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Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.).
J Agric Food Chem. 2001 Dec; 49(12):5848-51.JA

Abstract

Anthocyanin pigments in seed coats of black soybean (Glycine max (L.) Merr.) were extracted with 1% HCl-CH(3)OH, and the crude anthocyanin extract was purified by Shepadex LH-20 and Lichroprep RP-18 open-column chromatography. Three major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-visible, FABMS, (1)H and (13)C NMR, and by TLC). The complete structures of these anthocyanins were elucidated as delphinidin-3-glucoside, cyanidin-3-glucoside, and petunidin-3-glucoside. Among them, petunidin-3-glucoside was identified as a new anthocyanin in black soybeans. On the basis of RP-HPLC with a UV-vis detector, the contents of delphinidin-3-glucoside, cyanidin-3-glucoside, petunidin-3-glucoside, and total anthocyanins in seed coats of 10 black soybeans were found in the ranges of 0-3.71, 0.94-15.98, 0-1.41, and 1.58-20.18 mg/g, respectively. The results obtained in this study imply that the seed coats of black soybean can be used as a good source for cyanidin-3-glucoside and delphinidin-3-glucoside.

Authors+Show Affiliations

Choung MG
National Yeongnam Agricultural Experiment Station, Rural Development Administration, 1085, Naidong, Milyang, 627-803, Gyeongnam, Republic of Korea. cmg700@rda.go.kr
Baek IY
No affiliation info available
Kang ST
No affiliation info available
Han WY
No affiliation info available
Shin DC
No affiliation info available
Moon HP
No affiliation info available
Kang KH
No affiliation info available

MeSH

AnthocyaninsChromatography, High Pressure LiquidMagnetic Resonance SpectroscopyModels, MolecularMolecular ConformationSeedsSoybeans

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11743773

Citation

Choung, M G., et al. "Isolation and Determination of Anthocyanins in Seed Coats of Black Soybean (Glycine Max (L.) Merr.)." Journal of Agricultural and Food Chemistry, vol. 49, no. 12, 2001, pp. 5848-51.
Choung MG, Baek IY, Kang ST, et al. Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.). J Agric Food Chem. 2001;49(12):5848-51.
Choung, M. G., Baek, I. Y., Kang, S. T., Han, W. Y., Shin, D. C., Moon, H. P., & Kang, K. H. (2001). Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.). Journal of Agricultural and Food Chemistry, 49(12), 5848-51.
Choung MG, et al. Isolation and Determination of Anthocyanins in Seed Coats of Black Soybean (Glycine Max (L.) Merr.). J Agric Food Chem. 2001;49(12):5848-51. PubMed PMID: 11743773.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.). AU - Choung,M G, AU - Baek,I Y, AU - Kang,S T, AU - Han,W Y, AU - Shin,D C, AU - Moon,H P, AU - Kang,K H, PY - 2001/12/18/pubmed PY - 2002/2/28/medline PY - 2001/12/18/entrez SP - 5848 EP - 51 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 49 IS - 12 N2 - Anthocyanin pigments in seed coats of black soybean (Glycine max (L.) Merr.) were extracted with 1% HCl-CH(3)OH, and the crude anthocyanin extract was purified by Shepadex LH-20 and Lichroprep RP-18 open-column chromatography. Three major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-visible, FABMS, (1)H and (13)C NMR, and by TLC). The complete structures of these anthocyanins were elucidated as delphinidin-3-glucoside, cyanidin-3-glucoside, and petunidin-3-glucoside. Among them, petunidin-3-glucoside was identified as a new anthocyanin in black soybeans. On the basis of RP-HPLC with a UV-vis detector, the contents of delphinidin-3-glucoside, cyanidin-3-glucoside, petunidin-3-glucoside, and total anthocyanins in seed coats of 10 black soybeans were found in the ranges of 0-3.71, 0.94-15.98, 0-1.41, and 1.58-20.18 mg/g, respectively. The results obtained in this study imply that the seed coats of black soybean can be used as a good source for cyanidin-3-glucoside and delphinidin-3-glucoside. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/11743773/Isolation_and_determination_of_anthocyanins_in_seed_coats_of_black_soybean__Glycine_max__L___Merr___ L2 - https://doi.org/10.1021/jf010550w DB - PRIME DP - Unbound Medicine ER -