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Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222.
Nat Struct Biol. 2002 Jan; 9(1):37-41.NS

Abstract

Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.

Authors+Show Affiliations

Laboratory of Molecular Biophysics, University of Oxford, The Rex Richards Building, South Parks Road, Oxford OX1 3QU, UK. jasper@biop.ox.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11740505

Citation

van Thor, Jasper J., et al. "Phototransformation of Green Fluorescent Protein With UV and Visible Light Leads to Decarboxylation of Glutamate 222." Nature Structural Biology, vol. 9, no. 1, 2002, pp. 37-41.
van Thor JJ, Gensch T, Hellingwerf KJ, et al. Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222. Nat Struct Biol. 2002;9(1):37-41.
van Thor, J. J., Gensch, T., Hellingwerf, K. J., & Johnson, L. N. (2002). Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222. Nature Structural Biology, 9(1), 37-41.
van Thor JJ, et al. Phototransformation of Green Fluorescent Protein With UV and Visible Light Leads to Decarboxylation of Glutamate 222. Nat Struct Biol. 2002;9(1):37-41. PubMed PMID: 11740505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222. AU - van Thor,Jasper J, AU - Gensch,Thomas, AU - Hellingwerf,Klaas J, AU - Johnson,Louise N, PY - 2001/12/12/pubmed PY - 2002/1/30/medline PY - 2001/12/12/entrez SP - 37 EP - 41 JF - Nature structural biology JO - Nat Struct Biol VL - 9 IS - 1 N2 - Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates. SN - 1072-8368 UR - https://www.unboundmedicine.com/medline/citation/11740505/Phototransformation_of_green_fluorescent_protein_with_UV_and_visible_light_leads_to_decarboxylation_of_glutamate_222_ DB - PRIME DP - Unbound Medicine ER -