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The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation.
Yeast. 2001 Nov; 18(15):1371-81.Y

Abstract

A comparison of catabolic capacity was made between S. cerevisiae cells subjected to 24 h carbon or nitrogen starvation. The cells were shifted to starvation conditions at the onset of respiratory growth on ethanol in aerobic batch cultures, using glucose as the carbon and energy source. The results showed that the catabolic capacity was preserved to a much larger extent during carbon compared to nitrogen starvation. Nitrogen starvation experiments were made in the presence of ethanol (not glucose) to exclude the effect of glucose transport inactivation (Busturia and Lagunas, 1986). Hence, the difference in catabolic capacity could not be attributed to differences in glucose transport capacity during these conditions. In order to understand the reason for this difference in starvation response, measurement of protein composition, adenine nucleotides, inorganic phosphate, polyphosphate and storage carbohydrates were performed. No clear correlation between any of these variables and catabolic capacity after starvation could be obtained. However, there was a positive correlation between total catabolic activity and intracellular ATP concentration when glucose was added to starved cells. The possible mechanism for this correlation, as well as what determines the ATP level, is discussed.

Authors+Show Affiliations

Department of Cell and Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11746599

Citation

Nilsson, A, et al. "The Catabolic Capacity of Saccharomyces Cerevisiae Is Preserved to a Higher Extent During Carbon Compared to Nitrogen Starvation." Yeast (Chichester, England), vol. 18, no. 15, 2001, pp. 1371-81.
Nilsson A, Påhlman IL, Jovall PA, et al. The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation. Yeast. 2001;18(15):1371-81.
Nilsson, A., Påhlman, I. L., Jovall, P. A., Blomberg, A., Larsson, C., & Gustafsson, L. (2001). The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation. Yeast (Chichester, England), 18(15), 1371-81.
Nilsson A, et al. The Catabolic Capacity of Saccharomyces Cerevisiae Is Preserved to a Higher Extent During Carbon Compared to Nitrogen Starvation. Yeast. 2001;18(15):1371-81. PubMed PMID: 11746599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation. AU - Nilsson,A, AU - Påhlman,I L, AU - Jovall,P A, AU - Blomberg,A, AU - Larsson,C, AU - Gustafsson,L, PY - 2001/12/18/pubmed PY - 2002/3/8/medline PY - 2001/12/18/entrez SP - 1371 EP - 81 JF - Yeast (Chichester, England) JO - Yeast VL - 18 IS - 15 N2 - A comparison of catabolic capacity was made between S. cerevisiae cells subjected to 24 h carbon or nitrogen starvation. The cells were shifted to starvation conditions at the onset of respiratory growth on ethanol in aerobic batch cultures, using glucose as the carbon and energy source. The results showed that the catabolic capacity was preserved to a much larger extent during carbon compared to nitrogen starvation. Nitrogen starvation experiments were made in the presence of ethanol (not glucose) to exclude the effect of glucose transport inactivation (Busturia and Lagunas, 1986). Hence, the difference in catabolic capacity could not be attributed to differences in glucose transport capacity during these conditions. In order to understand the reason for this difference in starvation response, measurement of protein composition, adenine nucleotides, inorganic phosphate, polyphosphate and storage carbohydrates were performed. No clear correlation between any of these variables and catabolic capacity after starvation could be obtained. However, there was a positive correlation between total catabolic activity and intracellular ATP concentration when glucose was added to starved cells. The possible mechanism for this correlation, as well as what determines the ATP level, is discussed. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/11746599/The_catabolic_capacity_of_Saccharomyces_cerevisiae_is_preserved_to_a_higher_extent_during_carbon_compared_to_nitrogen_starvation_ L2 - https://doi.org/10.1002/yea.786 DB - PRIME DP - Unbound Medicine ER -