Tags

Type your tag names separated by a space and hit enter

Neuronal ectopic expression of tyrosine hydroxylase in the mouse striatum by combined administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 3-nitropropionic acid.
Neuroscience. 2001; 108(4):601-10.N

Abstract

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a dopaminergic neurotoxin which inhibits mitochondrial complex I. 3-Nitropropionic acid (3-NPA) inhibits mitochondrial complex II and produces specific striatal lesions. In order to produce a combined striatal neuronal and dopaminergic afferent lesion, we administered both toxins simultaneously to the mouse. The combination brought about a lesion in the striatum that was not simply additive of the two combined toxins. Intriguingly, a group of striatal neurons became immunoreactive to tyrosine hydroxylase after day 1. Some of them were clearly visible up to the dendritic details. Immuno-electron microscopy indicated that the tyrosine hydroxylase-positive striatal neurons contained densely immunoreactive polyribosomes. Reverse transcriptase-polymerase chain reaction analysis indicated the up-regulation of tyrosine hydroxylase mRNA in the treated striatum. These neurons were also immunoreactive to aromatic L-amino acid decarboxylase.We conclude that the combined administration of MPTP and 3-NPA caused a more profound damage to the nigro-striatal dopaminergic system, and thus some striatal neurons capable of up-regulating tyrosine hydroxylase were induced to produce dopamine, probably to compensate for the dopamine depletion.

Authors+Show Affiliations

Department of Neurology, School of Medicine, Fukushima Medical University, Hikarigaoka 1, 960-1295, Fukushima, Japan. nack@fmu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11738497

Citation

Nakahara, T, et al. "Neuronal Ectopic Expression of Tyrosine Hydroxylase in the Mouse Striatum By Combined Administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 3-nitropropionic Acid." Neuroscience, vol. 108, no. 4, 2001, pp. 601-10.
Nakahara T, Yamamoto T, Endo K, et al. Neuronal ectopic expression of tyrosine hydroxylase in the mouse striatum by combined administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 3-nitropropionic acid. Neuroscience. 2001;108(4):601-10.
Nakahara, T., Yamamoto, T., Endo, K., & Kayama, H. (2001). Neuronal ectopic expression of tyrosine hydroxylase in the mouse striatum by combined administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 3-nitropropionic acid. Neuroscience, 108(4), 601-10.
Nakahara T, et al. Neuronal Ectopic Expression of Tyrosine Hydroxylase in the Mouse Striatum By Combined Administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 3-nitropropionic Acid. Neuroscience. 2001;108(4):601-10. PubMed PMID: 11738497.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal ectopic expression of tyrosine hydroxylase in the mouse striatum by combined administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 3-nitropropionic acid. AU - Nakahara,T, AU - Yamamoto,T, AU - Endo,K, AU - Kayama,H, PY - 2001/12/12/pubmed PY - 2002/3/15/medline PY - 2001/12/12/entrez SP - 601 EP - 10 JF - Neuroscience JO - Neuroscience VL - 108 IS - 4 N2 - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a dopaminergic neurotoxin which inhibits mitochondrial complex I. 3-Nitropropionic acid (3-NPA) inhibits mitochondrial complex II and produces specific striatal lesions. In order to produce a combined striatal neuronal and dopaminergic afferent lesion, we administered both toxins simultaneously to the mouse. The combination brought about a lesion in the striatum that was not simply additive of the two combined toxins. Intriguingly, a group of striatal neurons became immunoreactive to tyrosine hydroxylase after day 1. Some of them were clearly visible up to the dendritic details. Immuno-electron microscopy indicated that the tyrosine hydroxylase-positive striatal neurons contained densely immunoreactive polyribosomes. Reverse transcriptase-polymerase chain reaction analysis indicated the up-regulation of tyrosine hydroxylase mRNA in the treated striatum. These neurons were also immunoreactive to aromatic L-amino acid decarboxylase.We conclude that the combined administration of MPTP and 3-NPA caused a more profound damage to the nigro-striatal dopaminergic system, and thus some striatal neurons capable of up-regulating tyrosine hydroxylase were induced to produce dopamine, probably to compensate for the dopamine depletion. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/11738497/Neuronal_ectopic_expression_of_tyrosine_hydroxylase_in_the_mouse_striatum_by_combined_administration_of_1_methyl_4_phenyl_1236_tetrahydropyridine_and_3_nitropropionic_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306452201004419 DB - PRIME DP - Unbound Medicine ER -