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Drugs of abuse in a non-conventional sample; detection of methamphetamine and its main metabolite, amphetamine in abusers' clothes by HPLC with UV and fluorescence detection.
Biomed Chromatogr. 2001 Nov; 15(7):457-63.BC

Abstract

In this paper, we report the detection of methamphetamine and its major metabolite, amphetamine, in garments belong to known-abusers. These compounds were extracted from the textile using a mixture of chloroform:propan-2-ol (3:1, v/v), derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride and separated using a reversed-phase high-performance liquid chromatography. The derivatives were detected by measuring either fluorescence at 440 nm or absorbance at 330 nm. By using 1-methyl-3-phenyl propylamine as an internal standard, calibration curves of spiked textile samples were linear over a wide range with correlation coefficients of 0.997 or better. Detection limits at a signal-to-noise ratio of 3 were less than or equal to 37.3 and 0.4 pg on column for the high-performance liquid chromatography-ultraviolet and -fluorescence detection methods, respectively. Intra- and inter-day variations at high and low concentrations (n > or = 3) were < or =12.7%. The developed methods were successfully applied to the determination of methamphetamine and amphetamine in clothes samples belong to abusers.

Authors+Show Affiliations

Department of Analytical Research for Pharmacoinformatics, Graduate School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11746242

Citation

Al-Dirbashi, O Y., et al. "Drugs of Abuse in a Non-conventional Sample; Detection of Methamphetamine and Its Main Metabolite, Amphetamine in Abusers' Clothes By HPLC With UV and Fluorescence Detection." Biomedical Chromatography : BMC, vol. 15, no. 7, 2001, pp. 457-63.
Al-Dirbashi OY, Ikeda K, Takahashi M, et al. Drugs of abuse in a non-conventional sample; detection of methamphetamine and its main metabolite, amphetamine in abusers' clothes by HPLC with UV and fluorescence detection. Biomed Chromatogr. 2001;15(7):457-63.
Al-Dirbashi, O. Y., Ikeda, K., Takahashi, M., Kuroda, N., Ikeda, S., & Nakashima, K. (2001). Drugs of abuse in a non-conventional sample; detection of methamphetamine and its main metabolite, amphetamine in abusers' clothes by HPLC with UV and fluorescence detection. Biomedical Chromatography : BMC, 15(7), 457-63.
Al-Dirbashi OY, et al. Drugs of Abuse in a Non-conventional Sample; Detection of Methamphetamine and Its Main Metabolite, Amphetamine in Abusers' Clothes By HPLC With UV and Fluorescence Detection. Biomed Chromatogr. 2001;15(7):457-63. PubMed PMID: 11746242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Drugs of abuse in a non-conventional sample; detection of methamphetamine and its main metabolite, amphetamine in abusers' clothes by HPLC with UV and fluorescence detection. AU - Al-Dirbashi,O Y, AU - Ikeda,K, AU - Takahashi,M, AU - Kuroda,N, AU - Ikeda,S, AU - Nakashima,K, PY - 2001/12/18/pubmed PY - 2002/2/1/medline PY - 2001/12/18/entrez SP - 457 EP - 63 JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. VL - 15 IS - 7 N2 - In this paper, we report the detection of methamphetamine and its major metabolite, amphetamine, in garments belong to known-abusers. These compounds were extracted from the textile using a mixture of chloroform:propan-2-ol (3:1, v/v), derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride and separated using a reversed-phase high-performance liquid chromatography. The derivatives were detected by measuring either fluorescence at 440 nm or absorbance at 330 nm. By using 1-methyl-3-phenyl propylamine as an internal standard, calibration curves of spiked textile samples were linear over a wide range with correlation coefficients of 0.997 or better. Detection limits at a signal-to-noise ratio of 3 were less than or equal to 37.3 and 0.4 pg on column for the high-performance liquid chromatography-ultraviolet and -fluorescence detection methods, respectively. Intra- and inter-day variations at high and low concentrations (n > or = 3) were < or =12.7%. The developed methods were successfully applied to the determination of methamphetamine and amphetamine in clothes samples belong to abusers. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/11746242/Drugs_of_abuse_in_a_non_conventional_sample L2 - https://doi.org/10.1002/bmc.94 DB - PRIME DP - Unbound Medicine ER -