2001&volume=145&issue=5&spage=778 DB - PRIME DP - Unbound Medicine ER -