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Determination of midazolam in human plasma by solid-phase microextraction and gas chromatography/mass spectrometry.
Rapid Commun Mass Spectrom. 2001; 15(24):2497-501.RC

Abstract

A new method is described for the qualitative and quantitative analysis of midazolam, a short-acting 1,4-imidazole benzodiazepine, in human plasma. It involves a plasma deproteinization step, solid-phase microextraction (SPME) of midazolam using an 85-microm polyacrylate fiber, and its detection by gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring (SIM) mode, using pinazepam as internal standard. The assay is linear over a midazolam plasma range of 1.5-300 ng/mL, relative intra- and inter-assay standard deviations at 5 ng/mL are below 7%, and the limit of detection is 1 ng/mL. The method is simple, fast and sufficiently sensitive to be applied in clinical and forensic toxicology as well as for purposes of therapeutic drug monitoring.

Authors+Show Affiliations

Frison G
Forensic Toxicology and Antidoping, University Hospital of Padova, Via Falloppio 50, I-35121 Padova, Italy.
Tedeschi L
No affiliation info available
Maietti S
No affiliation info available
Ferrara SD
No affiliation info available

MeSH

Anti-Anxiety AgentsCalibrationGas Chromatography-Mass SpectrometryHumansHypnotics and SedativesMidazolam

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11746922

Citation

Frison, G, et al. "Determination of Midazolam in Human Plasma By Solid-phase Microextraction and Gas Chromatography/mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 15, no. 24, 2001, pp. 2497-501.
Frison G, Tedeschi L, Maietti S, et al. Determination of midazolam in human plasma by solid-phase microextraction and gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 2001;15(24):2497-501.
Frison, G., Tedeschi, L., Maietti, S., & Ferrara, S. D. (2001). Determination of midazolam in human plasma by solid-phase microextraction and gas chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 15(24), 2497-501.
Frison G, et al. Determination of Midazolam in Human Plasma By Solid-phase Microextraction and Gas Chromatography/mass Spectrometry. Rapid Commun Mass Spectrom. 2001;15(24):2497-501. PubMed PMID: 11746922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of midazolam in human plasma by solid-phase microextraction and gas chromatography/mass spectrometry. AU - Frison,G, AU - Tedeschi,L, AU - Maietti,S, AU - Ferrara,S D, PY - 2001/12/18/pubmed PY - 2002/3/19/medline PY - 2001/12/18/entrez SP - 2497 EP - 501 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 15 IS - 24 N2 - A new method is described for the qualitative and quantitative analysis of midazolam, a short-acting 1,4-imidazole benzodiazepine, in human plasma. It involves a plasma deproteinization step, solid-phase microextraction (SPME) of midazolam using an 85-microm polyacrylate fiber, and its detection by gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring (SIM) mode, using pinazepam as internal standard. The assay is linear over a midazolam plasma range of 1.5-300 ng/mL, relative intra- and inter-assay standard deviations at 5 ng/mL are below 7%, and the limit of detection is 1 ng/mL. The method is simple, fast and sufficiently sensitive to be applied in clinical and forensic toxicology as well as for purposes of therapeutic drug monitoring. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/11746922/Determination_of_midazolam_in_human_plasma_by_solid_phase_microextraction_and_gas_chromatography/mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.536 DB - PRIME DP - Unbound Medicine ER -