Tags

Type your tag names separated by a space and hit enter

A parathyroid-hormone-related-protein (PTH-rP)-specific cytotoxic T cell response induced by in vitro stimulation of tumour-infiltrating lymphocytes derived from prostate cancer metastases, with epitope peptide-loaded autologous dendritic cells and low-dose IL-2.
Br J Cancer. 2001 Nov 30; 85(11):1722-30.BJ

Abstract

Bone metastases are one of the most common events in patients with prostate carcinoma. PTH-rP, a protein produced by prostate carcinoma and other epithelial cancers, is a key agent for the development of bone metastases. A PTH-rP-derived peptide, designated PTR-4 was identified, which is capable to bind HLA-A2.1 molecules and to generate PTH-rP-specific cytotoxic T cell (CTL) lines from healthy HLA-A2.1(+) individual peripheral-blood-mononuclear-cells (PBMC). In this model, we investigated the in vitro possibility of generating an efficient PTH-rP specific CTL response by cyclical stimulations with IL-2 and PTR-4 peptide-pulsed autologous dendritic cells (DC), of HLA-A2.1(+) tumour infiltrating lymphocytes (TIL) derived from a patient with metastatic prostate carcinoma. A T cell line generated in this way (called TM-PTR-4) had a CD3(+), CD5(+), CD4(-), CD8(+), CD45(Ro+), CD56(-) immunophenotype and a HLA-A2.1 restricted cytotoxic activity to PTR-4-peptide pulsed CIR-A2 (HLA-A2.1(+)) target cells, PTH-rP(+)/HLA-A2.1(+) CIR-A2 transfected with PTH-rP gene, prostate carcinoma LNCaP cells, and autologous metastatic prostate cancer cells (M-CaP). These lymphocytes were not cytotoxic to HLA-A2.1(+) targets not producing PTH-rP, such as peptide-unpulsed CIR-A2 and colon carcinoma SW-1463, cell lines. Our results provide evidence that PTR-4 peptide-pulsed autologous DC may break the tolerance of human TIL against the autologous tumour by inducing a PTH-rP-specific CTL immune reaction. In conclusion PTR-4 peptide-pulsed autologous DC may be a promising approach for vaccine-therapy and antigen-specific CTL adoptive immunotherapy of hormone-resistant prostrate cancer.

Authors+Show Affiliations

Division of Medical Oncology, 'Giorgio Segre', University of Siena, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11742494

Citation

Correale, P, et al. "A Parathyroid-hormone-related-protein (PTH-rP)-specific Cytotoxic T Cell Response Induced By in Vitro Stimulation of Tumour-infiltrating Lymphocytes Derived From Prostate Cancer Metastases, With Epitope Peptide-loaded Autologous Dendritic Cells and Low-dose IL-2." British Journal of Cancer, vol. 85, no. 11, 2001, pp. 1722-30.
Correale P, Micheli L, Vecchio MT, et al. A parathyroid-hormone-related-protein (PTH-rP)-specific cytotoxic T cell response induced by in vitro stimulation of tumour-infiltrating lymphocytes derived from prostate cancer metastases, with epitope peptide-loaded autologous dendritic cells and low-dose IL-2. Br J Cancer. 2001;85(11):1722-30.
Correale, P., Micheli, L., Vecchio, M. T., Sabatino, M., Petrioli, R., Pozzessere, D., Marsili, S., Giorgi, G., Lozzi, L., Neri, P., & Francini, G. (2001). A parathyroid-hormone-related-protein (PTH-rP)-specific cytotoxic T cell response induced by in vitro stimulation of tumour-infiltrating lymphocytes derived from prostate cancer metastases, with epitope peptide-loaded autologous dendritic cells and low-dose IL-2. British Journal of Cancer, 85(11), 1722-30.
Correale P, et al. A Parathyroid-hormone-related-protein (PTH-rP)-specific Cytotoxic T Cell Response Induced By in Vitro Stimulation of Tumour-infiltrating Lymphocytes Derived From Prostate Cancer Metastases, With Epitope Peptide-loaded Autologous Dendritic Cells and Low-dose IL-2. Br J Cancer. 2001 Nov 30;85(11):1722-30. PubMed PMID: 11742494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A parathyroid-hormone-related-protein (PTH-rP)-specific cytotoxic T cell response induced by in vitro stimulation of tumour-infiltrating lymphocytes derived from prostate cancer metastases, with epitope peptide-loaded autologous dendritic cells and low-dose IL-2. AU - Correale,P, AU - Micheli,L, AU - Vecchio,M T, AU - Sabatino,M, AU - Petrioli,R, AU - Pozzessere,D, AU - Marsili,S, AU - Giorgi,G, AU - Lozzi,L, AU - Neri,P, AU - Francini,G, PY - 2001/12/18/pubmed PY - 2002/1/24/medline PY - 2001/12/18/entrez SP - 1722 EP - 30 JF - British journal of cancer JO - Br. J. Cancer VL - 85 IS - 11 N2 - Bone metastases are one of the most common events in patients with prostate carcinoma. PTH-rP, a protein produced by prostate carcinoma and other epithelial cancers, is a key agent for the development of bone metastases. A PTH-rP-derived peptide, designated PTR-4 was identified, which is capable to bind HLA-A2.1 molecules and to generate PTH-rP-specific cytotoxic T cell (CTL) lines from healthy HLA-A2.1(+) individual peripheral-blood-mononuclear-cells (PBMC). In this model, we investigated the in vitro possibility of generating an efficient PTH-rP specific CTL response by cyclical stimulations with IL-2 and PTR-4 peptide-pulsed autologous dendritic cells (DC), of HLA-A2.1(+) tumour infiltrating lymphocytes (TIL) derived from a patient with metastatic prostate carcinoma. A T cell line generated in this way (called TM-PTR-4) had a CD3(+), CD5(+), CD4(-), CD8(+), CD45(Ro+), CD56(-) immunophenotype and a HLA-A2.1 restricted cytotoxic activity to PTR-4-peptide pulsed CIR-A2 (HLA-A2.1(+)) target cells, PTH-rP(+)/HLA-A2.1(+) CIR-A2 transfected with PTH-rP gene, prostate carcinoma LNCaP cells, and autologous metastatic prostate cancer cells (M-CaP). These lymphocytes were not cytotoxic to HLA-A2.1(+) targets not producing PTH-rP, such as peptide-unpulsed CIR-A2 and colon carcinoma SW-1463, cell lines. Our results provide evidence that PTR-4 peptide-pulsed autologous DC may break the tolerance of human TIL against the autologous tumour by inducing a PTH-rP-specific CTL immune reaction. In conclusion PTR-4 peptide-pulsed autologous DC may be a promising approach for vaccine-therapy and antigen-specific CTL adoptive immunotherapy of hormone-resistant prostrate cancer. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/11742494/A_parathyroid_hormone_related_protein__PTH_rP__specific_cytotoxic_T_cell_response_induced_by_in_vitro_stimulation_of_tumour_infiltrating_lymphocytes_derived_from_prostate_cancer_metastases_with_epitope_peptide_loaded_autologous_dendritic_cells_and_low_dose_IL_2_ L2 - http://dx.doi.org/10.1054/bjoc.2001.2136 DB - PRIME DP - Unbound Medicine ER -