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Detection of specific Bacillus anthracis spore biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2001; 15(22):2110-6.RC

Abstract

Analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was applied for the characterization of Bacillus anthracis spore biomarkers. B. anthracis spores were extracted under a simple procedure, followed by linear mode analysis, using sinapinic acid as the matrix. Several markers with a mass range of 4-7 kDa were detected in three B. anthracis strains: Vollum, Sterne and V770-NP1-R. Similar spectra were also obtained for spore extracts of two members of the B. cereus group: B. thuringiensis and B. cereus, but not for B. mycoides, B. subtilis or B. licheniformis, suggesting that these markers are specific to closely related members of the B. cereus group. When alpha-cyano-4-hydroxycinnamic acid was used as the matrix, at least four additional new markers within a mass range of 2-4 kDa could be detected in all B. anthracis spore extracts. These markers, corresponding to a molecular weight of 2528.3, 2792.4, 3077.4, and 3590.7 Da, have not been observed in extracts of the three closely related Bacillus species - B. cereus, B. thuringiensis and B. mycoides. These unique B. anthracis biomarkers, which were isotopically resolved and reproducibly detected in the highly accurate MALDI-TOFMS reflectron mode, may be useful as a basis for rapid and specific identification of B. anthracis strains.

Authors+Show Affiliations

Elhanany E
Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel. elhanany@iibr.gov.il
Barak R
No affiliation info available
Fisher M
No affiliation info available
Kobiler D
No affiliation info available
Altboum Z
No affiliation info available

MeSH

Bacillus anthracisBacillus cereusBacillus thuringiensisBacterial ProteinsBiomarkersCoumaric AcidsReproducibility of ResultsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpores, Bacterial

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11746875

Citation

Elhanany, E, et al. "Detection of Specific Bacillus Anthracis Spore Biomarkers By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 15, no. 22, 2001, pp. 2110-6.
Elhanany E, Barak R, Fisher M, et al. Detection of specific Bacillus anthracis spore biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2001;15(22):2110-6.
Elhanany, E., Barak, R., Fisher, M., Kobiler, D., & Altboum, Z. (2001). Detection of specific Bacillus anthracis spore biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 15(22), 2110-6.
Elhanany E, et al. Detection of Specific Bacillus Anthracis Spore Biomarkers By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2001;15(22):2110-6. PubMed PMID: 11746875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of specific Bacillus anthracis spore biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. AU - Elhanany,E, AU - Barak,R, AU - Fisher,M, AU - Kobiler,D, AU - Altboum,Z, PY - 2001/12/18/pubmed PY - 2002/1/30/medline PY - 2001/12/18/entrez SP - 2110 EP - 6 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 15 IS - 22 N2 - Analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was applied for the characterization of Bacillus anthracis spore biomarkers. B. anthracis spores were extracted under a simple procedure, followed by linear mode analysis, using sinapinic acid as the matrix. Several markers with a mass range of 4-7 kDa were detected in three B. anthracis strains: Vollum, Sterne and V770-NP1-R. Similar spectra were also obtained for spore extracts of two members of the B. cereus group: B. thuringiensis and B. cereus, but not for B. mycoides, B. subtilis or B. licheniformis, suggesting that these markers are specific to closely related members of the B. cereus group. When alpha-cyano-4-hydroxycinnamic acid was used as the matrix, at least four additional new markers within a mass range of 2-4 kDa could be detected in all B. anthracis spore extracts. These markers, corresponding to a molecular weight of 2528.3, 2792.4, 3077.4, and 3590.7 Da, have not been observed in extracts of the three closely related Bacillus species - B. cereus, B. thuringiensis and B. mycoides. These unique B. anthracis biomarkers, which were isotopically resolved and reproducibly detected in the highly accurate MALDI-TOFMS reflectron mode, may be useful as a basis for rapid and specific identification of B. anthracis strains. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/11746875/Detection_of_specific_Bacillus_anthracis_spore_biomarkers_by_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.491 DB - PRIME DP - Unbound Medicine ER -
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